PMID- 28963073
OWN - NLM
STAT- MEDLINE
DCOM- 20180206
LR  - 20181202
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 191
DP  - 2017 Nov
TI  - BM-MSCs-derived microvesicles promote allogeneic kidney graft survival through 
      enhancing micro-146a expression of dendritic cells.
PG  - 55-62
LID - S0165-2478(17)30060-3 [pii]
LID - 10.1016/j.imlet.2017.09.010 [doi]
AB  - OBJECTIVE: Microvesicles (MVs) are plasmalemmal vesicles that are released from 
      various cells and regarded as a mediator of intermolecular communication. In 
      present study, we aimed to evaluate the therapeutic efficacy of the bone marrow 
      mesenchymal stem cells (BM-MSCs)-derived MVs in the mice kidney transplant model 
      and explored the underlying mechanism. METHODS: BM-MSCs were isolated from 
      C57BL/6 mice and identified using flow cytometry. In vivo allogenic kidney 
      transplantation model of mice was performed between C57BL/6 mice (recipient) and 
      BALB/c mice (donor). Recipient-type BM-MSC (0.1ml) or equal volume of medium as a 
      control was injected i.v. 24h after kidney transplantation. Serum was collected 
      for creatinine concentration detection at 14 d after transplantation. Dendritic 
      cells (DCs) phenotype and miR-146a expression level in plant was identified. 
      Immature DCs (iDCs) and mature DCs (mDCs) were derived from monocytes. MVs were 
      separated from BM-MSCs. RESULTS: BM-MSCs positive for CD29 (95.8%) and CD44 
      (94.7%) were cultured and confirmed to prolong the allogenic kidney graft 
      survival in mice. Importantly, the expression of miR-146a increased significantly 
      in DCs of BM-MSCs-treated allogenic kidney. Moreover, both BM-MSCs and MVs 
      derived from BM-MSCs enhanced miR-146a expression in iDCs and mDCs in vitro. 
      Furthermore, MVs substantially reduced IL-12 mRNA expression and IL-12 production 
      of mDCs whereas this action was reversed by miR-146a silencing. MiR-146a 
      silencing also abrogated the MVs-induced decrease in serum creatinine, reduction 
      of immature DCs phenotype in transplant and increase in miR-146a expression 
      level. CONCLUSION: In summary, our data suggested that the BM-MSCs-derived MVs 
      improved allogenic kidney transplantation survival through inhibiting DCs 
      maturity by miR-146a.
CI  - Copyright (c) 2017 European Federation of Immunological Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Wu, Xiao-Qiang
AU  - Wu XQ
AD  - Department of Urology, Henan Provincial People's Hospital, Zhengzhou 450003, 
      China.
FAU - Yan, Tian-Zhong
AU  - Yan TZ
AD  - Department of Urology, Henan Provincial People's Hospital, Zhengzhou 450003, 
      China. Electronic address: yantzhong666@163.com.
FAU - Wang, Zhi-Wei
AU  - Wang ZW
AD  - Department of Urology, Henan Provincial People's Hospital, Zhengzhou 450003, 
      China.
FAU - Wu, Xuan
AU  - Wu X
AD  - Department of Urology, Henan Provincial People's Hospital, Zhengzhou 450003, 
      China.
FAU - Cao, Guang-Hui
AU  - Cao GH
AD  - Department of Urology, Henan Provincial People's Hospital, Zhengzhou 450003, 
      China.
FAU - Zhang, Chan
AU  - Zhang C
AD  - Department of Urology, Henan Provincial People's Hospital, Zhengzhou 450003, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20170928
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn146 microRNA, mouse)
RN  - 0 (RNA, Small Interfering)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*physiology
MH  - Cell Differentiation
MH  - Cell-Derived Microparticles/*metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/*physiology
MH  - Down-Regulation
MH  - Graft Survival/genetics
MH  - Interleukin-12/genetics/metabolism
MH  - *Kidney Transplantation
MH  - Mesenchymal Stem Cells/*metabolism/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/genetics/*metabolism
MH  - RNA, Small Interfering/genetics
MH  - Transplantation, Homologous
OTO - NOTNLM
OT  - Fas
OT  - IL-12
OT  - iDCs
OT  - mDCs
EDAT- 2017/10/01 06:00
MHDA- 2018/02/07 06:00
CRDT- 2017/10/01 06:00
PHST- 2017/02/07 00:00 [received]
PHST- 2017/09/05 00:00 [revised]
PHST- 2017/09/25 00:00 [accepted]
PHST- 2017/10/01 06:00 [pubmed]
PHST- 2018/02/07 06:00 [medline]
PHST- 2017/10/01 06:00 [entrez]
AID - S0165-2478(17)30060-3 [pii]
AID - 10.1016/j.imlet.2017.09.010 [doi]
PST - ppublish
SO  - Immunol Lett. 2017 Nov;191:55-62. doi: 10.1016/j.imlet.2017.09.010. Epub 2017 Sep 
      28.