PMID- 29022703
OWN - NLM
STAT- MEDLINE
DCOM- 20190110
LR  - 20190110
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 9
IP  - 43
DP  - 2017 Nov 1
TI  - Probing the Eumelanin-Silica Interface in Chemically Engineered Bulk Hybrid 
      Nanoparticles for Targeted Subcellular Antioxidant Protection.
PG  - 37615-37622
LID - 10.1021/acsami.7b11839 [doi]
AB  - We disclose herein the first example of stable monodispersed hybrid nanoparticles 
      (termed MelaSil-NPs) made up of eumelanin biopolymer intimately integrated into a 
      silica nanoscaffold matrix and endowed with high antioxidant and cytoprotective 
      effects associated with a specific subcellular localization. MelaSil-NPs have 
      been fabricated by an optimized sol-gel methodology involving ammonia-induced 
      oxidative polymerization of a covalent conjugate of the eumelanin building block 
      5,6-dihydroxyindole-2-carboxylic acid (DHICA) with 3-aminopropyltriethoxysilanes 
      (APTS). They displayed a round-shaped (ca. 50-80 nm) morphology, exhibited the 
      typical electron paramagnetic resonance signal of eumelanin biopolymers, and 
      proved effective in promoting decomposition of hydrogen peroxide under 
      physiologically relevant conditions. When administered to human ovarian cancer 
      cells (A2780) or cervical cancer cells (HeLa), MelaSil-NPs were rapidly 
      internalized and colocalized with lysosomes and exerted efficient protecting 
      effects against hydrogen peroxide-induced oxidative stress and cytotoxicity.
FAU - Silvestri, Brigida
AU  - Silvestri B
AD  - Department of Chemical, Materials and Production Engineering, University of 
      Naples "Federico II" , p.le V. Tecchio 80, 80125 Naples, Italy.
FAU - Vitiello, Giuseppe
AU  - Vitiello G
AD  - Department of Chemical, Materials and Production Engineering, University of 
      Naples "Federico II" , p.le V. Tecchio 80, 80125 Naples, Italy.
AD  - CSGI, Consorzio interuniversitario per lo sviluppo dei Sistemi a Grande 
      Interfase, Sesto Fiorentino , via della Lastruccia 3, 50019 Firenze, Italy.
FAU - Luciani, Giuseppina
AU  - Luciani G
AUID- ORCID: 0000-0002-1169-0137
AD  - Department of Chemical, Materials and Production Engineering, University of 
      Naples "Federico II" , p.le V. Tecchio 80, 80125 Naples, Italy.
FAU - Calcagno, Vincenzo
AU  - Calcagno V
AD  - Institute of Biophysics and Medical Physic, University of Leipzig , Hartelstrasse 
      16-18, D-04107 Leipzig, Germany.
FAU - Costantini, Aniello
AU  - Costantini A
AD  - Department of Chemical, Materials and Production Engineering, University of 
      Naples "Federico II" , p.le V. Tecchio 80, 80125 Naples, Italy.
FAU - Gallo, Maria
AU  - Gallo M
AD  - Department of Molecular Medicine and Medical Biotechnology, University of Naples 
      "'Federico II"', Naples , Via Pansini, 5, 80131 Naples, Italy.
FAU - Parisi, Silvia
AU  - Parisi S
AD  - Department of Molecular Medicine and Medical Biotechnology, University of Naples 
      "'Federico II"', Naples , Via Pansini, 5, 80131 Naples, Italy.
AD  - Ceinge Biotecnologie Avanzate , Via Gaetano Salvatore 486, 80145 Naples, Italy.
FAU - Paladino, Simona
AU  - Paladino S
AD  - Department of Molecular Medicine and Medical Biotechnology, University of Naples 
      "'Federico II"', Naples , Via Pansini, 5, 80131 Naples, Italy.
AD  - Ceinge Biotecnologie Avanzate , Via Gaetano Salvatore 486, 80145 Naples, Italy.
FAU - Iacomino, Mariagrazia
AU  - Iacomino M
AD  - Department of Chemical Sciences, University of Naples "Federico II" , Via Cintia 
      4, I-80126 Naples, Italy.
FAU - D'Errico, Gerardino
AU  - D'Errico G
AD  - CSGI, Consorzio interuniversitario per lo sviluppo dei Sistemi a Grande 
      Interfase, Sesto Fiorentino , via della Lastruccia 3, 50019 Firenze, Italy.
AD  - Department of Chemical Sciences, University of Naples "Federico II" , Via Cintia 
      4, I-80126 Naples, Italy.
FAU - Caso, M Federica
AU  - Caso MF
AD  - Nanofaber Spin-off at Italian National Agency for New Technologies, Energy and 
      Sustainable Economic Development (ENEA), Casaccia Research Centre , Via 
      Anguillarese 301, 00123 Rome, Italy.
FAU - Pezzella, Alessandro
AU  - Pezzella A
AUID- ORCID: 0000-0001-6925-922X
AD  - Department of Chemical Sciences, University of Naples "Federico II" , Via Cintia 
      4, I-80126 Naples, Italy.
AD  - National Interuniversity Consortium of Materials Science and Technology (INSTM) , 
      Florence 50121, Italy.
AD  - Institute for Polymers, Composites and Biomaterials (IPCB), CNR , Via Campi 
      Flegrei 34, I-80078 Pozzuoli (NA), Italy.
FAU - d'Ischia, Marco
AU  - d'Ischia M
AUID- ORCID: 0000-0002-7184-0029
AD  - Department of Chemical Sciences, University of Naples "Federico II" , Via Cintia 
      4, I-80126 Naples, Italy.
LA  - eng
PT  - Journal Article
DEP - 20171023
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Indoles)
RN  - 0 (Melanins)
RN  - 12627-86-0 (eumelanin)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Cell Line, Tumor
MH  - Humans
MH  - Indoles
MH  - Melanins
MH  - *Nanoparticles
MH  - Silicon Dioxide
OTO - NOTNLM
OT  - antioxidant
OT  - cytoprotection
OT  - eumelanins
OT  - hybrid nanomaterial
OT  - lysosomal localization
OT  - oxidative stress
OT  - silica nanoparticles
EDAT- 2017/10/13 06:00
MHDA- 2019/01/11 06:00
CRDT- 2017/10/13 06:00
PHST- 2017/10/13 06:00 [pubmed]
PHST- 2019/01/11 06:00 [medline]
PHST- 2017/10/13 06:00 [entrez]
AID - 10.1021/acsami.7b11839 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2017 Nov 1;9(43):37615-37622. doi: 
      10.1021/acsami.7b11839. Epub 2017 Oct 23.