PMID- 28975377
OWN - NLM
STAT- MEDLINE
DCOM- 20181123
LR  - 20181123
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 409
IP  - 29
DP  - 2017 Nov
TI  - Upconversion fluorescence immunoassay for imidaclothiz by magnetic nanoparticle 
      separation.
PG  - 6885-6892
LID - 10.1007/s00216-017-0653-7 [doi]
AB  - A sensitive fluorescence immunoassay for the detection of imidaclothiz was 
      established by using magnetic nanoparticles (MNPs) as concentration elements and 
      upconversion nanoparticles (UCNPs) as signal labels. The NaYF(4)/Yb,Er UCNPs and 
      MNPs were conjugated with imidaclothiz monoclonal antibody and imidaclothiz 
      antigen, respectively. Imidaclothiz could compete with the antigen-conjugated 
      MNPs for binding to the antibody-conjugated UCNPs and resulted in a decreased 
      fluorescence signal when the MNPs were separated by an external magnet. Under the 
      optimal conditions, the concentration of imidaclothiz producing 50% inhibition of 
      the signal (IC(50)), limit of detection (LOD, IC(10)), and the linear assay range 
      (IC(10)-IC(90)) were 14.59, 0.74, and 0.74-289.30 ng mL(-1), respectively. The 
      immunoassay exhibited no obvious cross-reactivity with analogues of imidaclothiz 
      except for imidacloprid, with 89.2% cross-reactivity. The average recoveries 
      measured in paddy water, pear, soil, peach, rice, tomato, wheat, and pakchoi were 
      75.7-105.2%, and the relative standard deviations (RSDs) were less than 11.2%. In 
      addition, the results of the immunoassay correlated well with that of 
      high-performance liquid chromatography (HPLC) for authentic samples. Graphical 
      abstract Development of an upconversion fluorescence immunoassay for the 
      detection of imidaclothiz by using antibody-modified upconversion nanoparticles 
      (UCNPs) as the detection probe and antigen-modified magnetic nanoparticles (MNPs) 
      as the capture probe.
FAU - Hua, Xiude
AU  - Hua X
AD  - College of Plant Protection (State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing, 210095, China.
FAU - You, Hongjie
AU  - You H
AD  - College of Plant Protection (State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing, 210095, China.
FAU - Luo, Peiwen
AU  - Luo P
AD  - College of Plant Protection (State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing, 210095, China.
FAU - Tao, Zhexuan
AU  - Tao Z
AD  - College of Plant Protection (State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing, 210095, China.
FAU - Chen, He
AU  - Chen H
AD  - College of Plant Protection (State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing, 210095, China.
FAU - Liu, Fengquan
AU  - Liu F
AD  - College of Plant Protection (State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing, 210095, China.
AD  - Institute of Plant Protection, Jiangsu Academy of Agricultural Science, Nanjing, 
      210014, China.
FAU - Wang, Minghua
AU  - Wang M
AD  - College of Plant Protection (State & Local Joint Engineering Research Center of 
      Green Pesticide Invention and Application), Nanjing Agricultural University, 
      Nanjing, 210095, China. wangmha@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20171003
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Thiazoles)
RN  - E070T0J4TU (imidaclothiz)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Fluorescence
MH  - *Fluorescent Antibody Technique
MH  - Immunoassay/*methods
MH  - Limit of Detection
MH  - Magnetics
MH  - Magnetite Nanoparticles/*chemistry
MH  - Thiazoles/*analysis/chemistry
OTO - NOTNLM
OT  - Imidaclothiz
OT  - Immunoassay
OT  - Magnetic nanoparticles (MNPs)
OT  - Upconversion nanoparticles (UCNPs)
EDAT- 2017/10/05 06:00
MHDA- 2018/11/24 06:00
CRDT- 2017/10/05 06:00
PHST- 2017/07/10 00:00 [received]
PHST- 2017/09/18 00:00 [accepted]
PHST- 2017/09/12 00:00 [revised]
PHST- 2017/10/05 06:00 [pubmed]
PHST- 2018/11/24 06:00 [medline]
PHST- 2017/10/05 06:00 [entrez]
AID - 10.1007/s00216-017-0653-7 [pii]
AID - 10.1007/s00216-017-0653-7 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2017 Nov;409(29):6885-6892. doi: 10.1007/s00216-017-0653-7. 
      Epub 2017 Oct 3.