PMID- 28986217
OWN - NLM
STAT- MEDLINE
DCOM- 20180625
LR  - 20180625
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 71
DP  - 2017 Dec
TI  - A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense 
      against immune challenges.
PG  - 105-115
LID - S1050-4648(17)30595-8 [pii]
LID - 10.1016/j.fsi.2017.09.076 [doi]
AB  - Tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) is a member of 
      the TRAF superfamily that acted as a key signal transduction protein and has been 
      implicated in inflammatory and apoptosis processes in mammals. However, 
      identification of TRAF2s in invertebrates is very limited and its function, in 
      particular that under immune challenges, is still unknown. In this report, a 
      molluscan TRAF2 gene (referred to as AwTRAF2) was cloned and characterized from 
      the freshwater bivalve, Anodonta woodiana. The open reading frame (ORF) of 
      AwTRAF2 was 1683 bp in length, which encoded a putative 560 amino acid-protein. 
      The deduced AwTRAF2 sequence shared similar structural characteristics and close 
      evolutionary relationship with mollusk TRAF2s. The tissue-specific expression 
      analysis revealed that AwTRAF2 mRNA was broadly expressed in all tested tissues, 
      with high expression in gill and hepatopancreas. In addition, in vivo injection 
      experiments directly showed that AwTRAF2 mRNA levels in hepatopancreas were 
      significantly up-regulated in response to bacterial pathogen (Vibrio 
      alginolyticus and Staphylococcus aureus) and PAMPs (Lipopolysaccharides and 
      Peptidoglycan) challenges. Moreover, fluorescence microscopy observations 
      revealed that AwTRAF2 was mainly located in cytoplasm of HEK293T cells and its 
      overexpression significantly increased the transcriptional activities of the 
      NF-kappaB-Luc reporter gene in HEK293T cells. Taken together, this study provided the 
      experimental evidence of the presence of a functional TRAF2 in freshwater 
      bivalves, which revealed its involvement in host response to immune challenges in 
      A. woodiana.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Qu, Fufa
AU  - Qu F
AD  - Department of Biological and Environmental Engineering, Changsha University, 
      Changsha 410022, China; Key Laboratory of Tropical Marine Bio-resources and 
      Ecology and Guangdong Provincial Key Laboratory of Applied Marine Biology, South 
      China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, 
      China; South China Sea Bio-Resource Exploitation and Utilization Collaborative 
      Innovation Center, Guangzhou 510275, China. Electronic address: qufufa@163.com.
FAU - Xiang, Zhiming
AU  - Xiang Z
AD  - Key Laboratory of Tropical Marine Bio-resources and Ecology and Guangdong 
      Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of 
      Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; South China Sea 
      Bio-Resource Exploitation and Utilization Collaborative Innovation Center, 
      Guangzhou 510275, China.
FAU - Zhou, Yingli
AU  - Zhou Y
AD  - Key Laboratory of Tropical Marine Bio-resources and Ecology and Guangdong 
      Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of 
      Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; South China Sea 
      Bio-Resource Exploitation and Utilization Collaborative Innovation Center, 
      Guangzhou 510275, China.
FAU - Qin, Yanping
AU  - Qin Y
AD  - Key Laboratory of Tropical Marine Bio-resources and Ecology and Guangdong 
      Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of 
      Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; South China Sea 
      Bio-Resource Exploitation and Utilization Collaborative Innovation Center, 
      Guangzhou 510275, China.
LA  - eng
PT  - Journal Article
DEP - 20171003
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Pathogen-Associated Molecular Pattern Molecules)
RN  - 0 (TNF Receptor-Associated Factor 2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anodonta/*genetics/*immunology
MH  - Base Sequence
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*immunology
MH  - Immunity, Innate/*genetics
MH  - Pathogen-Associated Molecular Pattern Molecules/pharmacology
MH  - Phylogeny
MH  - Sequence Alignment
MH  - Staphylococcus aureus/physiology
MH  - TNF Receptor-Associated Factor 2/chemistry/*genetics/*immunology
MH  - Vibrio alginolyticus/physiology
OTO - NOTNLM
OT  - Anodonta woodiana
OT  - Immune challenge
OT  - Molecular characterization
OT  - NF-small ka, CyrillicB
OT  - TNF receptor-associated factor 2
EDAT- 2017/10/08 06:00
MHDA- 2018/06/26 06:00
CRDT- 2017/10/08 06:00
PHST- 2017/07/12 00:00 [received]
PHST- 2017/09/23 00:00 [revised]
PHST- 2017/09/29 00:00 [accepted]
PHST- 2017/10/08 06:00 [pubmed]
PHST- 2018/06/26 06:00 [medline]
PHST- 2017/10/08 06:00 [entrez]
AID - S1050-4648(17)30595-8 [pii]
AID - 10.1016/j.fsi.2017.09.076 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2017 Dec;71:105-115. doi: 10.1016/j.fsi.2017.09.076. Epub 
      2017 Oct 3.