PMID- 28963736
OWN - NLM
STAT- MEDLINE
DCOM- 20180824
LR  - 20181202
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 52
IP  - 6
DP  - 2017 Dec
TI  - Expression of GnRH receptor in the canine corpus luteum, and luteal function 
      following deslorelin acetate-induced puberty delay.
PG  - 1104-1112
LID - 10.1111/rda.13038 [doi]
AB  - The goals of this study were as follows: (Experiment 1) to examine the basic 
      capability of canine corpora lutea (CL) to respond to GnRH by assessing 
      expression of gonadotropin-releasing hormone receptor (GnRH-R) in luteal samples 
      collected throughout the luteal lifespan from non-pregnant dogs, and (Experiment 
      2) to investigate the effects of pre-pubertal application of the GnRH agonist 
      deslorelin acetate on luteal function following the first oestrus. Mature CL were 
      collected during the mid-luteal phase (days 30-45) from treated and control 
      bitches. Transcript levels of several factors were determined: estrogen receptors 
      (ESR1/ERalpha, ESR2/ERbeta), progesterone (P4)-receptor (PGR), prolactin receptor 
      (PRLR), PGE2-synthase (PTGES) and PGE2 receptors (PTGER2/EP2, PTGER4/EP4), 
      vascular endothelial growth factor (VEGFA) and VEGF receptors (VEGFR1 and 
      VEGFR2), cyclooxygenase 2 (COX2/PTGS2), steroidogenic acute regulatory protein 
      (STAR) and 3beta-hydroxysteroid dehydrogenase (3betaHSD). Additionally, levels of 
      Kisspeptin 1 (Kiss1) and its receptor (KISS1-R) were evaluated. Although 
      generally low, GnRH-R expression was time dependent and was elevated during early 
      dioestrus, with a significant decrease towards luteal regression. In 
      deslorelin-treated and control dogs, its expression was either low or frequently 
      below the detection limit. EP2 and VEGFR1 were higher in the treated group, which 
      could be caused by a feedback mechanism after long-term suppression of 
      reproductive activity. Despite large individual variations, 3betaHSD was higher in 
      the deslorelin-treated group. This, along with unchanged STAR expression, was 
      apparently not mirrored in increased luteal functionality, because similar P4 
      levels were detected in both groups. Finally, the deslorelin-mediated long-term 
      delay of puberty does not have negative carry-over effects on subsequent ovarian 
      functionality in bitches.
CI  - (c) 2017 Blackwell Verlag GmbH.
FAU - Kaya, D
AU  - Kaya D
AD  - Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, 
      University of Kafkas, Kars, Turkey.
FAU - Gram, A
AU  - Gram A
AUID- ORCID: 0000-0002-7490-5836
AD  - Vetsuisse Faculty, Institute of Veterinary Anatomy, University of Zurich, Zurich, 
      Switzerland.
FAU - Kowalewski, M P
AU  - Kowalewski MP
AD  - Vetsuisse Faculty, Institute of Veterinary Anatomy, University of Zurich, Zurich, 
      Switzerland.
FAU - Schafer-Somi, S
AU  - Schafer-Somi S
AD  - Platform for Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, 
      Vienna, Austria.
FAU - Kuru, M
AU  - Kuru M
AD  - Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, 
      University of Kafkas, Kars, Turkey.
FAU - Boos, A
AU  - Boos A
AD  - Vetsuisse Faculty, Institute of Veterinary Anatomy, University of Zurich, Zurich, 
      Switzerland.
FAU - Aslan, S
AU  - Aslan S
AD  - Department of Obstetrics and Gynecology, Veterinary Faculty, Near East 
      University, Nicosia, North Cyprus, Turkey.
LA  - eng
SI  - GENBANK/KJ512885
SI  - GENBANK/AB028142
SI  - GENBANK/AY739720.1
SI  - GENBANK/NM_001003074
SI  - GENBANK/EF522840
SI  - GENBANK/HQ110882
SI  - GENBANK/NM_001122854
SI  - GENBANK/NM001003175
SI  - GENBANK/AF262963
SI  - GENBANK/DQ269018
SI  - GENBANK/NM001048024
SI  - GENBANK/AF075602
SI  - GENBANK/AF177934
SI  - GENBANK/XM533454
SI  - GENBANK/XM861041
SI  - GENBANK/HQ267784
PT  - Journal Article
DEP - 20170930
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Kisspeptins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, LHRH)
RN  - 0 (Receptors, Steroid)
RN  - 08AN7WA2G0 (Triptorelin Pamoate)
RN  - TKG3I66TVE (deslorelin)
SB  - IM
MH  - Animals
MH  - Corpus Luteum/*drug effects/growth & development
MH  - Dogs
MH  - Female
MH  - Kisspeptins/analysis
MH  - Receptors, Cell Surface
MH  - Receptors, LHRH/*antagonists & inhibitors/*physiology
MH  - Receptors, Steroid
MH  - Sexual Maturation/drug effects
MH  - Triptorelin Pamoate/*analogs & derivatives/pharmacology
OTO - NOTNLM
OT  - GnRH receptor
OT  - deslorelin
OT  - domestic dog (Canis familiaris)
OT  - luteal function
OT  - puberty delay
EDAT- 2017/10/01 06:00
MHDA- 2018/08/25 06:00
CRDT- 2017/10/01 06:00
PHST- 2017/03/10 00:00 [received]
PHST- 2017/06/24 00:00 [accepted]
PHST- 2017/10/01 06:00 [pubmed]
PHST- 2018/08/25 06:00 [medline]
PHST- 2017/10/01 06:00 [entrez]
AID - 10.1111/rda.13038 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2017 Dec;52(6):1104-1112. doi: 10.1111/rda.13038. Epub 2017 
      Sep 30.