PMID- 28979192
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 11
DP  - 2017
TI  - Arc Interacts with the Integral Endoplasmic Reticulum Protein, Calnexin.
PG  - 294
LID - 10.3389/fncel.2017.00294 [doi]
LID - 294
AB  - Activity-regulated cytoskeleton-associated protein, Arc, is a major regulator of 
      long-term synaptic plasticity and memory formation. Here we reveal a novel 
      interaction partner of Arc, a resident endoplasmic reticulum transmembrane 
      protein, calnexin. We show an interaction between recombinantly-expressed 
      GST-tagged Arc and endogenous calnexin in HEK293, SH-SY5Y neuroblastoma and PC12 
      cells. The interaction was dependent on the central linker region of the Arc 
      protein that is also required for endocytosis of AMPA-type glutamate receptors. 
      High-resolution proximity-ligation assays (PLAs) demonstrate molecular proximity 
      of endogenous Arc with the cytosolic C-terminus, but not the lumenal N-terminus 
      of calnexin. In hippocampal neuronal cultures treated with brain-derived 
      neurotrophic factor (BDNF), Arc interacted with calnexin in the perinuclear 
      cytoplasm and dendritic shaft. Arc also interacted with C-terminal calnexin in 
      the adult rat dentate gyrus (DG). After induction of long-term potentiation (LTP) 
      in the perforant path projection to the DG of adult anesthetized rats, enhanced 
      interaction between Arc and calnexin was obtained in the dentate granule cell 
      layer (GCL). Although Arc and calnexin are both implicated in the regulation of 
      receptor endocytosis, no modulation of endocytosis was detected in transferrin 
      uptake assays. Previous work showed that Arc interacts with multiple protein 
      partners to regulate synaptic transmission and nuclear signaling. The 
      identification of calnexin as a binding partner further supports the role of Arc 
      as a hub protein and extends the range of Arc function to the endoplasmic 
      reticulum, though the function of the Arc/calnexin interaction remains to be 
      defined.
FAU - Myrum, Craig
AU  - Myrum C
AD  - Dr. Einar Martens Research Group for Biological Psychiatry, Center for Medical 
      Genetics and Molecular Medicine, Haukeland University HospitalBergen, Norway.
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Soule, Jonathan
AU  - Soule J
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Bittins, Margarethe
AU  - Bittins M
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Cavagnini, Kyle
AU  - Cavagnini K
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Goff, Kevin
AU  - Goff K
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Ziemek, Silje K
AU  - Ziemek SK
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Eriksen, Maria S
AU  - Eriksen MS
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Patil, Sudarshan
AU  - Patil S
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Szum, Adrian
AU  - Szum A
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Nair, Rajeevkumar R
AU  - Nair RR
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
FAU - Bramham, Clive R
AU  - Bramham CR
AD  - Department of Biomedicine and the K.G. Jebsen Center for Research on 
      Neuropsychiatric Disorders, University of BergenBergen, Norway.
LA  - eng
PT  - Journal Article
DEP - 20170920
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC5611444
OTO - NOTNLM
OT  - arc
OT  - calnexin
OT  - endocytosis
OT  - endoplasmic reticulum
OT  - proximity ligation assay
OT  - synaptic plasticity
EDAT- 2017/10/06 06:00
MHDA- 2017/10/06 06:01
PMCR- 2017/01/01
CRDT- 2017/10/06 06:00
PHST- 2017/07/12 00:00 [received]
PHST- 2017/09/05 00:00 [accepted]
PHST- 2017/10/06 06:00 [entrez]
PHST- 2017/10/06 06:00 [pubmed]
PHST- 2017/10/06 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2017.00294 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2017 Sep 20;11:294. doi: 10.3389/fncel.2017.00294. 
      eCollection 2017.