PMID- 28987541
OWN - NLM
STAT- MEDLINE
DCOM- 20171212
LR  - 20231213
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 361
IP  - 1
DP  - 2017 Dec 1
TI  - Robo signaling regulates the production of cranial neural crest cells.
PG  - 73-84
LID - S0014-4827(17)30535-9 [pii]
LID - 10.1016/j.yexcr.2017.10.002 [doi]
AB  - Slit/Robo signaling plays an important role in the guidance of developing neurons 
      in developing embryos. However, it remains obscure whether and how Slit/Robo 
      signaling is involved in the production of cranial neural crest cells. In this 
      study, we examined Robo1 deficient mice to reveal developmental defects of mouse 
      cranial frontal and parietal bones, which are derivatives of cranial neural crest 
      cells. Therefore, we determined the production of HNK1(+) cranial neural crest 
      cells in early chick embryo development after knock-down (KD) of Robo1 
      expression. Detection of markers for pre-migratory and migratory neural crest 
      cells, PAX7 and AP-2alpha, showed that production of both was affected by Robo1 KD. 
      In addition, we found that the transcription factor slug is responsible for the 
      aberrant delamination/EMT of cranial neural crest cells induced by Robo1 KD, 
      which also led to elevated expression of E- and N-Cadherin. N-Cadherin expression 
      was enhanced when blocking FGF signaling with dominant-negative FGFR1 in half of 
      the neural tube. Taken together, we show that Slit/Robo signaling influences the 
      delamination/EMT of cranial neural crest cells, which is required for cranial 
      bone development.
CI  - Copyright (c) 2017. Published by Elsevier Inc.
FAU - Li, Yan
AU  - Li Y
AD  - Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of 
      the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, 
      China; The key Laboratory of Assisted Circulation, Ministry of Health, The First 
      Affiliated Hospital of Sun Yat-sen University, Sun Yat-Sen University, Guangzhou 
      510080, China.
FAU - Zhang, Xiao-Tan
AU  - Zhang XT
AD  - Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of 
      the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, 
      China.
FAU - Wang, Xiao-Yu
AU  - Wang XY
AD  - Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of 
      the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, 
      China.
FAU - Wang, Guang
AU  - Wang G
AD  - Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of 
      the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, 
      China.
FAU - Chuai, Manli
AU  - Chuai M
AD  - Division of Cell and Developmental Biology, University of Dundee, Dundee DD1 5EH, 
      UK.
FAU - Munsterberg, Andrea
AU  - Munsterberg A
AD  - School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
FAU - Yang, Xuesong
AU  - Yang X
AD  - Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of 
      the Ministry of Education, Medical College, Jinan University, Guangzhou 510632, 
      China. Electronic address: yang_xuesong@126.com.
LA  - eng
PT  - Journal Article
DEP - 20171005
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Robo2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Craniofacial Abnormalities/genetics/metabolism/*pathology
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Tissue Proteins/*physiology
MH  - Neural Crest/*cytology/metabolism
MH  - Neurogenesis
MH  - Receptors, Immunologic/*physiology
MH  - Roundabout Proteins
OTO - NOTNLM
OT  - Cranial neural crest
OT  - Delamination
OT  - EMT
OT  - Intramembranous ossification
OT  - Slit/Robo
EDAT- 2017/10/11 06:00
MHDA- 2017/12/13 06:00
CRDT- 2017/10/09 06:00
PHST- 2017/06/27 00:00 [received]
PHST- 2017/09/08 00:00 [revised]
PHST- 2017/10/03 00:00 [accepted]
PHST- 2017/10/11 06:00 [pubmed]
PHST- 2017/12/13 06:00 [medline]
PHST- 2017/10/09 06:00 [entrez]
AID - S0014-4827(17)30535-9 [pii]
AID - 10.1016/j.yexcr.2017.10.002 [doi]
PST - ppublish
SO  - Exp Cell Res. 2017 Dec 1;361(1):73-84. doi: 10.1016/j.yexcr.2017.10.002. Epub 
      2017 Oct 5.