PMID- 29019224
OWN - NLM
STAT- MEDLINE
DCOM- 20171211
LR  - 20220311
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 22
IP  - 8
DP  - 2016 Aug
TI  - [Diagnostic value of immunohistochemistry and FISH for chromosome 12p in type Ⅱ 
      testicular germ cell tumors].
PG  - 692-697
AB  - OBJECTIVE: To study the pathological morphology, immunohistochemical 
      characteristics, and molecular changes of type Ⅱ testicular germ cell tumors 
      (TGCT) and investigate the possible value of immunohistochemistry and 
      fluorescence in situ hybridization (FISH) in the diagnosis of TGCT. METHODS: We 
      collected for this study 97 cases of TGCT, including 75 cases of seminoma, 17 
      cases of embryonal carcinoma, 11 cases of yolk sac tumor, 16 cases of mature 
      teratoma, 3 cases of immature teratoma, and 1 case of epidermoid cyst, in which 
      normal testicular tissue was found in 20 and non-TGCT in 6. We detected the 
      expressions of different antibodies in various subtypes of TGCT by 
      immunohistochemistry and determined the rate of chromosome 12p abnormality using 
      FISH. RESULTS: The immunophenotypes varied with different subtypes of TGCT. SALL4 
      and PLAP exhibited high sensitivity in all histological subtypes. CD117 and OCT4 
      showed strongly positive expressions in invasive seminoma and germ cell neoplasia 
      in situ (GCNIS) but not in normal seminiferous tubules. GPC3 was significantly 
      expressed in the yolk sac tumor, superior to GATA3 and AFP in both range and 
      intensity. CKpan, OCT4, and CD30 were extensively expressed in embryonal 
      carcinoma, while HCG expressed in choriocarcinoma. The positivity rate of 
      isochromosome 12p and 12p amplification in TGCT was 96.7% (29/30). CONCLUSIONS: 
      The majority of TGCT can be diagnosed by histological observation, but 
      immunohistochemical staining is crucial for more accurate subtypes and valuable 
      for selection of individualized treatment options and evaluation of prognosis. 
      Chromosome 12p abnormality is a specific molecular alteration in type Ⅱ TGCT, 
      which is useful for ruling out other lesions.
FAU - Shen, Qin
AU  - Shen Q
AD  - Department of Pathology, Jinling Hospital, Nanjing University School of Medicine 
      / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, 
      China.
FAU - Rao, Qiu
AU  - Rao Q
AD  - Department of Pathology, Jinling Hospital, Nanjing University School of Medicine 
      / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, 
      China.
FAU - Yu, Bo
AU  - Yu B
AD  - Department of Pathology, Jinling Hospital, Nanjing University School of Medicine 
      / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, 
      China.
FAU - Xia, Qiu-Yuan
AU  - Xia QY
AD  - Department of Pathology, Jinling Hospital, Nanjing University School of Medicine 
      / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, 
      China.
FAU - Bao, Wei
AU  - Bao W
AD  - Department of Pathology, Jinling Hospital, Nanjing University School of Medicine 
      / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, 
      China.
FAU - Lu, Zhen-Feng
AU  - Lu ZF
AD  - Department of Pathology, Jinling Hospital, Nanjing University School of Medicine 
      / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, 
      China.
FAU - Shi, Qun-Li
AU  - Shi QL
AD  - Department of Pathology, Jinling Hospital, Nanjing University School of Medicine 
      / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, 
      China.
FAU - Zhou, Xiao-Jun
AU  - Zhou XJ
AD  - Department of Pathology, Jinling Hospital, Nanjing University School of Medicine 
      / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Genetic Markers)
RN  - Testicular Germ Cell Tumor
SB  - IM
MH  - Biomarkers, Tumor/metabolism
MH  - Carcinoma, Embryonal/diagnosis/genetics/metabolism/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 12
MH  - Endodermal Sinus Tumor/diagnosis/genetics/metabolism/pathology
MH  - Genetic Markers
MH  - Humans
MH  - *Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplasms, Germ Cell and Embryonal/*diagnosis/genetics/metabolism/pathology
MH  - Prognosis
MH  - Seminiferous Tubules/metabolism
MH  - Seminoma/diagnosis/genetics/metabolism/pathology
MH  - Teratoma/diagnosis/genetics/metabolism/pathology
MH  - Testicular Neoplasms/*diagnosis/genetics/metabolism/pathology
OTO - NOTNLM
OT  - fluorescence in situ hybridization
OT  - immunohistochemistry
OT  - isochromosome of chromosome 12p
OT  - testicular germ cell tumor, type Ⅱ
OT  - testis
EDAT- 2016/08/01 00:00
MHDA- 2017/12/12 06:00
CRDT- 2017/10/12 06:00
PHST- 2017/10/12 06:00 [entrez]
PHST- 2016/08/01 00:00 [pubmed]
PHST- 2017/12/12 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2016 Aug;22(8):692-697.