PMID- 28964407
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR  - 20181202
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 52
IP  - 12
DP  - 2017 Dec
TI  - Isolation of myogenic progenitor cell population from human placenta: A pilot 
      study.
PG  - 2078-2082
LID - S0022-3468(17)30522-5 [pii]
LID - 10.1016/j.jpedsurg.2017.08.025 [doi]
AB  - PURPOSE: The purpose of this study was to demonstrate a method of isolating 
      myogenic progenitor cells from human placenta chorionic villi and to confirm the 
      myogenic characteristics of the isolated cells. METHODS: Cells were isolated from 
      chorionic villi of a second trimester male placenta via a combined enzymatic 
      digestion and explant culture. A morphologically distinct subpopulation of 
      elongated and multinucleated cells was identified. This subpopulation was 
      manually passaged from the explant culture, expanded, and analyzed by 
      fluorescence in situ hybridization (FISH) assay, immunocytochemistry, and flow 
      cytometry. Myogenic characteristics including alignment and fusion were tested by 
      growing these cells on aligned polylactic acid microfibrous scaffold in a fusion 
      media composed of 2% horse serum in Dulbecco's modified Eagle medium/high 
      glucose. RESULTS: The expanded subpopulation was uniformly positive for integrin 
      alpha-7. Presence of Y-chromosome by FISH analysis confirmed chorionic villus origin 
      rather than maternal cell contamination. Isolated cells grew, aligned, and fused 
      on the microfibrous scaffold, and they expressed myogenin, desmin, and MHC 
      confirming their myogenic identity. CONCLUSION: Myogenic progenitor cells can be 
      isolated from human chorionic villi. This opens the possibility for translational 
      and clinical applications using autologous myogenic cells for possible 
      engraftment in treatment of chest and abdominal wall defects.
CI  - Copyright (c) 2017. Published by Elsevier Inc.
FAU - Kabagambe, Sandra K
AU  - Kabagambe SK
AD  - University of California, Davis Health, Sacramento, CA, USA. Electronic address: 
      skkabagambe@ucdavis.edu.
FAU - Lankford, Lee
AU  - Lankford L
AD  - University of California, Davis Health, Sacramento, CA, USA.
FAU - Kumar, Priyadarsini
AU  - Kumar P
AD  - University of California, Davis Health, Sacramento, CA, USA.
FAU - Chen, Y Julia
AU  - Chen YJ
AD  - University of California, Davis Health, Sacramento, CA, USA.
FAU - Herout, Kyle T
AU  - Herout KT
AD  - University of California, Davis Health, Sacramento, CA, USA.
FAU - Lee, Chelsey J
AU  - Lee CJ
AD  - University of California, Davis Health, Sacramento, CA, USA.
FAU - Stark, Rebecca A
AU  - Stark RA
AD  - University of California, Davis Health, Sacramento, CA, USA.
FAU - Farmer, Diana L
AU  - Farmer DL
AD  - University of California, Davis Health, Sacramento, CA, USA.
FAU - Wang, Aijun
AU  - Wang A
AD  - University of California, Davis Health, Sacramento, CA, USA.
LA  - eng
PT  - Journal Article
DEP - 20170904
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
SB  - IM
MH  - *Chorionic Villi
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Muscle Development
MH  - Pilot Projects
MH  - Placenta/*cytology
MH  - Pregnancy
MH  - Pregnancy Trimester, Second
MH  - *Stem Cells
OTO - NOTNLM
OT  - Chorionic villi
OT  - Diaphragmatic hernia
OT  - Myogenic progenitor cells
OT  - Placenta
OT  - Tissue engineering
EDAT- 2017/10/02 06:00
MHDA- 2018/07/10 06:00
CRDT- 2017/10/02 06:00
PHST- 2017/08/06 00:00 [received]
PHST- 2017/08/28 00:00 [accepted]
PHST- 2017/10/02 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
PHST- 2017/10/02 06:00 [entrez]
AID - S0022-3468(17)30522-5 [pii]
AID - 10.1016/j.jpedsurg.2017.08.025 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2017 Dec;52(12):2078-2082. doi: 10.1016/j.jpedsurg.2017.08.025. 
      Epub 2017 Sep 4.