PMID- 28959649
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220216
IS  - 2214-7500 (Print)
IS  - 2214-7500 (Electronic)
IS  - 2214-7500 (Linking)
VI  - 4
DP  - 2017
TI  - In vitro biological assessment of Homalium zeylanicum and isolation of lucidenic 
      acid A triterpenoid.
PG  - 274-281
LID - 10.1016/j.toxrep.2017.04.004 [doi]
AB  - Homalium zeylanicum (Gardner) Benth. (Flacourtiaceae) is a medicinal plant useful 
      in controlling rheumatism, inflammation and diabetes. The objective of this work 
      evaluates in vitro antioxidant, antidiabetic, and antiinflammatory properties of 
      hydroalcohol extract of bark of H. zeylanicum (HAHZ). It also describes isolation 
      and structure determination of lucidenic acid A, which is the first report in 
      this plant. In order to explain the role of antioxidant principles, DPPH, nitric 
      oxide, hydroxyl, superoxide and metal chelating assays were performed. 
      Antidiabetic and anti-inflammatory activities were investigated by quantifying 
      alpha-amylase, alpha-glucosidase and protein denaturation inhibitory activities of HAHZ. 
      Biochemical estimations were performed. The chemical structure of the 
      triterpenoid was elucidated using (1)H, (13)C NMR and high resolution-MS. IC(50) 
      of DPPH, nitric oxide, hydroxyl, superoxide and metal chelating activities were 
      of 36.23 +/- 0.27, 40.11 +/- 0.32, 35.23 +/- 0.57, 43.34 +/- 0.22 and 11.54 +/- 0.08 mug/mL, 
      respectively. IC(50) of alpha-amylase and alpha-glucosidase activities were 29.12 +/- 0.54, 
      and 18.55 +/- 0.15 mug/mL. Total phenolic and total flavonoid contents were recorded 
      at 233.65 mg/g GAE and 172.7 mg/g QE. Regarding kinetic behaviour, HAHZ showed 
      competitive inhibition on alpha-glucosidase and mixed competitive inhibition on 
      alpha-amylase. Lucidenic acid A was confirmed by spectroscopic studies. 
      Anti-inflammatory activity of lucidenic acid A was determined by using protein 
      denaturation assay with IC(50) 13 mug/mL but HAHZ showed 30.34 +/- 0.13 mug/mL. 
      Phenols and flavonoids could be attributed to inhibition of intestinal 
      carbohydrases for anti-diabetic activities whereas triterpenoids could be 
      responsible for anti-inflammatory activity of H. zeylanicum.
FAU - Sahoo, Atish Kumar
AU  - Sahoo AK
AD  - Phytotherapy Research Lab., Medicinal and Aromatic Plant Division, Regional Plant 
      Resource Centre, Forest and Environment Department, Govt. of Odisha, Nayapalli, 
      Bhubaneswar, 751015, India.
FAU - Dash, Umesh Chandra
AU  - Dash UC
AD  - Phytotherapy Research Lab., Medicinal and Aromatic Plant Division, Regional Plant 
      Resource Centre, Forest and Environment Department, Govt. of Odisha, Nayapalli, 
      Bhubaneswar, 751015, India.
FAU - Kanhar, Satish
AU  - Kanhar S
AD  - Phytotherapy Research Lab., Medicinal and Aromatic Plant Division, Regional Plant 
      Resource Centre, Forest and Environment Department, Govt. of Odisha, Nayapalli, 
      Bhubaneswar, 751015, India.
FAU - Mahapatra, Ajay Kumar
AU  - Mahapatra AK
AD  - Odisha Forest Development Corp. Ltd., Forest and Environment Department, Govt. of 
      Odisha, A-84, Kharavela Nagar, Bhubaneswar, 751001, India.
LA  - eng
PT  - Journal Article
DEP - 20170604
PL  - Ireland
TA  - Toxicol Rep
JT  - Toxicology reports
JID - 101630272
PMC - PMC5615128
OTO - NOTNLM
OT  - &alpha
OT  - -amylase
OT  - -glucosidase
OT  - Antidiabetic
OT  - Antiinflammatory
OT  - DNS, dinitrosalicylic
OT  - DPPH, 2,2-diphenyl-1-picrylhydrazyl
OT  - Diclofenac sodium (PubChem CID: 5018304)
OT  - EDTA (PubChem CID: 6049)
OT  - GAeqv/g, gallic acid equivalents per gram
OT  - HAHZ, hydro alcoholic extract of Homalium zeylanicum
OT  - Homalium zeylanicum
OT  - IC50, half maximal inhibitory concentration
OT  - Lucidenic acid A (PubChem CID: 14109375)
OT  - Lucidenic acid a
OT  - NBT, nitroblue tetrazolium
OT  - NO, Nitric oxide
OT  - NSAIDs, nonsteroidal anti-inflammatory drugs
OT  - OH, hydroxyl
OT  - PBS, phosphate buffer saline
OT  - PMS, phenazine methosulphate
OT  - Queqv/g, Quercetin equivalents per gram
OT  - Quercetin (PubChem CID: 5280343)
OT  - ROS, reactive oxygen species
OT  - Rf, Retardation factor
OT  - SOD, superoxide anion
OT  - TFC, total flavonoid contents
OT  - TLC, thin layer chromatography
OT  - TPC, total phenolic contents
OT  - acarbose (PubChem CID: 41774)
OT  - gallic acid (PubChem CID: 370)
OT  - pNPG, p-nitrophenyl-alpha-d-glucopyranoside
COIS- The authors report no declarations of interest.
EDAT- 2017/09/30 06:00
MHDA- 2017/09/30 06:01
PMCR- 2017/06/04
CRDT- 2017/09/30 06:00
PHST- 2017/02/21 00:00 [received]
PHST- 2017/04/18 00:00 [revised]
PHST- 2017/04/23 00:00 [accepted]
PHST- 2017/09/30 06:00 [entrez]
PHST- 2017/09/30 06:00 [pubmed]
PHST- 2017/09/30 06:01 [medline]
PHST- 2017/06/04 00:00 [pmc-release]
AID - S2214-7500(17)30020-3 [pii]
AID - 10.1016/j.toxrep.2017.04.004 [doi]
PST - epublish
SO  - Toxicol Rep. 2017 Jun 4;4:274-281. doi: 10.1016/j.toxrep.2017.04.004. eCollection 
      2017.