PMID- 28985241 OWN - NLM STAT- MEDLINE DCOM- 20190930 LR - 20190930 IS - 1941-7225 (Electronic) IS - 0895-7061 (Print) IS - 0895-7061 (Linking) VI - 31 IP - 2 DP - 2018 Jan 12 TI - Short-Term High-Salt Diet Increases Corin Level to Regulate the Salt-Water Balance in Humans and Rodents. PG - 253-260 LID - 10.1093/ajh/hpx148 [doi] AB - BACKGROUND: Dietary sodium and potassium affect the fluctuation in blood pressure (BP) and renal function. Corin, with its enzymatic activity to convert pro-atrial natriuretic peptide (pro-ANP) to biologically active ANP, regulates BP, cardiac, and renal functions. We investigated whether corin expression responds to a high-salt (HS) diet to regulate salt and water balance. METHODS: Forty-two volunteers followed 3 sequential diets for 7 days each: a low-salt (LS) diet (3.0 g/day NaCl), a HS diet (18.0 g/day NaCl), followed by an HS diet with K+ supplementation (HS + K+) (18.0 g/day NaCl and 4.5 g/day KCl). RESULTS: Corin level was higher with the HS diet than the LS and HS + K+ diets and was positively correlated with systolic BP (SBP) and 24-hour urinary Na+ and microalbumin (U-mALB) excretion. In rodents, serum and renal levels of corin were transiently increased with the HS diet and were decreased if the HS diet was continued for up to 7 days. HS loading increased SBP, 24-hour urinary Na+, U-mALB excretion, and the expression of proprotein convertase subtilisin/kexin-6 (PCSK6), a corin activator. Knockdown of PCSK6 or corin in high salt-treated M1-cortical collecting duct (M1-CCD) cells increased the expression of aquaporin 2 (AQP2) and beta-epithelial Na+ channel (beta-ENaC). CONCLUSIONS: Short-term HS may induce the PCSK6-corin-ANP-AQP2/beta-ENaC pathway in the kidney. Enhanced serum corin level in humans and rodents is positively correlated with HS-induced SBP and 24-hour urinary Na+ and U-mALB excretion, which suggests that corin is involved in the salt-water balance in response to HS intake. CLINICAL TRIALS REGISTRATION: Public Trials Registry Number NCT02915315. CI - (c) American Journal of Hypertension, Ltd 2017. All rights reserved. For Permissions, please email: journals.permissions@oup.com FAU - Zhang, Jiao AU - Zhang J AD - Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. AD - Cardiovascular Research Center, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China. AD - Department of Medicine, University of California, San Diego, La Jolla, California, USA. FAU - Yin, Yanjun AU - Yin Y AD - Cardiovascular Research Center, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China. FAU - Chen, Lili AU - Chen L AD - Cardiovascular Research Center, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China. FAU - Chu, Chao AU - Chu C AD - Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Wang, Yang AU - Wang Y AD - Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Lv, Yongbo AU - Lv Y AD - Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - He, Ming AU - He M AD - Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. AD - Cardiovascular Research Center, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China. AD - Department of Medicine, University of California, San Diego, La Jolla, California, USA. FAU - Martin, Marcy AU - Martin M AD - Department of Medicine, University of California, San Diego, La Jolla, California, USA. FAU - Huang, Po-Hsun AU - Huang PH AD - Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. FAU - Mu, Jian-Jun AU - Mu JJ AD - Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Shyy, John Y-J AU - Shyy JY AD - Cardiovascular Research Center, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China. AD - Department of Medicine, University of California, San Diego, La Jolla, California, USA. FAU - Yuan, Zu-Yi AU - Yuan ZY AD - Department of Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. AD - Cardiovascular Research Center, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China. LA - eng SI - ClinicalTrials.gov/NCT02915315 GR - R01 HL105318/HL/NHLBI NIH HHS/United States GR - R01 HL125643/HL/NHLBI NIH HHS/United States PT - Comparative Study PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PL - United States TA - Am J Hypertens JT - American journal of hypertension JID - 8803676 RN - 0 (Aqp2 protein, rat) RN - 0 (Aquaporin 2) RN - 0 (Epithelial Sodium Channels) RN - 0 (Potassium, Dietary) RN - 0 (Scnn1b protein, rat) RN - 0 (Sodium Chloride, Dietary) RN - EC 3.4.21.- (CORIN protein, human) RN - EC 3.4.21.- (PCSK9 protein, rat) RN - EC 3.4.21.- (Proprotein Convertase 9) RN - EC 3.4.21.- (Serine Endopeptidases) RN - EC 3.4.21.- (corin protein, rat) SB - IM MH - Adaptation, Physiological MH - Adult MH - Albuminuria/enzymology/physiopathology MH - Animals MH - Aquaporin 2/genetics/metabolism MH - Blood Pressure MH - *Diet, Sodium-Restricted MH - Epithelial Sodium Channels/genetics/metabolism MH - Female MH - Humans MH - Kidney/*enzymology/physiopathology MH - Male MH - Middle Aged MH - Natriuresis MH - Potassium, Dietary/*administration & dosage/adverse effects/metabolism MH - Proprotein Convertase 9/genetics/metabolism MH - Rats, Sprague-Dawley MH - Serine Endopeptidases/genetics/*metabolism MH - Sodium Chloride, Dietary/*administration & dosage/adverse effects/metabolism MH - Time Factors MH - Up-Regulation MH - *Water-Electrolyte Balance PMC - PMC5861544 OTO - NOTNLM OT - PCSK6 OT - blood pressure OT - corin OT - high-salt diet OT - hypertension OT - potassium EDAT- 2017/10/07 06:00 MHDA- 2019/10/01 06:00 PMCR- 2018/09/09 CRDT- 2017/10/07 06:00 PHST- 2017/01/17 00:00 [received] PHST- 2017/08/05 00:00 [accepted] PHST- 2017/10/07 06:00 [pubmed] PHST- 2019/10/01 06:00 [medline] PHST- 2017/10/07 06:00 [entrez] PHST- 2018/09/09 00:00 [pmc-release] AID - 4077127 [pii] AID - hpx148 [pii] AID - 10.1093/ajh/hpx148 [doi] PST - ppublish SO - Am J Hypertens. 2018 Jan 12;31(2):253-260. doi: 10.1093/ajh/hpx148.