PMID- 28973322
OWN - NLM
STAT- MEDLINE
DCOM- 20171006
LR  - 20211028
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 58
IP  - 11
DP  - 2017 Sep 1
TI  - Intraretinal Correlates of Reticular Pseudodrusen Revealed by Autofluorescence 
      and En Face OCT.
PG  - 4769-4777
LID - 10.1167/iovs.17-22338 [doi]
AB  - PURPOSE: We sought to determine whether information revealed from the 
      reflectance, autofluorescence, and absorption properties of RPE cells situated 
      posterior to reticular pseudodrusen (RPD) could provide insight into the origins 
      and structure of RPD. METHODS: RPD were studied qualitatively by near-infrared 
      fundus autofluorescence (NIR-AF), short-wavelength fundus autofluorescence 
      (SW-AF), and infrared reflectance (IR-R) images, and the presentation was 
      compared to horizontal and en face spectral domain optical coherence tomographic 
      (SD-OCT) images. Images were acquired from 23 patients (39 eyes) diagnosed with 
      RPD (mean age 80.7 +/- 7.1 [SD]; 16 female; 4 Hispanics, 19 non-Hispanic whites). 
      RESULTS: In SW-AF, NIR-AF, and IR-R images, fundus RPD were recognized as 
      interlacing networks of small scale variations in IR-R and fluorescence (SW-AF, 
      NIR-AF) intensities. Darkened foci of RPD colocalized in SW-AF and NIR-AF images, 
      and in SD-OCT images corresponded to disturbances of the interdigitation (IZ) and 
      ellipsoid (EZ) zones and to more pronounced hyperreflective lesions traversing 
      photoreceptor-attributable bands in SD-OCT images. Qualitative assessment of the 
      outer nuclear layer (ONL) revealed thinning as RPD extended radially from the 
      outer to inner retina. In en face OCT, hyperreflective areas in the EZ band 
      correlated topographically with hyporeflective foci at the level of the RPE. 
      CONCLUSIONS: The hyperreflective lesions corresponding to RPD in SD-OCT scans are 
      likely indicative of degenerating photoreceptor cells. The darkened foci at 
      positions of RPD in NIR-AF and en face OCT images indicate changes in the RPE 
      monolayer with the reduced NIR-AF and en face OCT signal suggesting a reduction 
      in melanin that could be accounted for by RPE thinning.
FAU - Paavo, Maarjaliis
AU  - Paavo M
AD  - Department of Ophthalmology, Columbia University Medical Center, New York, New 
      York, United States.
FAU - Lee, Winston
AU  - Lee W
AD  - Department of Ophthalmology, Columbia University Medical Center, New York, New 
      York, United States.
FAU - Merriam, John
AU  - Merriam J
AD  - Department of Ophthalmology, Columbia University Medical Center, New York, New 
      York, United States.
FAU - Bearelly, Srilaxmi
AU  - Bearelly S
AD  - Department of Ophthalmology, Columbia University Medical Center, New York, New 
      York, United States.
FAU - Tsang, Stephen
AU  - Tsang S
AD  - Department of Ophthalmology, Columbia University Medical Center, New York, New 
      York, United States.
AD  - Department of Pathology and Cell Biology, Columbia University Medical Center, New 
      York, New York, United States.
FAU - Chang, Stanley
AU  - Chang S
AD  - Department of Ophthalmology, Columbia University Medical Center, New York, New 
      York, United States.
FAU - Sparrow, Janet R
AU  - Sparrow JR
AD  - Department of Ophthalmology, Columbia University Medical Center, New York, New 
      York, United States.
AD  - Department of Pathology and Cell Biology, Columbia University Medical Center, New 
      York, New York, United States.
LA  - eng
GR  - P30 EY019007/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
SB  - IM
CIN - Invest Ophthalmol Vis Sci. 2017 Dec 1;58(14 ):6193. PMID: 29222546
CIN - Invest Ophthalmol Vis Sci. 2017 Dec 1;58(14 ):6194. PMID: 29222547
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Fluorescein Angiography/*methods
MH  - Fundus Oculi
MH  - Humans
MH  - Macular Degeneration/*diagnostic imaging
MH  - Male
MH  - Middle Aged
MH  - Optical Imaging/methods
MH  - Retinal Drusen/*diagnostic imaging
MH  - Retinal Pigment Epithelium/*diagnostic imaging
MH  - Tomography, Optical Coherence/*methods
PMC - PMC5624777
EDAT- 2017/10/04 06:00
MHDA- 2017/10/07 06:00
PMCR- 2017/10/01
CRDT- 2017/10/04 06:00
PHST- 2017/10/04 06:00 [entrez]
PHST- 2017/10/04 06:00 [pubmed]
PHST- 2017/10/07 06:00 [medline]
PHST- 2017/10/01 00:00 [pmc-release]
AID - 2655024 [pii]
AID - IOVS-17-22338 [pii]
AID - 10.1167/iovs.17-22338 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2017 Sep 1;58(11):4769-4777. doi: 
      10.1167/iovs.17-22338.