PMID- 28990703
OWN - NLM
STAT- MEDLINE
DCOM- 20180913
LR  - 20211204
IS  - 1440-1819 (Electronic)
IS  - 1323-1316 (Linking)
VI  - 72
IP  - 4
DP  - 2018 Apr
TI  - Effects of maternal separation and antidepressant drug on epigenetic regulation 
      of the brain-derived neurotrophic factor exon I promoter in the adult rat 
      hippocampus.
PG  - 255-265
LID - 10.1111/pcn.12609 [doi]
AB  - AIM: Early life stress can induce epigenetic changes through genetic and 
      environmental interactions and is a risk factor for depression. Brain-derived 
      neurotrophic factor (BDNF) has been implicated in the pathophysiology of 
      depression and antidepressant drug action. We investigated epigenetic changes at 
      the BDNF exon I promoter in the hippocampus of adult rats subjected to maternal 
      separation (MS) during early life and treated with an antidepressant drug as 
      adults. METHODS: Rat pups were subjected to MS from postnatal day 1 to 21 and 
      received chronic escitalopram (ESC) as adults. We assessed the effects of MS and 
      ESC on BDNF exon I and DNA methyltransferases (DNMT) mRNA levels (quantitative 
      reverse-transcription polymerase chain reaction), acetylated histone H3, and 
      MeCP2 binding to the BDNF promoter I (chromatin immunoprecipitation followed by 
      real-time polymerase chain reaction), and BDNF protein levels (enzyme-linked 
      immunosorbent assay). RESULTS: The levels of BDNF protein, exon I mRNA, histone 
      H3 acetylation, and DNMT1 and DNMT3a mRNA were altered in the MS group compared 
      with the control group. Significant decreases were observed in the BDNF protein, 
      exon I mRNA, and histone H3 acetylation levels and there were significant 
      increases in DNMT1 and DNMT3a mRNA levels. The comparison between the MS + ESC 
      and MS groups revealed significant increases in BDNF protein, exon I mRNA, and 
      histone H3 acetylation levels and significant decreases in MeCP2 and DNMT1 and 
      DNMT3a mRNA levels. CONCLUSION: These findings indicate that MS induced 
      epigenetic changes at the BDNF exon I promoter and these changes were prevented 
      by antidepressant drug treatment during adulthood.
CI  - (c) 2017 The Authors. Psychiatry and Clinical Neurosciences (c) 2017 Japanese Society 
      of Psychiatry and Neurology.
FAU - Park, Sung Woo
AU  - Park SW
AUID- ORCID: 0000-0001-8601-9084
AD  - Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea.
AD  - Departments of Health Science and Technology, Graduate School, Inje University, 
      Busan, Republic of Korea.
AD  - Department of Convergence Biomedical Science, College of Medicine, Inje 
      University, Busan, Republic of Korea.
FAU - Seo, Mi Kyoung
AU  - Seo MK
AUID- ORCID: 0000-0002-3368-4950
AD  - Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea.
FAU - Lee, Jung Goo
AU  - Lee JG
AUID- ORCID: 0000-0003-3393-2667
AD  - Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea.
AD  - Departments of Health Science and Technology, Graduate School, Inje University, 
      Busan, Republic of Korea.
AD  - Department of Psychiatry, College of Medicine, Haeundae Paik Hospital, Inje 
      University, Busan, Republic of Korea.
FAU - Hien, Le Thi
AU  - Hien LT
AUID- ORCID: 0000-0002-7638-4798
AD  - Departments of Health Science and Technology, Graduate School, Inje University, 
      Busan, Republic of Korea.
FAU - Kim, Young Hoon
AU  - Kim YH
AUID- ORCID: 0000-0002-2003-7877
AD  - Department of Psychiatry, Gongju National Hospital, Gongju, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20171114
PL  - Australia
TA  - Psychiatry Clin Neurosci
JT  - Psychiatry and clinical neurosciences
JID - 9513551
RN  - 0 (Antidepressive Agents, Second-Generation)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Histones)
RN  - 0 (Mecp2 protein, rat)
RN  - 0 (Methyl-CpG-Binding Protein 2)
RN  - 0 (RNA, Messenger)
RN  - 0DHU5B8D6V (Citalopram)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
RN  - EC 2.1.1.37 (DNA Methyltransferase 3A)
RN  - EC 2.1.1.37 (Dnmt1 protein, rat)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Antidepressive Agents, Second-Generation/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/drug effects/*metabolism
MH  - Citalopram/*pharmacology
MH  - DNA (Cytosine-5-)-Methyltransferase 1/drug effects/*metabolism
MH  - DNA (Cytosine-5-)-Methyltransferases/drug effects/*metabolism
MH  - DNA Methyltransferase 3A
MH  - Epigenesis, Genetic/drug effects/*physiology
MH  - Exons
MH  - Female
MH  - Hippocampus/drug effects/*metabolism
MH  - Histones/drug effects/*metabolism
MH  - Male
MH  - *Maternal Deprivation
MH  - Methyl-CpG-Binding Protein 2/drug effects/*metabolism
MH  - Pregnancy
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - BDNF exon I
OT  - antidepressant drug
OT  - epigenetic regulation
OT  - hippocampus
OT  - maternal separation
EDAT- 2017/10/11 06:00
MHDA- 2018/09/14 06:00
CRDT- 2017/10/10 06:00
PHST- 2017/07/14 00:00 [received]
PHST- 2017/09/25 00:00 [revised]
PHST- 2017/10/03 00:00 [accepted]
PHST- 2017/10/11 06:00 [pubmed]
PHST- 2018/09/14 06:00 [medline]
PHST- 2017/10/10 06:00 [entrez]
AID - 10.1111/pcn.12609 [doi]
PST - ppublish
SO  - Psychiatry Clin Neurosci. 2018 Apr;72(4):255-265. doi: 10.1111/pcn.12609. Epub 
      2017 Nov 14.