PMID- 28967606
OWN - NLM
STAT- MEDLINE
DCOM- 20180831
LR  - 20181202
IS  - 1953-8022 (Electronic)
IS  - 1246-7820 (Linking)
VI  - 25
IP  - 1
DP  - 2018 Feb
TI  - Differentiation of human dendritic cell subsets for immune tolerance induction.
PG  - 90-95
LID - S1246-7820(17)30490-1 [pii]
LID - 10.1016/j.tracli.2017.08.002 [doi]
AB  - OBJECTIVES: Since no further progress was achieved, in order to improve the 
      long-term organ transplantation outcome, the immune tolerance appears as an 
      interesting therapeutic goal. Dendritic cells (DCs) are specialized cells 
      participating in the homeostasis of the immune response. Moreover, subsets of 
      DCs, identified in humans, appear to have their respective competences in immune 
      response modulation. Our objective is to purify from PBMC or to differentiate DC 
      subsets from monocytes using several strategies and evaluate their IL10 
      secretion. METHODS: CD14(+) cells were purified from peripheral blood mononuclear 
      cell (PBMC) by affinity beads and cultured with cytokines up to 7 days. The pDCs 
      were purified with anti-BDCA-2 beads from PBMC fraction enriched by Percoll((R)) 
      gradient. The moDCs, pDCs and moLCs subsets were analyzed by phenotype labelling 
      and FACS analyses and IL10 secretion measured by ELISA. RESULTS: The moDCs were 
      characterized by the CD209 expression and a lower expression of CD1a markers. 
      Expression of CD207 and CD1a markers characterized moLCs and CD123(+)/BDCA-2(+) 
      pDCs. Variable IL-10 secretions were shown between the three DC subsets, both at 
      basal and activated levels. CONCLUSIONS: As the several DC populations studied 
      have different capacities of IL-10 synthesis, they might play, among others, 
      distinct roles in the induction of immune tolerance.
CI  - Copyright (c) 2017 Elsevier Masson SAS. All rights reserved.
FAU - Deluce-Kakwata-Nkor, N
AU  - Deluce-Kakwata-Nkor N
AD  - EA 4245 cellules dendritiques, immuno-modulation et greffes, universite 
      Francois-Rabelais de Tours, UFR de medecine, 10, boulevard Tonnelle, 37032 Tours 
      cedex, France.
FAU - Lamendour, L
AU  - Lamendour L
AD  - EA 4245 cellules dendritiques, immuno-modulation et greffes, universite 
      Francois-Rabelais de Tours, UFR de medecine, 10, boulevard Tonnelle, 37032 Tours 
      cedex, France.
FAU - Chabot, V
AU  - Chabot V
AD  - Service recherche du laboratoire d'histocompatibilite et d'immunogenetique, 
      etablissement francais du sang Centre-Atlantique, 2, boulevard Tonnelle, BP 
      40661, 37206 Tours cedex 3, France.
FAU - Heraud, A
AU  - Heraud A
AD  - EA 4245 cellules dendritiques, immuno-modulation et greffes, universite 
      Francois-Rabelais de Tours, UFR de medecine, 10, boulevard Tonnelle, 37032 Tours 
      cedex, France.
FAU - Ivanovic, Z
AU  - Ivanovic Z
AD  - EFS Aquitaine-Limousin, place Amelie-Raba-Leon, CS 21010, 33075 Bordeaux, France.
FAU - Halary, F
AU  - Halary F
AD  - UMR 1064, Inserm, centre de recherche en transplantation et immunologie (CRTI), 
      universite de Nantes, Nantes, France; Institut de transplantation urologie 
      nephrologie (ITUN), Hotel-Dieu, CHU de Nantes, 30, boulevard Jean-Monnet, 44093 
      Nantes cedex 01, France.
FAU - Dehaut, F
AU  - Dehaut F
AD  - Service recherche du laboratoire d'histocompatibilite et d'immunogenetique, 
      etablissement francais du sang Centre-Atlantique, 2, boulevard Tonnelle, BP 
      40661, 37206 Tours cedex 3, France.
FAU - Velge-Roussel, F
AU  - Velge-Roussel F
AD  - EA 4245 cellules dendritiques, immuno-modulation et greffes, universite 
      Francois-Rabelais de Tours, UFR de medecine, 10, boulevard Tonnelle, 37032 Tours 
      cedex, France; UFR des sciences pharmaceutiques, avenue Monge, 37000 Tours, 
      France. Electronic address: velge@univ-tours.fr.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20170928
PL  - France
TA  - Transfus Clin Biol
JT  - Transfusion clinique et biologique : journal de la Societe francaise de 
      transfusion sanguine
JID - 9423846
RN  - 0 (Antigens, CD)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (IL10 protein, human)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Cell Adhesion Molecules/analysis
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Cytapheresis
MH  - Dendritic Cells/classification/cytology/*immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry/methods
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - *Immune Tolerance
MH  - Immunomagnetic Separation/methods
MH  - Interleukin-10/metabolism
MH  - Interleukin-4/pharmacology
MH  - Lectins, C-Type/analysis
MH  - Monocytes/cytology
MH  - Receptors, Cell Surface/analysis
OTO - NOTNLM
OT  - Cellules dendritiques
OT  - DC subsets
OT  - Dendritic cells
OT  - IL-10
OT  - Immuno-modulation
OT  - Immunomodulation
OT  - Sous-types de DCs
EDAT- 2017/10/03 06:00
MHDA- 2018/09/01 06:00
CRDT- 2017/10/03 06:00
PHST- 2017/06/27 00:00 [received]
PHST- 2017/08/25 00:00 [accepted]
PHST- 2017/10/03 06:00 [pubmed]
PHST- 2018/09/01 06:00 [medline]
PHST- 2017/10/03 06:00 [entrez]
AID - S1246-7820(17)30490-1 [pii]
AID - 10.1016/j.tracli.2017.08.002 [doi]
PST - ppublish
SO  - Transfus Clin Biol. 2018 Feb;25(1):90-95. doi: 10.1016/j.tracli.2017.08.002. Epub 
      2017 Sep 28.