PMID- 28972400
OWN - NLM
STAT- MEDLINE
DCOM- 20171219
LR  - 20171219
IS  - 1557-7430 (Electronic)
IS  - 1044-5498 (Linking)
VI  - 36
IP  - 12
DP  - 2017 Dec
TI  - Knockdown of miR-629 Inhibits Ovarian Cancer Malignant Behaviors by Targeting 
      Testis-Specific Y-Like Protein 5.
PG  - 1108-1116
LID - 10.1089/dna.2017.3904 [doi]
AB  - Ovarian cancer (OC) is the most lethal gynecological cancer. The molecular 
      mechanism of it is complicated, and numerous researches suggest that microRNAs 
      are key regulators for it. This study was to investigate the pivotal role of 
      miR-629 in the progression of OC and to reveal the possible molecular mechanism 
      of its action. Testis-specific Y-like protein 5 (TSPYL5) is a tumor suppressor 
      gene in various cancers, but there is little for its role in OC. OC OVCAR3 cells 
      were transfected with the miR-629 vector, miR-629 inhibitor, and/or small 
      interfering RNA (siRNA) targeting TSPYL5 (si-TSPYL5), respectively. After 
      transfection, cell apoptosis, the ability of migration, and invasion were 
      explored, as well as the level of miR-629 and TSPYL5 protein expression were 
      detected by quantitative polymerase chain reaction and western blot. Compared 
      with the control, there was increasing of miR-629, and decreasing of TSPYL5 and 
      caspase 3 in OC tissue. Overexpression of miR-629 promoted the cell ability of 
      migration and invasion and reduced OC cell apoptosis. In addition, elevated 
      cancer inhibition ability of TSPYL5 induced by the miR-629 inhibitor was 
      significantly blocked by inhibition of TSPYL5 (si-TSPYL5). All the above results 
      suggested that miR-629 could promote OC proliferation, migration, and invasion by 
      directly suppressing TSPYL5 expression, and inhibition of miR-629 might serve as 
      a therapeutic target for OC.
FAU - Shao, Liping
AU  - Shao L
AD  - 1 Department of Obstetrics and Gynecology, The First Affiliated Hospital of 
      Soochow University , Suzhou, China .
AD  - 2 Department of Obstetrics and Gynecology, The People's Hospital of Suzhou 
      National New & Hi-Tech Development Zone , Suzhou, China .
AD  - 3 Department of Obstetrics and Gynecology, Taixing People's Hospital , Taizhou, 
      China .
FAU - Shen, Zongji
AU  - Shen Z
AD  - 1 Department of Obstetrics and Gynecology, The First Affiliated Hospital of 
      Soochow University , Suzhou, China .
FAU - Qian, Hua
AU  - Qian H
AD  - 3 Department of Obstetrics and Gynecology, Taixing People's Hospital , Taizhou, 
      China .
FAU - Zhou, Sufang
AU  - Zhou S
AD  - 2 Department of Obstetrics and Gynecology, The People's Hospital of Suzhou 
      National New & Hi-Tech Development Zone , Suzhou, China .
FAU - Chen, Youguo
AU  - Chen Y
AD  - 1 Department of Obstetrics and Gynecology, The First Affiliated Hospital of 
      Soochow University , Suzhou, China .
LA  - eng
PT  - Journal Article
DEP - 20171003
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (MIRN629 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (TSPYL5 protein, human)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Female
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Male
MH  - MicroRNAs/*antagonists & inhibitors/*genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics
MH  - Nuclear Proteins/*antagonists & inhibitors/*genetics/metabolism
MH  - Ovarian Neoplasms/*genetics/metabolism/pathology
MH  - RNA, Neoplasm/antagonists & inhibitors/genetics/metabolism
MH  - RNA, Small Interfering/genetics
OTO - NOTNLM
OT  - TSPYL5
OT  - cell proliferation
OT  - miR-629
OT  - migration and invasion
OT  - ovarian cancer
EDAT- 2017/10/04 06:00
MHDA- 2017/12/20 06:00
CRDT- 2017/10/04 06:00
PHST- 2017/10/04 06:00 [pubmed]
PHST- 2017/12/20 06:00 [medline]
PHST- 2017/10/04 06:00 [entrez]
AID - 10.1089/dna.2017.3904 [doi]
PST - ppublish
SO  - DNA Cell Biol. 2017 Dec;36(12):1108-1116. doi: 10.1089/dna.2017.3904. Epub 2017 
      Oct 3.