PMID- 29024547
OWN - NLM
STAT- MEDLINE
DCOM- 20180727
LR  - 20180727
IS  - 1932-8494 (Electronic)
IS  - 1932-8486 (Linking)
VI  - 301
IP  - 1
DP  - 2018 Jan
TI  - Equine Endometrial Explants Undergo Significant Degenerative Changes in Culture.
PG  - 148-153
LID - 10.1002/ar.23701 [doi]
AB  - This study evaluated equine endometrial explants following 12, 24, and 48 hr in 
      culture. Measurement of an indicator of cell death in explant supernatant, light 
      microscopy, and gene expression of biomarkers of endometrial function and 
      cellular stress were used to compare the effect of six different media on explant 
      viability and morphology. Viability of explants was assessed indirectly through 
      measuring LDH activity in the culture supernatant. Regardless of culture medium 
      composition, a significant increase in LDH activity was observed within 12 hr of 
      culture, indicating occurrence of cell damage. Morphological analysis through 
      light microscopy revealed degenerative changes occurring within 12 hr and, after 
      48 hr, there is nearly complete loss of luminal and superficial glandular 
      epithelium and diffuse detachment of deep glandular epithelium. Transcript 
      abundance of prostaglandin-endoperoxide synthase 2 (PTGS2), estrogen receptor 1 
      (ESR1), and vascular endothelial growth factor (VEGF) was assessed as biomarkers 
      of endometrial function. A marked increase in PTGS2 and VEGF expression occurred; 
      ESR1 displayed more or less steady expression levels. Above-described changes 
      were seen irrespective of cell culture medium used. The marked increase in 
      expression in PTGS2 expression presents a limitation to using endometrial 
      explants in the current culture system to study aspects of endometrial function 
      such as the inflammatory response to insemination. Anat Rec, 2017. (c) 2017 Wiley 
      Periodicals, Inc. Anat Rec, 301:148-153, 2018. (c) 2017 Wiley Periodicals, Inc.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Schwinghamer, Rose
AU  - Schwinghamer R
AD  - Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, 
      Calgary, Alberta T2N 4Z6, Canada.
FAU - Massolo, Alessandro
AU  - Massolo A
AD  - Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, 
      Calgary, Alberta T2N 4Z6, Canada.
FAU - Knight, Cameron
AU  - Knight C
AD  - Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, 
      Calgary, Alberta T2N 4Z6, Canada.
FAU - Klein, Claudia
AU  - Klein C
AUID- ORCID: 0000-0002-8230-8038
AD  - Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, 
      Calgary, Alberta T2N 4Z6, Canada.
LA  - eng
PT  - Journal Article
DEP - 20171102
PL  - United States
TA  - Anat Rec (Hoboken)
JT  - Anatomical record (Hoboken, N.J. : 2007)
JID - 101292775
RN  - 0 (Biomarkers)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Biomarkers/analysis
MH  - Cyclooxygenase 2/metabolism
MH  - Endometrium/cytology/*physiology
MH  - Estrogen Receptor alpha/metabolism
MH  - Female
MH  - Gene Expression/physiology
MH  - Horses/*physiology
MH  - L-Lactate Dehydrogenase/*metabolism
MH  - Microscopy
MH  - RNA, Messenger/metabolism
MH  - Time Factors
MH  - Tissue Culture Techniques/methods/*veterinary
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - degeneration
OT  - endometrium
OT  - explant culture
OT  - mare
EDAT- 2017/10/13 06:00
MHDA- 2018/07/28 06:00
CRDT- 2017/10/13 06:00
PHST- 2017/07/07 00:00 [received]
PHST- 2017/08/02 00:00 [revised]
PHST- 2017/08/09 00:00 [accepted]
PHST- 2017/10/13 06:00 [pubmed]
PHST- 2018/07/28 06:00 [medline]
PHST- 2017/10/13 06:00 [entrez]
AID - 10.1002/ar.23701 [doi]
PST - ppublish
SO  - Anat Rec (Hoboken). 2018 Jan;301(1):148-153. doi: 10.1002/ar.23701. Epub 2017 Nov 
      2.