PMID- 28990098
OWN - NLM
STAT- MEDLINE
DCOM- 20180626
LR  - 20180626
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 16
IP  - 6
DP  - 2017 Dec
TI  - Anti‑inflammatory effect of Amomum xanthioides in a mouse atopic dermatitis 
      model.
PG  - 8964-8972
LID - 10.3892/mmr.2017.7695 [doi]
AB  - Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disorder. The 
      present study investigated the effects of Amomum xanthioides extract (AXE) on 
      AD‑like skin inflammation using a Dermatophagoides farinae extract (DFE) and 
      2,4‑dinitrochlorobenzene (DNCB)‑induced mouse AD model. Hematoxylin and eosin 
      staining results demonstrated that repeated DFE/DNCB exposure markedly increased 
      the thickening of the dermis and epidermis, in addition to the infiltration of 
      eosinophils and mast cells. However, oral administration of AXE reduced these 
      histopathological alterations in a dose‑dependent manner. Elevated serum 
      histamine, total and DFE‑specific immunoglobulin E (IgE), and IgG2a were also 
      decreased by treatment with AXE. In addition, reverse transcription‑quantitative 
      polymerase chain reaction (RT‑qPCR) results demonstrated that the mRNA expression 
      of tumor necrosis factor (TNF)‑alpha, interferon (IFN)‑gamma, interleukin (IL)‑4, IL‑13, 
      IL‑31 and IL‑17A was reduced in ear skin following AXE administration in AD mice. 
      Fluorescence‑activated cell sorting demonstrated that the population of 
      CD4+/IL‑4+, CD4+/IFN‑gamma+ and CD4+/IL‑17A+ cells in draining lymph nodes was also 
      significantly decreased in AXE‑treated mice compared with AD mice without AXE 
      treatment. Furthermore, keratinocytes that were stimulated with TNF‑alpha and IFN‑gamma 
      exhibited increased gene expression of pro‑inflammatory cytokines and chemokines, 
      including TNF‑alpha, IL‑1beta, IL‑6, IL‑8, C‑C motif chemokine ligand (CCL)17 and CCL22, 
      as determined by RT‑qPCR. However, upregulation of these genes was reduced by AXE 
      pretreatment. Based on these results, we hypothesize that AXE may be useful in 
      the treatment of allergic skin inflammation, particularly AD.
FAU - Choi, Young-Ae
AU  - Choi YA
AD  - Department of Pharmacology, Cell and Matrix Research Institute, School of 
      Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
FAU - Choi, Jin Kyeong
AU  - Choi JK
AD  - Department of Pharmacology, Cell and Matrix Research Institute, School of 
      Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
FAU - Jang, Yong Hyun
AU  - Jang YH
AD  - Department of Dermatology, School of Medicine, Kyungpook National University, 
      Daegu 41944, Republic of Korea.
FAU - Lee, Soyoung
AU  - Lee S
AD  - Immunoregulatory Materials Research Center, Korea Research Institute of 
      Bioscience and Biotechnology, Jeongeup, Jeollabuk‑do 56212, Republic of Korea.
FAU - Lee, Sang-Rae
AU  - Lee SR
AD  - National Primate Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Cheongju, Chungcheongbuk‑do 28116, Republic of Korea.
FAU - Choi, Jung Ho
AU  - Choi JH
AD  - R&D Center Pharmaceutical Laboratory, Korean Drug Co., Ltd., Seoul 06300, 
      Republic of Korea.
FAU - Park, Jee Hun
AU  - Park JH
AD  - R&D Center Pharmaceutical Laboratory, Korean Drug Co., Ltd., Seoul 06300, 
      Republic of Korea.
FAU - Shin, Tae-Yong
AU  - Shin TY
AD  - Department of Pharmacy, College of Pharmacy, Woosuk University, Samrye, 
      Jeollabuk‑do 55338, Republic of Korea.
FAU - Kim, Sang-Hyun
AU  - Kim SH
AD  - Department of Pharmacology, Cell and Matrix Research Institute, School of 
      Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20171003
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Amomum/*chemistry
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Dermatitis, Atopic/drug therapy/*immunology/pathology
MH  - Disease Models, Animal
MH  - Histamine/blood/immunology
MH  - Humans
MH  - Immunoglobulin E/blood/immunology
MH  - Keratinocytes/drug effects/metabolism
MH  - Mice
MH  - Plant Extracts/*pharmacology
MH  - Skin/metabolism/pathology
EDAT- 2017/10/11 06:00
MHDA- 2018/06/27 06:00
CRDT- 2017/10/10 06:00
PHST- 2017/06/26 00:00 [received]
PHST- 2017/08/31 00:00 [accepted]
PHST- 2017/10/11 06:00 [pubmed]
PHST- 2018/06/27 06:00 [medline]
PHST- 2017/10/10 06:00 [entrez]
AID - 10.3892/mmr.2017.7695 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Dec;16(6):8964-8972. doi: 10.3892/mmr.2017.7695. Epub 2017 Oct 
      3.