PMID- 28989270
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1077-260X (Print)
IS  - 1558-4542 (Electronic)
IS  - 1077-260X (Linking)
VI  - 23
IP  - 2
DP  - 2017 Mar-Apr
TI  - Robust Visualization and Discrimination of Nanoparticles by Interferometric 
      Imaging.
LID - 6900610 [pii]
LID - 10.1109/JSTQE.2016.2639824 [doi]
AB  - Single-molecule and single-nanoparticle biosensors are a growing frontier in 
      diagnostics. Digital biosensors are those which enumerate all specifically 
      immobilized biomolecules or biological nanoparticles, and thereby achieve limits 
      of detection usually beyond the reach of ensemble measurements. Here we review 
      modern optical techniques for single nanoparticle detection and describe the 
      single-particle interferometric reflectance imaging sensor (SP-IRIS). We present 
      challenges associated with reliably detecting faint nanoparticles with SP-IRIS, 
      and describe image acquisition processes and software modifications to address 
      them. Specifically, we describe a image acquisition processing method for the 
      discrimination and accurate counting of nanoparticles that greatly reduces both 
      the number of false positives and false negatives. These engineering improvements 
      are critical steps in the translation of SP-IRIS towards applications in medical 
      diagnostics.
FAU - Trueb, Jacob
AU  - Trueb J
AD  - Department of Mechanical Engineering Boston University, 8 St. Mary's Street, 
      Boston, MA 02215.
FAU - Avci, Oguzhan
AU  - Avci O
AD  - Department of Electrical and Computer Engineering, Boston University, 8 St. 
      Mary's Street, Boston, MA 02215, USA (617-353-5067).
FAU - Sevenler, Derin
AU  - Sevenler D
AD  - Department of Biomedical Engineering at Boston University, 44 Cummington Way, 
      Boston MA 02215.
FAU - Connor, John H
AU  - Connor JH
AD  - Microbiology Department, Boston University School of Medicine, Boston, MA 02118, 
      USA.
FAU - Unlu, M Selim
AU  - Unlu MS
AD  - Department of Electrical and Computer Engineering, Boston University, 8 St. 
      Mary's Street, Boston, MA 02215, USA (617-353-5067).
LA  - eng
GR  - R01 AI096159/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20161214
PL  - United States
TA  - IEEE J Sel Top Quantum Electron
JT  - IEEE journal of selected topics in quantum electronics : a publication of the 
      IEEE Lasers and Electro-optics Society
JID - 101212349
PMC - PMC5627618
MID - NIHMS859012
OTO - NOTNLM
OT  - Optical biosensing
OT  - digital detection
OT  - interferometry
OT  - nanoparticle imaging
OT  - single particle detection
EDAT- 2017/10/11 06:00
MHDA- 2017/10/11 06:01
PMCR- 2018/03/01
CRDT- 2017/10/10 06:00
PHST- 2017/10/10 06:00 [entrez]
PHST- 2017/10/11 06:00 [pubmed]
PHST- 2017/10/11 06:01 [medline]
PHST- 2018/03/01 00:00 [pmc-release]
AID - 6900610 [pii]
AID - 10.1109/JSTQE.2016.2639824 [doi]
PST - ppublish
SO  - IEEE J Sel Top Quantum Electron. 2017 Mar-Apr;23(2):6900610. doi: 
      10.1109/JSTQE.2016.2639824. Epub 2016 Dec 14.