PMID- 28990107
OWN - NLM
STAT- MEDLINE
DCOM- 20180720
LR  - 20181202
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 16
IP  - 6
DP  - 2017 Dec
TI  - Estradiol‑enhanced osteogenesis of rat bone marrow stromal cells is associated 
      with the JNK pathway.
PG  - 8589-8594
LID - 10.3892/mmr.2017.7699 [doi]
AB  - Bone marrow stromal cells (BMSCs) can differentiate into osteoblasts. The present 
      study investigated the osteogenic effects of estradiol, as well as the role of 
      the c‑Jun N‑terminal kinase (JNK) signaling pathway in promoting 
      estradiol‑enhanced osteogenesis of rat (r)BMSCs. rBMSCs were treated for 7 days 
      with or without estradiol and further treated with or without the JNK‑specific 
      inhibitor SP600125. The role of estrogen during rBMSC osteogenesis was evaluated 
      by alkaline phosphatase activity and mineralized nodule formation using the 
      Gomori method and Alizarin red S staining, respectively. Subsequently, the mRNA 
      expression levels of transforming growth factor-beta1 (TGF‑beta1) and core‑binding 
      factor alpha1 (Cbfalpha1) were evaluated by reverse transcription‑quantitative polymerase 
      chain reaction, and TGF‑beta1, Cbfalpha1 and phosphorylated (p)‑JNK protein expression 
      was detected by western blotting. All groups treated with SP600125 expressed low 
      levels of TGF‑beta1 and Cbfalpha1 mRNA and protein, and low p‑JNK protein expression. 
      Compared with the control cells, rBMSCs cultured with estradiol exhibited a 
      significant upregulation in the expression levels of osteogenic genes and 
      proteins. The present study demonstrated that estradiol enhanced osteogenic 
      differentiation of rBMSCs and that the JNK signaling pathway was involved in this 
      process, providing insights into the molecular mechanisms involved in rBMSC 
      osteogenesis upon estradiol stimulation.
FAU - Song, Nan
AU  - Song N
AD  - Key Laboratory of Ministry of Education for TCM Viscera‑State Theory and 
      Applications, Ministry of Education of China, Shenyang, Liaoning 110847, P.R. 
      China.
FAU - Wang, Zhi-Min
AU  - Wang ZM
AD  - The Graduate School, Liaoning University of Traditional Chinese Medicine, 
      Shenyang, Liaoning 110847, P.R. China.
FAU - He, Li-Juan
AU  - He LJ
AD  - School of Chinese Medical Formulae, College of Basic Medicine, Liaoning 
      University of Traditional Chinese Medicine, Shenyang, Liaoning 110847, P.R. 
      China.
FAU - Xu, Yan
AU  - Xu Y
AD  - College of Pharmacy, Liaoning University of Traditional Chinese Medicine, 
      Shenyang, Liaoning 110847, P.R. China.
FAU - Ren, Yan-Ling
AU  - Ren YL
AD  - School of Chinese Medical Formulae, College of Basic Medicine, Liaoning 
      University of Traditional Chinese Medicine, Shenyang, Liaoning 110847, P.R. 
      China.
LA  - eng
PT  - Journal Article
DEP - 20171003
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Minerals)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Shape/drug effects
MH  - Core Binding Factor Alpha 1 Subunit/genetics/metabolism
MH  - Estradiol/*pharmacology
MH  - Flow Cytometry
MH  - MAP Kinase Signaling System/*drug effects
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/drug effects/*enzymology
MH  - Minerals/metabolism
MH  - Osteogenesis/*drug effects
MH  - Phosphorylation/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta1/genetics/metabolism
PMC - PMC5779911
EDAT- 2017/10/11 06:00
MHDA- 2018/07/22 06:00
PMCR- 2017/10/03
CRDT- 2017/10/10 06:00
PHST- 2015/12/29 00:00 [received]
PHST- 2017/01/05 00:00 [accepted]
PHST- 2017/10/11 06:00 [pubmed]
PHST- 2018/07/22 06:00 [medline]
PHST- 2017/10/10 06:00 [entrez]
PHST- 2017/10/03 00:00 [pmc-release]
AID - mmr-16-06-8589 [pii]
AID - 10.3892/mmr.2017.7699 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Dec;16(6):8589-8594. doi: 10.3892/mmr.2017.7699. Epub 2017 Oct 
      3.