PMID- 28982865
OWN - NLM
STAT- MEDLINE
DCOM- 20171016
LR  - 20220410
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 37
IP  - 10
DP  - 2017 Oct
TI  - Deregulation of PTEN Expression in Laryngeal Squamous Cell Carcinoma Based on 
      Tissue Microarray Digital Analysis.
PG  - 5521-5524
AB  - BACKGROUND/AIM: Phosphatase and tensin homolog (PTEN) (gene locus: 10q23.3) -a 
      tumor suppressor gene- is deleted, mutated or epigenetically hyper-methylated in 
      a variety of malignancies. PTEN acts as a negative regulator in PI3K/AKT/mTOR 
      signaling transduction pathway. Our aim was to investigate PTEN protein 
      expression patterns in laryngeal squamous cell carcinomas (LSCC). MATERIALS AND 
      METHODS: Using tissue microarray technology, fifty (n=50) primary LSCCs were 
      cored and re-embedded into one recipient block. Immunohistochemistry and digital 
      image analysis were implemented for evaluating protein expression levels. 
      RESULTS: Abnormal protein expression (low to negative staining intensity values) 
      was observed in 28/50 (56%) tissue cores. Overall PTEN expression was associated 
      with the anatomical region of the malignancies (p=0.039), whereas a borderline 
      correlation with the differentiation grade was also assessed (p=0.05). 
      CONCLUSION: Aberrant expression of PTEN tumor-suppressor gene in LSCCs seems to 
      affect their biological behavior. Well-differentiated tumors express moderate to 
      high protein levels, an evidence of normal gene function, whereas loss of its 
      expression correlates with a progressive tumor dedifferentiation. Additionally, 
      loss of its expression is detected more frequently in specific anatomical regions 
      of the larynx (glottis, predominantly, and partially supraglottis).
CI  - Copyright(c) 2017, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Mastronikolis, Nicholas S
AU  - Mastronikolis NS
AD  - ENT Department, Medical School, University of Patras, Patras, Greece.
FAU - Tsiambas, Evangelos
AU  - Tsiambas E
AD  - Department of Immunohistochemistry & Molecular Biology, 401 GAH, Athens, Greece 
      tsiambasecyto@yahoo.gr.
FAU - Papadas, Theodoros A
AU  - Papadas TA
AD  - ENT Department, Medical School, University of Patras, Patras, Greece.
FAU - Karameris, Andreas
AU  - Karameris A
AD  - Department of Pathology, 417 VA Hospital, Athens, Greece.
FAU - Ragos, Vasileios
AU  - Ragos V
AD  - Department of Maxillofacial, Medical School, University of Ioannina, Ioannina, 
      Greece.
FAU - Peschos, Dimitrios
AU  - Peschos D
AD  - Department of Physiology, Medical School, University of Ioannina, Ioannina, 
      Greece.
FAU - Mastronikolis, Stylianos N
AU  - Mastronikolis SN
AD  - Department of Medicine, School of Health Sciences, University of Crete, 
      Heraklion, Greece.
FAU - Papadas, Athanasios T
AU  - Papadas AT
AD  - Department of Hematology and Oncology, University of Wisconsin-Madison School of 
      Medicine, Madison, WI, U.S.A.
FAU - Liatsos, Christos
AU  - Liatsos C
AD  - Department of Gastroenterology, 401 GAH, Athens, Greece.
FAU - Armata, Ilianna E
AU  - Armata IE
AD  - Medical School, St. George's, University of London medical programme, University 
      of Nicosia, Nicosia, Cyprus.
FAU - Fotiades, Panagiotis P
AU  - Fotiades PP
AD  - Department of Surgery, Leicester General Hospital, Leicester, U.K.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Biomarkers, Tumor/*analysis
MH  - Carcinoma, Squamous Cell/*enzymology/pathology
MH  - Cell Dedifferentiation
MH  - Down-Regulation
MH  - Female
MH  - Head and Neck Neoplasms/*enzymology/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*enzymology/pathology
MH  - Male
MH  - Neoplasm Grading
MH  - PTEN Phosphohydrolase/*analysis
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Tissue Array Analysis
OTO - NOTNLM
OT  - PTEN
OT  - Squamous cell carcinoma
OT  - larynx
OT  - microarrays
EDAT- 2017/10/07 06:00
MHDA- 2017/10/17 06:00
CRDT- 2017/10/07 06:00
PHST- 2017/09/03 00:00 [received]
PHST- 2017/09/11 00:00 [revised]
PHST- 2017/09/19 00:00 [accepted]
PHST- 2017/10/07 06:00 [entrez]
PHST- 2017/10/07 06:00 [pubmed]
PHST- 2017/10/17 06:00 [medline]
AID - 37/10/5521 [pii]
AID - 10.21873/anticanres.11983 [doi]
PST - ppublish
SO  - Anticancer Res. 2017 Oct;37(10):5521-5524. doi: 10.21873/anticanres.11983.