PMID- 28966340
OWN - NLM
STAT- MEDLINE
DCOM- 20180705
LR  - 20191113
IS  - 2325-4416 (Electronic)
IS  - 2325-4394 (Print)
IS  - 2325-4394 (Linking)
VI  - 23
DP  - 2017 Oct 2
TI  - A New Clinically Relevant T-Score Standard to Interpret Bone Status in a Sheep 
      Model.
PG  - 326-335
AB  - BACKGROUND Osteoporosis is diagnosed by bone loss using a radiological parameter 
      called T-score. Preclinical studies use DXA to evaluate bone status were the 
      T-score is referenced on bone mineral density (BMD) values of the same animals 
      before treatment. Clinically, the reference BMD represents values of an 
      independent group of healthy patients around 30 years old. The present study 
      established a clinically similar T-score standard to diagnose osteoporosis in a 
      sheep model. MATERIAL AND METHODS We used 31 female merino land sheep (average 
      5.5 years old) to study osteoporosis. The following groups were compared using 
      DXA measurement: 1) control; 2) ovariectomized (OVX); 3) OVX combined with a 
      deficient diet (OVXD); and 4) OVXD combined with methylprednisolone 
      administration (OVXDS). Further, an independent group of 32 healthy sheep (4-6 
      years old) were measured as an independent baseline. BMD was measured at 0 
      months, 3 months, and 8 months after treatment. RESULTS The same significance 
      pattern between the treated groups and either baseline groups was seen. However, 
      using an independent baseline changed the "clinical" interpretation of the data 
      from an osteoporotic bone status (T-score <-2.5) after 3 months of OXDS treatment 
      into an osteopenic bone status (T-score <-1.5 to -2.4). CONCLUSIONS Using an 
      independent baseline enhanced the statistical significance and showed the 
      clinical relevance. Furthermore, an independent baseline is a reliable 
      alternative to use of a new control group for future experiments and thus reduces 
      the number of animals needed by eliminating the need for a control and 
      corresponding to clinical practice.
FAU - Heiss, Christian
AU  - Heiss C
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Kern, Stefanie
AU  - Kern S
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Malhan, Deeksha
AU  - Malhan D
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Bocker, Wolfgang
AU  - Bocker W
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
AD  - Department of General, Trauma, and Reconstructive Surgery, Ludwig-Maximilians 
      University, Munich, Germany.
FAU - Engelhardt, Markus
AU  - Engelhardt M
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
AD  - Department of Trauma, Hand, and Reconstructive Surgery, University Hospital of 
      Giessen-Marburg, Giessen, Germany.
FAU - Daghma, Diaa Eldin S
AU  - Daghma DES
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Stoetzel, Sabine
AU  - Stoetzel S
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Schmitt, Jakob
AU  - Schmitt J
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Ivo, Matthias
AU  - Ivo M
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Kauschke, Vivien
AU  - Kauschke V
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Lips, Katrin S
AU  - Lips KS
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
FAU - Tushtev, Kamen
AU  - Tushtev K
AD  - Advanced Ceramics, University of Bremen, Bremen, Germany.
FAU - Rezwan, Kurosch
AU  - Rezwan K
AD  - Advanced Ceramics, University of Bremen, Bremen, Germany.
FAU - El Khassawna, Thaqif
AU  - El Khassawna T
AD  - Department of Experimental Trauma Surgery, Faculty of Medicine, Justus-Liebig 
      University, Giessen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20171002
PL  - United States
TA  - Med Sci Monit Basic Res
JT  - Medical science monitor basic research
JID - 101597444
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Animals
MH  - Bone Density/physiology
MH  - Bone and Bones/*diagnostic imaging
MH  - Disease Models, Animal
MH  - Female
MH  - Methylprednisolone/pharmacology
MH  - Osteoporosis/*diagnosis
MH  - Ovariectomy/methods
MH  - Sheep
PMC - PMC5637575
COIS- Conflict of interest None.
EDAT- 2017/10/03 06:00
MHDA- 2018/07/06 06:00
PMCR- 2017/10/02
CRDT- 2017/10/03 06:00
PHST- 2017/10/03 06:00 [entrez]
PHST- 2017/10/03 06:00 [pubmed]
PHST- 2018/07/06 06:00 [medline]
PHST- 2017/10/02 00:00 [pmc-release]
AID - 905561 [pii]
AID - 10.12659/msmbr.905561 [doi]
PST - epublish
SO  - Med Sci Monit Basic Res. 2017 Oct 2;23:326-335. doi: 10.12659/msmbr.905561.