PMID- 29028630
OWN - NLM
STAT- MEDLINE
DCOM- 20180815
LR  - 20180815
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 85
DP  - 2018 Jan
TI  - EZH2 regulates dental pulp inflammation by direct effect on inflammatory factors.
PG  - 16-22
LID - S0003-9969(17)30323-0 [pii]
LID - 10.1016/j.archoralbio.2017.10.004 [doi]
AB  - OBJECTIVE: Pulpitis is a multi-factorial disease that could be caused by complex 
      interactions between genetics, epigenetics and environmental factors. We aimed to 
      evaluate the role of Enhancer of Zeste Homolog 2 (EZH2) in the inflammatory 
      response of human dental pulp cells (HDPCs) and dental pulp tissues. METHODS: The 
      expressions of inflammatory cytokines in HDPCs treated by EZH2 complex or EZH2 
      siRNA with or without rhTNF-alpha were examined by quantitative real-time polymerase 
      chain reaction (q-PCR). The levels of secreted inflammatory cytokines including 
      IL-6, IL-8, IL-15, CCL2 and CXCL12 in culture supernatants were measured by 
      Luminex assay. In rat pulpitis model, the effects of EZH2 on dental pulp tissues 
      were verified by histology. We invested the mechanisms of the effect of EZH2 on 
      the inflammatory factors by ChIP assay. RESULTS: EZH2 down-regulation inhibited 
      the expression of inflammatory factors, including IL-6, IL-8, IL-15, CCL2 and 
      CXCL12 in HDPCs. EZH2 complex promoted the expression and secretion of these 
      inflammatory factors in HDPCs, while EZH2 silencing could attenuate the promotion 
      of inflammatory factors that were induced by rhTNF-alpha. In pulpitis models of rats, 
      EZH2 down-regulation inhibited the inflammatory process of dental pulp while EZH2 
      complex showed no significant facilitation of pulpal inflammation. In addition, 
      EZH2 could bind on the promoters of IL-6, IL-8 and CCL2, but not IL-15 and 
      CXCL12, to affect the transcription of these proinflammatory cytokines. 
      CONCLUSIONS: In HDPCs, EZH2 could induce inflammation, while EZH2 down-regulation 
      could attenuate the inflammatory responses. EZH2 plays an important role in this 
      inflammatory process of dental pulp.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Hui, Tianqian
AU  - Hui T
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China; Department of Pediatric Dentistry, Peking University 
      School and Hospital of Stomatology, Peking University, Beijing, China.
FAU - A, Peng
AU  - A P
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China.
FAU - Zhao, Yuan
AU  - Zhao Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China.
FAU - Yang, Jing
AU  - Yang J
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China.
FAU - Ye, Ling
AU  - Ye L
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China.
FAU - Wang, Chenglin
AU  - Wang C
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 
      Sichuan 610041, China. Electronic address: wxonet@163.com.
LA  - eng
PT  - Journal Article
DEP - 20171007
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Cytokines)
RN  - EC 2.1.1.43 (EZH2 protein, human)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chromatin Immunoprecipitation
MH  - Cytokines/*metabolism
MH  - Dental Pulp/*cytology
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Enhancer of Zeste Homolog 2 Protein/*pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Pulpitis/*drug therapy/*metabolism
MH  - Rats
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - Chemokines
OT  - Dental pulp inflammation
OT  - EZH2
OT  - Inflammatory factors
OT  - Interleukins
EDAT- 2017/10/14 06:00
MHDA- 2018/08/16 06:00
CRDT- 2017/10/14 06:00
PHST- 2017/04/11 00:00 [received]
PHST- 2017/09/22 00:00 [revised]
PHST- 2017/10/07 00:00 [accepted]
PHST- 2017/10/14 06:00 [pubmed]
PHST- 2018/08/16 06:00 [medline]
PHST- 2017/10/14 06:00 [entrez]
AID - S0003-9969(17)30323-0 [pii]
AID - 10.1016/j.archoralbio.2017.10.004 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2018 Jan;85:16-22. doi: 10.1016/j.archoralbio.2017.10.004. Epub 
      2017 Oct 7.