PMID- 28980168
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR  - 20191210
IS  - 1573-7438 (Electronic)
IS  - 0049-4747 (Linking)
VI  - 50
IP  - 2
DP  - 2018 Feb
TI  - Oral fluid samples used for PRRSV acclimatization program and sow performance 
      monitoring in endemic PRRS-positive farms.
PG  - 291-298
LID - 10.1007/s11250-017-1428-z [doi]
AB  - An effective gilt acclimatization program is one of the most important management 
      strategies for controlling porcine reproductive and respiratory syndrome virus 
      (PRRSV) infection. Recently, oral fluid samples have been used as alternative 
      diagnostic samples for various swine diseases. This study utilized oral fluids 
      for PRRSV monitoring during the gilt acclimatization period in PRRSV endemic 
      farms. The study was performed in two selected commercial breeding herds (farm A 
      and farm B). PRRSV RNA and PRRSV-specific antibodies were monitored using oral 
      fluid and serum samples. Sow performance parameters related to PRRSV infection 
      were recorded and assessed. After PRRSV exposure during acclimatization, viral 
      RNA was demonstrated in oral fluids from 1 to 10 weeks post-exposure (WPE). PRRSV 
      RNA was detected in serum at 1 and 4 WPE in farm A and at 1, 4, 8, and 12 WPE in 
      farm B. Prolonged viremia of gilts from farm B was possibly due to re-infection 
      (within the herd) and later, reproductive problems were found in the breeding 
      herd. The correlation of PRRSV RNA concentration in oral fluids and serum was 
      evident. The S/P ratio values of PRRSV antibodies in oral fluid samples were 
      higher and had similar patterns of antibody responses to the serum samples. The 
      results suggest that the use of oral fluid samples for PRRSV monitoring during 
      gilt acclimatization in endemic farms is effective, convenient, practical, and 
      economical and would be most beneficial when used with other parameters.
FAU - Woonwong, Yonlayong
AU  - Woonwong Y
AD  - Graduate Program in Veterinary Pathobiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.
FAU - Kedkovid, Roongtham
AU  - Kedkovid R
AD  - Graduate Program in Veterinary Pathobiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.
FAU - Arunorat, Jirapat
AU  - Arunorat J
AD  - Graduate Program in Veterinary Pathobiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.
FAU - Sirisereewan, Chaitawat
AU  - Sirisereewan C
AD  - Graduate Program in Veterinary Pathobiology, Faculty of Veterinary Science, 
      Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.
FAU - Nedumpun, Teerawut
AU  - Nedumpun T
AD  - Inter-Department of Medical Microbiology, Graduate School, Chulalongkorn 
      University, Pathumwan, Bangkok, 10330, Thailand.
FAU - Poonsuk, Korakrit
AU  - Poonsuk K
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA.
FAU - Panyasing, Yaowalak
AU  - Panyasing Y
AD  - Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn 
      University, Pathumwan, Bangkok, 10330, Thailand.
FAU - Poolperm, Pariwat
AU  - Poolperm P
AD  - Department of Farm Resources and Production Medicine, Faculty of Veterinary 
      Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand.
FAU - Boonsoongnern, Alongkot
AU  - Boonsoongnern A
AD  - Department of Farm Resources and Production Medicine, Faculty of Veterinary 
      Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand. 
      fvetakb@ku.ac.th.
FAU - Thanawongnuwech, Roongroje
AU  - Thanawongnuwech R
AD  - Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn 
      University, Pathumwan, Bangkok, 10330, Thailand. roongroje.t@chula.ac.th.
LA  - eng
GR  - NRU-59-PPS023-HR/National Research University Project, Office of Higher Education 
      Commission/
PT  - Evaluation Study
PT  - Journal Article
DEP - 20171005
PL  - United States
TA  - Trop Anim Health Prod
JT  - Tropical animal health and production
JID - 1277355
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Acclimatization
MH  - Animals
MH  - Antibodies, Viral/*analysis
MH  - Antibody Formation
MH  - Farms
MH  - Female
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/immunology/*isolation & 
      purification
MH  - RNA, Viral/*analysis
MH  - Saliva/*virology
MH  - Swine
MH  - Swine Diseases
OTO - NOTNLM
OT  - Acclimatization
OT  - Gilts
OT  - Monitoring
OT  - Oral fluid
OT  - PRRSV
OT  - Reproductive performance
EDAT- 2017/10/06 06:00
MHDA- 2018/07/10 06:00
CRDT- 2017/10/06 06:00
PHST- 2017/02/01 00:00 [received]
PHST- 2017/09/20 00:00 [accepted]
PHST- 2017/10/06 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
PHST- 2017/10/06 06:00 [entrez]
AID - 10.1007/s11250-017-1428-z [pii]
AID - 10.1007/s11250-017-1428-z [doi]
PST - ppublish
SO  - Trop Anim Health Prod. 2018 Feb;50(2):291-298. doi: 10.1007/s11250-017-1428-z. 
      Epub 2017 Oct 5.