PMID- 28964862
OWN - NLM
STAT- MEDLINE
DCOM- 20180625
LR  - 20180625
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 71
DP  - 2017 Dec
TI  - Omega-3 polyunsaturated fatty acids alleviate hepatic steatosis-induced 
      inflammation through Sirt1-mediated nuclear translocation of NF-kappaB p65 subunit in 
      hepatocytes of large yellow croaker (Larmichthys crocea).
PG  - 76-82
LID - S1050-4648(17)30584-3 [pii]
LID - 10.1016/j.fsi.2017.09.064 [doi]
AB  - Hepatic steatosis induced inflammation is becoming increasingly prevalent in 
      farmed fish. This study was conducted to investigate the protective effects of 
      omega-3 polyunsaturated fatty acids (omega-3 PUFAs) against hepatic steatosis-induced 
      inflammation and its potential molecular mechanisms in hepatocyte of large yellow 
      croaker (Larmichthys crocea). We found that the hepatic steatosis-induced 
      inflammation was relieved by omega-3 PUFAs, meanwhile, the Sirt1 activity and 
      transcript expression was increased by omega-3 PUFAs. The increased Sirt1 activity 
      can decrease the hepatic steatosis-induced inflammation. The protective effects 
      of omega-3 PUFAs against hepatic steatosis-induced inflammation was reversed by the 
      treatment with Sirt1 inhibitor EX-527. The nuclear translocation of nuclear 
      transcription factor kappa-B (NF-kappaB) p65 was significantly decreased after omega-3 
      PUFAs treatments compared to the palmitic acid stimulation group. The omega-3 PUFAs 
      induced cytoplasm translocation of NF-kappaB p65 was reversed by EX-527. Together, 
      omega-3 PUFAs alleviate hepatic steatosis-induced inflammation through Sirt1-mediated 
      nuclear translocation of NF-kappaB p65 subunit in hepatocytes of large yellow 
      croaker. The present study provides important insight into the mechanisms of the 
      protective effects of omega-3 PUFAs, providing theory bases for alleviating the 
      hepatic steatosis induced inflammation of farmed fish, thereby offering great 
      benefits to the aquaculture industry and fish consumers.
CI  - Copyright (c) 2017. Published by Elsevier Ltd.
FAU - Wang, Tianjiao
AU  - Wang T
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Yang, Bo
AU  - Yang B
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Ji, Renlei
AU  - Ji R
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Xu, Wei
AU  - Xu W
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Mai, Kangsen
AU  - Mai K
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
FAU - Ai, Qinghui
AU  - Ai Q
AD  - The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) 
      and Key Laboratory of Mariculture (Ministry of Education), Ocean University of 
      China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China. 
      Electronic address: qhai@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170928
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fish Proteins)
RN  - 0 (Transcription Factor RelA)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Fatty Acids, Omega-3/*pharmacology
MH  - Fatty Liver/etiology/*veterinary
MH  - Fish Diseases/genetics/*immunology
MH  - Fish Proteins/*genetics/metabolism
MH  - Hepatocytes
MH  - Inflammation/genetics/immunology/*veterinary
MH  - *Perciformes
MH  - Protein Transport
MH  - Sirtuin 1/genetics/metabolism
MH  - Transcription Factor RelA/*metabolism
OTO - NOTNLM
OT  - Hepatic steatosis
OT  - Inflammation
OT  - Large yellow croaker
OT  - NF-kappaB
OT  - Sirt1
EDAT- 2017/10/02 06:00
MHDA- 2018/06/26 06:00
CRDT- 2017/10/02 06:00
PHST- 2017/07/07 00:00 [received]
PHST- 2017/09/17 00:00 [revised]
PHST- 2017/09/26 00:00 [accepted]
PHST- 2017/10/02 06:00 [pubmed]
PHST- 2018/06/26 06:00 [medline]
PHST- 2017/10/02 06:00 [entrez]
AID - S1050-4648(17)30584-3 [pii]
AID - 10.1016/j.fsi.2017.09.064 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2017 Dec;71:76-82. doi: 10.1016/j.fsi.2017.09.064. Epub 
      2017 Sep 28.