PMID- 28986835
OWN - NLM
STAT- MEDLINE
DCOM- 20180611
LR  - 20180620
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1604
DP  - 2018
TI  - Intracellular Detection of Viral Transcription and Replication Using RNA FISH.
PG  - 201-207
LID - 10.1007/978-1-4939-6981-4_14 [doi]
AB  - Many hemorrhagic fever viruses require BSL-3 or BSL-4 laboratory containment for 
      study. The necessary safety precautions associated with this work often 
      contribute to longer assay times and lengthy decontamination procedures. Here we 
      will discuss recent advances in RNA fluorescence in situ hybridization (FISH) 
      that not only allow entirely new investigations into the replication of these 
      viruses but also demonstrate how this method can be applied to any virus with a 
      known sequence and how it can be rapidly performed to minimize time spent in high 
      containment. We have adapted existing protocols for mRNA detection with 
      appropriate changes for examining viruses in a variety of containment 
      laboratories (Shaffer et al., PLoS One 8:e75120, 2013; Raj et al., Nat Methods 
      5:877-879, 2008).
FAU - Lindquist, Michael E
AU  - Lindquist ME
AD  - Virology Division, United States Army Medical Research Institute of Infectious 
      Diseases, 1425 Porter Street, Fort Detrick, MD, USA. 
      michael.e.lindquist3.ctr@mail.mil.
FAU - Schmaljohn, Connie S
AU  - Schmaljohn CS
AD  - Virology Division, United States Army Medical Research Institute of Infectious 
      Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Hantaan virus/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/genetics
MH  - Virus Replication/genetics/physiology
OTO - NOTNLM
OT  - Hemorrhagic fever virus replication
OT  - RNA FISH
OT  - RNA localization
OT  - TurboFISH
OT  - Viral RNA detection
EDAT- 2017/10/08 06:00
MHDA- 2018/06/12 06:00
CRDT- 2017/10/08 06:00
PHST- 2017/10/08 06:00 [entrez]
PHST- 2017/10/08 06:00 [pubmed]
PHST- 2018/06/12 06:00 [medline]
AID - 10.1007/978-1-4939-6981-4_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1604:201-207. doi: 10.1007/978-1-4939-6981-4_14.