PMID- 29024606
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20230328
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 96
IP  - 3
DP  - 2018 Jun
TI  - Role of the TSLP-DC-OX40L pathway in asthma pathogenesis and airway inflammation 
      in mice.
PG  - 306-316
LID - 10.1139/bcb-2017-0126 [doi]
AB  - This study aimed to explore the effect of the TSLP-DC-OX40L pathway in asthma 
      pathogenesis and airway inflammation in mice. For this, 65 male BALF/c mice were 
      distributed among the control, asthma, immunoglobulin G (IgG) + asthma (IgG, 500 
      mug/500 muL, intratracheal injection of 50 muL each time), LY294002 (OX40L 
      inhibitor) + asthma (intratracheal injection of 2 mg/kg LY294002), and anti-TSLP 
      + asthma (intratracheal injection of 500 mug/500 muL TSLP antibody, 50 muL each 
      time) groups. ELISA was applied to measure the serum levels of immunoglobulin E 
      (IgE), ovalbumin (OVA)-sIgE, interleukin-4 (IL-4), IL-5, IL-13, and interferon-gamma 
      (IFN-gamma); flow cytometry was employed to detect Treg cells and dendritic cell (DC) 
      and lymphopoiesis. RT-qPCR and Western blot assays were used to measure the 
      levels of TSLP, OX40L, T-bet, GATA-3, NF-kappaB, p38, and ERK. Treatment with 
      LY294002 and anti-TSLP resulted in increases in the numbers of total cells, 
      eosinophils, neutrophils, and lymphocytes in the bronchoalveolar lavage fluid; 
      total serum levels of IgE, OVA-sIgE, IL-4, IL-5, and IL-13; levels of DC cells; 
      lymphopoiesis; and levels of TSLP, OX40L, GATA-3, NF-kappaB, p38, and ERK, whereas 
      there were decreases in the levels of IFN-gamma and CD4(+)CD25(+)Treg cells; 
      CD4(+)Foxp3(+)Treg cells; and T-bet. The TSLP-DC-OX40L pathway may contribute to 
      asthma pathogenesis and airway inflammation by modulating the levels of 
      CD4(+)CD25(+)Treg cells and inflammatory cytokines.
FAU - Feng, Shuang
AU  - Feng S
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
FAU - Bian, Xu-Hua
AU  - Bian XH
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
FAU - Luo, Ying
AU  - Luo Y
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
FAU - Qin, Guang-Hui
AU  - Qin GH
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
FAU - Shi, Rui-Ming
AU  - Shi RM
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
AD  - Department of Pediatrics, the First Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an 710061, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20171012
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Chromones)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-13)
RN  - 0 (Morpholines)
RN  - 0 (OX40 Ligand)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (Tslpr protein, mouse)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
RIN - Biochem Cell Biol. 2023 Aug 1;101(4):377. PMID: 36975124
MH  - Animals
MH  - Asthma/*drug therapy/immunology
MH  - Chromones/*pharmacology
MH  - Cytokines/blood
MH  - Dendritic Cells/drug effects/metabolism
MH  - Disease Models, Animal
MH  - Eosinophils/drug effects
MH  - Immunoglobulin E/blood
MH  - Immunoglobulins/*drug effects
MH  - Inflammation/*drug therapy/metabolism
MH  - Interferon-gamma/blood
MH  - Interleukin-13/blood
MH  - Male
MH  - Mice
MH  - Morpholines/*pharmacology
MH  - OX40 Ligand/drug effects/metabolism
MH  - Ovalbumin/*pharmacology
MH  - Receptors, Cytokine/*drug effects
OTO - NOTNLM
OT  - TSLP-DC-OX40L pathway
OT  - airway inflammation
OT  - asthma
OT  - asthme
OT  - inflammation de voies aeriennes
OT  - modele de souris
OT  - mouse model
OT  - pathogenesis
OT  - pathogenese
OT  - sentier TSLP-CD-OX40L
EDAT- 2017/10/13 06:00
MHDA- 2018/12/12 06:00
CRDT- 2017/10/13 06:00
PHST- 2017/10/13 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2017/10/13 06:00 [entrez]
AID - 10.1139/bcb-2017-0126 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2018 Jun;96(3):306-316. doi: 10.1139/bcb-2017-0126. Epub 2017 
      Oct 12.