PMID- 2899498
OWN - NLM
STAT- MEDLINE
DCOM- 19880829
LR  - 20181130
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 123
IP  - 2
DP  - 1988 Aug
TI  - Inhibitory effect of hypothalamic medial preoptic area somatostatin on growth 
      hormone-releasing factor in the rat.
PG  - 1103-9
AB  - Possible inhibitory effects of somatostatin (SRIF) on GRF were studied by 
      assessing spontaneous GH secretion and GRF content and release in adult male rats 
      depleted of hypothalamic SRIF by anterolateral hypothalamic deafferentation (AHD) 
      or electrolytic lesions in the medial preoptic area (MPO). Plasma GH levels were 
      measured 7 days postoperatively every 20 min in conscious animals with indwelling 
      iv cannulae. Median eminence SRIF was markedly reduced 8 days postoperatively in 
      both AHD and MPO rats, as determined by immunohistochemistry and RIA (P less than 
      0.01). Although GRF immunoreactivity in the median eminence of AHD and MPO 
      animals appeared well preserved immunocytochemically, hypothalamic GRF content by 
      RIA was significantly decreased at 8 days (P less than 0.01). Spontaneous GH 
      secretion was pulsatile in sham-operated animals. In contrast, basal GH levels in 
      AHD and MPO animals were markedly elevated (P less than 0.01), and secretory 
      pulses were absent. Intravenous injection of specific anti-GRF serum into MPO 
      animals decreased the elevated plasma GH levels (P less than 0.01), indicating 
      increased hypothalamic GRF secretion. GRF release from hypothalamic median 
      eminence-arcuate nucleus complexes in vitro was significantly greater in AHD and 
      MPO animals than in control animals 4 and 8 days postoperatively in response to 
      30 mM K+ (P less than 0.01), but not under basal conditions. These results 
      suggest that hypothalamic medial preoptic area somatostatinergic neurons play a 
      tonic inhibitory role in the regulation of GRF release and that GH hypersecretion 
      observed after MPO and AHD is attributable to changes in both SRIF and GRF.
FAU - Katakami, H
AU  - Katakami H
AD  - Department of Medicine, University of Cincinnati College of Medicine, Ohio 45267.
FAU - Downs, T R
AU  - Downs TR
FAU - Frohman, L A
AU  - Frohman LA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 51110-01-1 (Somatostatin)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9034-39-3 (Growth Hormone-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/metabolism
MH  - Growth Hormone/metabolism
MH  - Growth Hormone-Releasing Hormone/immunology/*physiology
MH  - Hypothalamus, Anterior/*physiology
MH  - Immunization, Passive
MH  - Immunohistochemistry
MH  - Male
MH  - Median Eminence/metabolism
MH  - Preoptic Area/*physiology
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Somatostatin/*physiology
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
AID - 10.1210/endo-123-2-1103 [doi]
PST - ppublish
SO  - Endocrinology. 1988 Aug;123(2):1103-9. doi: 10.1210/endo-123-2-1103.