PMID- 29023371 OWN - NLM STAT- MEDLINE DCOM- 20180523 LR - 20181113 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 9 IP - 10 DP - 2017 Oct 12 TI - Expression Profiles of Ligands for Activating Natural Killer Cell Receptors on HIV Infected and Uninfected CD4(+) T Cells. LID - 10.3390/v9100295 [doi] LID - 295 AB - Natural Killer (NK) cell responses to HIV-infected CD4 T cells (iCD4) depend on the integration of signals received through inhibitory (iNKR) and activating NK receptors (aNKR). iCD4 activate NK cells to inhibit HIV replication. HIV infection-dependent changes in the human leukocyte antigen (HLA) ligands for iNKR on iCD4 are well documented. By contrast, less is known regarding the HIV infection related changes in ligands for aNKR on iCD4. We examined the aNKR ligand profiles HIV p24(+) HIV iCD4s that maintained cell surface CD4 (iCD4(+)), did not maintain CD4 (iCD4(-)) and uninfected CD4 (unCD4) T cells for expression of unique long (UL)-16 binding proteins-1 (ULBP-1), ULBP-2/5/6, ULBP-3, major histocompatibility complex (MHC) class 1-related (MIC)-A, MIC-B, CD48, CD80, CD86, CD112, CD155, Intercellular adhesion molecule (ICAM)-1, ICAM-2, HLA-E, HLA-F, HLA-A2, HLA-C, and the ligands to NKp30, NKp44, NKp46, and killer immunoglobulin-like receptor 3DS1 (KIR3DS1) by flow cytometry on CD4 T cells from 17 HIV-1 seronegative donors activated and infected with HIV. iCD4(+) cells had higher expression of aNKR ligands than did unCD4. However, the expression of aNKR ligands on iCD4 where CD4 was downregulated (iCD4(-)) was similar to (ULBP-1, ULBP-2/5/6, ULBP-3, MIC-A, CD48, CD80, CD86 and CD155) or significantly lower than (MIC-B, CD112 and ICAM-2) what was observed on unCD4. Thus, HIV infection can be associated with increased expression of aNKR ligands or either baseline or lower than baseline levels of aNKR ligands, concomitantly with the HIV-mediated downregulation of cell surface CD4 on infected cells. FAU - Tremblay-McLean, Alexandra AU - Tremblay-McLean A AD - Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada. alexandra.tremblay-mclean@mail.mcgill.ca. AD - Division of Experimental Medicine, McGill University, Montreal, QC H4A 3J1, Canada. alexandra.tremblay-mclean@mail.mcgill.ca. FAU - Bruneau, Julie AU - Bruneau J AD - Department de Medecine Familiale et Medecine D'urgence, Universite de Montreal, Montreal, QC H2X 0A9, Canada. julie.bruneau.umontreal@gmail.com. AD - Centre de Recherche de Centre Hospitalier de l'Universite de Montreal, Montreal, QC H2X 0A9, Canada. julie.bruneau.umontreal@gmail.com. FAU - Lebouche, Bertrand AU - Lebouche B AD - Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada. bertrand.lebouche@mcgill.ca. AD - Department of Family Medicine, McGill University, Montreal, QC H4A 3J1, Canada. bertrand.lebouche@mcgill.ca. AD - Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC H4A 3J1, Canada. bertrand.lebouche@mcgill.ca. FAU - Lisovsky, Irene AU - Lisovsky I AD - Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada. lisovsky18@gmail.com. AD - Division of Experimental Medicine, McGill University, Montreal, QC H4A 3J1, Canada. lisovsky18@gmail.com. FAU - Song, Rujun AU - Song R AD - Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada. rujun.song@mail.mcgill.ca. AD - Division of Experimental Medicine, McGill University, Montreal, QC H4A 3J1, Canada. rujun.song@mail.mcgill.ca. FAU - Bernard, Nicole F AU - Bernard NF AD - Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada. nicole.bernard@mcgill.ca. AD - Division of Experimental Medicine, McGill University, Montreal, QC H4A 3J1, Canada. nicole.bernard@mcgill.ca. AD - Chronic Viral Illness Service, McGill University Health Centre, Montreal, QC H4A 3J1, Canada. nicole.bernard@mcgill.ca. AD - Division of Clinical Immunology, McGill University Health Centre, Montreal, QC H3G 1A4, Canada. nicole.bernard@mcgill.ca. LA - eng GR - MOP-142494/CIHR/Canada PT - Journal Article DEP - 20171012 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (HLA Antigens) RN - 0 (HLA-F antigens) RN - 0 (Histocompatibility Antigens Class I) RN - 0 (Ligands) RN - 0 (MHC class I-related chain A) RN - 0 (NK Cell Lectin-Like Receptor Subfamily K) RN - 0 (Receptors, Natural Killer Cell) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) SB - IM MH - CD4-Positive T-Lymphocytes/*immunology/*virology MH - HIV-1/*immunology/physiology MH - HLA Antigens/genetics/immunology MH - Histocompatibility Antigens Class I/genetics/immunology MH - Humans MH - Intercellular Adhesion Molecule-1/genetics MH - Killer Cells, Natural/*immunology MH - Ligands MH - NK Cell Lectin-Like Receptor Subfamily K/genetics/immunology MH - Receptors, Natural Killer Cell/genetics/immunology/*metabolism PMC - PMC5691646 OTO - NOTNLM OT - HIV infection OT - HIV-infected CD4+ T cells OT - activating NK cell receptor ligands OT - natural killer cells COIS- The authors declare no conflict of interests. The funding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results. EDAT- 2017/10/13 06:00 MHDA- 2018/05/24 06:00 PMCR- 2017/10/01 CRDT- 2017/10/13 06:00 PHST- 2017/09/18 00:00 [received] PHST- 2017/09/30 00:00 [revised] PHST- 2017/10/02 00:00 [accepted] PHST- 2017/10/13 06:00 [entrez] PHST- 2017/10/13 06:00 [pubmed] PHST- 2018/05/24 06:00 [medline] PHST- 2017/10/01 00:00 [pmc-release] AID - v9100295 [pii] AID - viruses-09-00295 [pii] AID - 10.3390/v9100295 [doi] PST - epublish SO - Viruses. 2017 Oct 12;9(10):295. doi: 10.3390/v9100295.