PMID- 28976943 OWN - NLM STAT- MEDLINE DCOM- 20180615 LR - 20190606 IS - 1643-3750 (Electronic) IS - 1234-1010 (Print) IS - 1234-1010 (Linking) VI - 23 DP - 2017 Oct 4 TI - Essential Oil from Fructus Alpiniae Zerumbet Protects Human Umbilical Vein Endothelial Cells In Vitro from Injury Induced by High Glucose Levels by Suppressing Nuclear Transcription Factor-Kappa B Signaling. PG - 4760-4767 AB - BACKGROUND In China, the essential oil of the fruit, Fructus Alpiniae zerumbet (FAZ), is used to treat cardiovascular diseases. Recent in vitro studies have shown that the essential oil of FAZ (EOFAZ) can protect endothelial cells from injury. Because of the prevalence of diabetes mellitus and its effects on the cardiovascular system, the aim of this study was to investigate the mechanism of the effects of EOFAZ on human umbilical vein endothelial cells (HUVECs) treated with high levels of glucose in vitro. MATERIAL AND METHODS The lactate dehydrogenase (LDH) leakage assay was used to detect HUVEC injury. Tumor necrosis factor-alpha (TNF-alpha), interleukin-8 (IL-8), and nuclear transcription factor-kappa B (NF-kappaB) p65 subunit DNA-binding activity was detected. The expression of NF-kappaB pathway-associated proteins, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) was studied by Western blotting. The cellular location of NF-kappaB in HUVECs was evaluated using immunofluorescence. RESULTS Cell viability and LDH leakage assays showed that high glucose-induced HUVEC injury was reduced by EOFAZ. High glucose-induced secretion of IL-8, TNF-alpha, ICAM-1, and VCAM-1 was reduced, and translocation of the p65 subunit of NF-kappaB to the endothelial cell nucleus was inhibited by EOFAZ. Western blotting confirmed that EOFAZ blocked the activation of NF-kappaB induced by high glucose levels. EOFAZ reduced high glucose-induced p65/DNA binding to inhibit NF-kappaB activation. CONCLUSIONS The findings of this in vitro study showed that treatment of HUVECs with EOFAZ had a protective role against the effects of high glucose levels via the NF-kappaB signaling pathway. FAU - Huang, Niwen AU - Huang N AD - Department of Cardiovascular Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland). AD - The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Drugability, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). AD - Department of Respiratory Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland). FAU - Xu, Yini AU - Xu Y AD - The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Drugability, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). AD - The Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). FAU - Zhou, Haiyan AU - Zhou H AD - Department of Cardiovascular Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland). FAU - Lin, Dan AU - Lin D AD - The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Drugability, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). AD - The Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). FAU - Zhang, Bei AU - Zhang B AD - Department of Respiratory Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland). FAU - Zhang, Yanyan AU - Zhang Y AD - The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Drugability, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). AD - The Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). FAU - Pan, Di AU - Pan D AD - The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Drugability, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). AD - The Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). FAU - Tao, Ling AU - Tao L AD - The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Drugability, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). AD - The Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). FAU - Liu, Xingde AU - Liu X AD - Department of Cardiovascular Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland). AD - Department of Respiratory Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China (mainland). AD - The Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). FAU - Shen, Xiangchun AU - Shen X AD - The High Educational Key Laboratory of Guizhou Province for Natural Medicinal Pharmacology and Drugability, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). AD - The Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Huaxi University Town, Guiyang, Guizhou, China (mainland). LA - eng PT - Journal Article DEP - 20171004 PL - United States TA - Med Sci Monit JT - Medical science monitor : international medical journal of experimental and clinical research JID - 9609063 RN - 0 (I-kappa B Proteins) RN - 0 (NF-kappa B) RN - 0 (Oils, Volatile) RN - 0 (Protective Agents) RN - 0 (Transcription Factor RelA) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (Vascular Cell Adhesion Molecule-1) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) RN - IY9XDZ35W2 (Glucose) SB - IM MH - Alpinia/*metabolism MH - Cells, Cultured MH - China MH - Endothelium, Vascular/drug effects MH - Fruit MH - Gene Expression Regulation/drug effects MH - Glucose/*adverse effects/metabolism MH - Human Umbilical Vein Endothelial Cells/*drug effects MH - Humans MH - I-kappa B Proteins/metabolism MH - Intercellular Adhesion Molecule-1/biosynthesis MH - Medicine, Chinese Traditional MH - NF-kappa B/metabolism MH - Oils, Volatile/pharmacology MH - Protective Agents/pharmacology MH - Signal Transduction/drug effects MH - Transcription Factor RelA/metabolism MH - Tumor Necrosis Factor-alpha/metabolism MH - Vascular Cell Adhesion Molecule-1/biosynthesis PMC - PMC5637625 COIS- Conflicts of interests None. EDAT- 2017/10/05 06:00 MHDA- 2018/06/16 06:00 PMCR- 2017/10/04 CRDT- 2017/10/05 06:00 PHST- 2017/10/05 06:00 [entrez] PHST- 2017/10/05 06:00 [pubmed] PHST- 2018/06/16 06:00 [medline] PHST- 2017/10/04 00:00 [pmc-release] AID - 906463 [pii] AID - 10.12659/msm.906463 [doi] PST - epublish SO - Med Sci Monit. 2017 Oct 4;23:4760-4767. doi: 10.12659/msm.906463.