PMID- 28981944
OWN - NLM
STAT- MEDLINE
DCOM- 20180313
LR  - 20180313
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 34
IP  - 5
DP  - 2017 Oct 10
TI  - [Association of transcobalamine II gene polymorphisms and serum homocysteine, 
      vitamin B(12) and folate levels with ulcerative colitis among Chinese patients].
PG  - 731-736
LID - 10.3760/cma.j.issn.1003-9406.2017.05.025 [doi]
AB  - OBJECTIVE: To assess the association of transcobalamine II (TCN2) gene 
      polymorphisms and serum levels of homocysteine (Hcy), vitamin B(12) and folate 
      with ulcerative colitis (UC) among Chinese patients. METHODS: For 397 UC patients 
      and 574 controls, two single nucleotide polymorphisms of the TCN2 gene 
      (rs1801198, rs9606756) were tested with an improved multiple ligase detection 
      reaction method. Serum Hcy, vitamin B(12) and folate were measured with an 
      enzymatic cycling assay and an chemiluminescence immunoassay, respectively. 
      RESULTS: The allelic and genotypic frequencies of rs1801198 and rs9606756 did not 
      differ significantly between the two groups (all P> 0.05). Compared with those of 
      the control group, the frequencies of G allele and CG+GG genotype of rs1801198 
      were greater in patients with moderate and severe UC (both P< 0.05). The same 
      conclusion may also be drawn for the G allele and AG genotype of rs9606756 (both 
      P< 0.05). Compared with the controls, average Hcy level was enhanced in UC 
      patients (P< 0.01), whereas average vitamin B(12) and folate levels were 
      decreased in UC patients (both P< 0.01). In both groups, the average level of Hcy 
      was lower in individuals carrying CC of (rs1801198) than in those with CG+GG 
      (both P< 0.05). A similar conclusion was also drawn for individuals with AA of 
      rs9606756 when compared with those carrying AG(both P< 0.05). Compared with 
      patients with mild UC, average Hcy level was increased in those with moderate and 
      severe UC (P< 0.01), while average vitamin B(12) and folate levels were decreased 
      in those with moderate and severe UC (both P< 0.01). The prevalence of 
      hyperhomocysteinemia(HHcy), vitamin B(12) deficiency and folate deficiency was 
      greater in UC patients than in controls (all P< 0.01). In UC patients, the level 
      of Hcy was negatively correlated with those of vitamin B(12) (P< 0.01), 
      albumin(P< 0.01), red blood cells(P< 0.01) and platelet (P< 0.05), but positively 
      correlated with white blood cells(P< 0.01) and Mayo score (P< 0.01). Both HHcy 
      and folate deficiency were independent risk factors for UC (OR=4.173, OR=5.206, 
      both P< 0.01). CONCLUSION: TCN2 (rs1801198, rs9606756) variations, as well as 
      serum levels of Hcy, vitamin B(12) and folate, are correlated with UC. Both HHcy 
      and folate deficiency are independent risk factors for UC.
FAU - Zheng, Shuzi
AU  - Zheng S
AD  - Department of Gastroenterology, the Second Affiliated Hospital, Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China. wzjiangyi@yeah.net.
FAU - Wu, Hao
AU  - Wu H
FAU - Ye, Fangpeng
AU  - Ye F
FAU - Xia, Xuanping
AU  - Xia X
FAU - Xia, Shenglong
AU  - Xia S
FAU - Lin, Xiuqing
AU  - Lin X
FAU - Wu, Xiaoli
AU  - Wu X
FAU - Jiang, Lijia
AU  - Jiang L
FAU - Ding, Ran
AU  - Ding R
FAU - Jiang, Yi
AU  - Jiang Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Transcobalamins)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 935E97BOY8 (Folic Acid)
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Adult
MH  - Colitis, Ulcerative/blood/etiology/*genetics
MH  - Female
MH  - Folic Acid/*blood
MH  - Genotype
MH  - Homocysteine/*blood
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
MH  - Transcobalamins/*genetics
MH  - Vitamin B 12/*blood
EDAT- 2017/10/06 06:00
MHDA- 2018/03/14 06:00
CRDT- 2017/10/06 06:00
PHST- 2017/10/06 06:00 [entrez]
PHST- 2017/10/06 06:00 [pubmed]
PHST- 2018/03/14 06:00 [medline]
AID - 940634150 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2017.05.025 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2017 Oct 10;34(5):731-736. doi: 
      10.3760/cma.j.issn.1003-9406.2017.05.025.