PMID- 28979326
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1735-0328 (Print)
IS  - 1726-6890 (Electronic)
IS  - 1726-6882 (Linking)
VI  - 16
IP  - 2
DP  - 2017 Spring
TI  - In-vitro Assessment of the Antiproliferative and Apoptotic Potential of the Ethyl 
      acetate Extract of Peltophorumafricanum on Different Cancer Cell Lines.
PG  - 714-724
AB  - We evaluated the in-vitro antiproliferative and apoptotic potential of the ethyl 
      acetate extract (EAE) of Peltophorum africanum, a member of the family Fabaceae 
      (Sond) in order to validate its pharmacological use. Antiproliferation of human 
      breast (MCF-7), colon (HT-29) and cervical (HeLa) cancer cell lines by EAE was 
      investigated using the Cell Titer-Blue viability assay and the mechanism of 
      action delineated using the Nucleic Acid and Protein Purification Nucleospin((R)) 
      Tissue Kit, Scanning Electron Microscope (SEM), propidium iodide (PI) and 
      acridine orange (AO) double-staining techniques. We observed a significant 
      reduction in cell viability of the MCF-7 cells from 100% (untreated) to 54.33 +/- 
      1.84% after 72 h of treatment with 5 microg/mL of EAE (P. value < 0.05). 
      Internucleosomal DNA of MCF-7, HT-29 and HeLa cells was randomly fragmented into 
      an uninterrupted spectrum of sizes, complemented by the intercalation of nucleic 
      acid-specific fluorochromes by PI and AO spotting two phases of apoptosis; early 
      (EA) and late (LA) apoptosis. Distinctive ultramorphological changes observed 
      included; cell shrinkage, membrane blebbing, and typical cell induced death. The 
      ethyl acetate extract of P.africanum has the potential to induce apoptosis and is 
      undergoing further studies in-vivo as a likely template for new anticancer 
      therapy.
FAU - Ifeoluwa Okeleye, Benjamin
AU  - Ifeoluwa Okeleye B
AD  - Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of 
      Biochemistry and Microbiology, Faculty of Science and Agriculture, University of 
      Fort Hare, South Africa.
AD  - Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, 
      University of the Free State, Qwaqwa Campus, Phuthaditjhaba 9866, South Africa.
FAU - Mkwetshana, Noxolo Thabiso
AU  - Mkwetshana NT
AD  - Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of 
      Biochemistry and Microbiology, Faculty of Science and Agriculture, University of 
      Fort Hare, South Africa.
FAU - Ndip, Roland Ndip
AU  - Ndip RN
AD  - Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of 
      Biochemistry and Microbiology, Faculty of Science and Agriculture, University of 
      Fort Hare, South Africa.
AD  - Department of Microbiology and Parasitology, Faculty of Science, University of 
      Buea, Box 63, Buea, Cameroon.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Iran J Pharm Res
JT  - Iranian journal of pharmaceutical research : IJPR
JID - 101208407
PMC - PMC5603881
OTO - NOTNLM
OT  - Antiproliferation
OT  - Chemotherapy
OT  - Medicinal plant
OT  - Peltophorum africanum
OT  - apoptosis
COIS- The authors declare no conflict of interests.
EDAT- 2017/10/06 06:00
MHDA- 2017/10/06 06:01
PMCR- 2017/03/01
CRDT- 2017/10/06 06:00
PHST- 2017/10/06 06:00 [entrez]
PHST- 2017/10/06 06:00 [pubmed]
PHST- 2017/10/06 06:01 [medline]
PHST- 2017/03/01 00:00 [pmc-release]
AID - ijpr-16-714 [pii]
PST - ppublish
SO  - Iran J Pharm Res. 2017 Spring;16(2):714-724.