PMID- 28967682 OWN - NLM STAT- MEDLINE DCOM- 20171122 LR - 20171128 IS - 1099-0844 (Electronic) IS - 0263-6484 (Linking) VI - 35 IP - 7 DP - 2017 Oct TI - Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain-derived neurotrophic factor. PG - 364-371 LID - 10.1002/cbf.3283 [doi] AB - Brain-derived neurotrophic factor (BDNF) is a neurotrophin that can promote the development and proliferation of neurons. BDNF has been found to be involved in male reproduction. Leydig cells in testicular interstitial tissues can secrete testosterone in a luteinizing hormone-dependent manner. We showed that BDNF and its receptor TrkB were expressed in mice TM3 Leydig cells in the present study. Furthermore, BDNF can promote proliferation of mouse TM3 Leydig cells in vitro. Results of microRNA (miRNA) deep sequencing showed that BDNF can alter the expression profile of miRNAs in TM3 Leydig cells. Eighty-three miRNAs were significantly different in the BDNF-treated and control groups (fold change of >2.0 or <0.5, P < 0.05) wherein 40 were upregulated and 43 were downregulated. The expression levels of miR-125a-5p, miR-22-5p, miR-342-59, miR-451a, miR-148a-5p, miR-29b-3p, miR-199b-5p, and miR-145a-5p were further confirmed by quantitative real-time polymerase chain reaction. Bioinformatic analysis revealed that miRNAs regulated a large number of genes with different functions. Pathway analysis indicated that miRNAs participate in the pathways involved in signal transduction, cancer, metabolism, endocrine system, immune system, and nerve system. This study indicated that miRNAs might be involved in the BDNF-regulated cellular functions of Leydig cells. CI - Copyright (c) 2017 John Wiley & Sons, Ltd. FAU - Gao, Shan AU - Gao S AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Li, Chunjin AU - Li C AUID- ORCID: 0000-0002-4601-0488 AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Xu, Ying AU - Xu Y AD - Reproductive Medical Center, The Second Hospital of Jilin University, Changchun, China. FAU - Chen, Shuxiong AU - Chen S AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Zhao, Yun AU - Zhao Y AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Chen, Lu AU - Chen L AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Jiang, Yanwen AU - Jiang Y AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Liu, Zhuo AU - Liu Z AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Fan, Rong AU - Fan R AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Sun, Liting AU - Sun L AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Wang, Fengge AU - Wang F AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Zhu, Xiaoling AU - Zhu X AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Zhang, Jing AU - Zhang J AD - College of Animal Sciences, Jilin University, Jilin, China. FAU - Zhou, Xu AU - Zhou X AD - College of Animal Sciences, Jilin University, Jilin, China. LA - eng PT - Journal Article DEP - 20171002 PL - England TA - Cell Biochem Funct JT - Cell biochemistry and function JID - 8305874 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (MicroRNAs) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism/pharmacology MH - Cell Line MH - Cell Proliferation/drug effects MH - Computational Biology MH - Down-Regulation/drug effects MH - High-Throughput Nucleotide Sequencing MH - Leydig Cells/cytology/drug effects/metabolism MH - Male MH - Mice MH - MicroRNAs/chemistry/genetics/*metabolism MH - Microscopy, Fluorescence MH - Real-Time Polymerase Chain Reaction MH - Receptor, trkB/genetics/metabolism MH - Sequence Analysis, RNA MH - Signal Transduction/drug effects MH - Up-Regulation/drug effects OTO - NOTNLM OT - BDNF OT - TM3 Leydig cells OT - microRNAs EDAT- 2017/10/03 06:00 MHDA- 2017/11/29 06:00 CRDT- 2017/10/03 06:00 PHST- 2016/12/23 00:00 [received] PHST- 2017/03/14 00:00 [revised] PHST- 2017/07/07 00:00 [accepted] PHST- 2017/10/03 06:00 [pubmed] PHST- 2017/11/29 06:00 [medline] PHST- 2017/10/03 06:00 [entrez] AID - 10.1002/cbf.3283 [doi] PST - ppublish SO - Cell Biochem Funct. 2017 Oct;35(7):364-371. doi: 10.1002/cbf.3283. Epub 2017 Oct 2.