PMID- 29031856
OWN - NLM
STAT- MEDLINE
DCOM- 20181005
LR  - 20231213
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 167
DP  - 2018 Feb
TI  - Relationship between Sigma-1 receptor and BDNF in the visual system.
PG  - 25-30
LID - S0014-4835(17)30483-9 [pii]
LID - 10.1016/j.exer.2017.10.012 [doi]
AB  - Glaucoma is an incurable optic neuropathy characterized by dysfunction and death 
      of retinal ganglion cells (RGCs). Brain derived neurotrophic factor (BDNF) is an 
      essential neurotrophin that supports RGC function and survival. Despite BDNF's 
      importance, our knowledge of molecular mechanisms that modulate BDNF processing 
      and secretion is incomplete. Sigma-1 receptor (S1R) is associated with increased 
      BDNF in hippocampus and with BDNF secretion by brain-derived astrocytes and 
      neuronal cell lines. Much less is known about the relationship between S1R and 
      BDNF in the visual system. Here, we examine how S1R activation and deletion alter 
      expression of mature BDNF (mBDNF) and proBDNF in retina and cultured optic nerve 
      head (ONH) astrocytes. For S1R activation, the S1R agonist (+)-pentazocine (PTZ, 
      0.5 mg/kg) was administered by intraperitoneal injection to C57BL/6J mice, 3 
      times per week, for 5 weeks. Expression of proBDNF and mBDNF was also examined in 
      S1R knockout and age-matched C57BL/6J mice. In vitro, cultured ONH astrocytes 
      were treated with 3 muM PTZ for 24 h followed by collection of media and ONH 
      astrocyte lysates. Results showed that treatment with (+)-PTZ increased mBDNF 
      protein in both retina and hippocampus. In contrast, S1R deletion was associated 
      with retinal mBDNF deficits. In ONH astrocytes S1R agonist (+)-PTZ significantly 
      increased levels of secreted BDNF and proBDNF in cell lysates. These findings 
      support a role for S1R in the modulation of BDNF levels within the retina and 
      optic nerve head. Treatment with S1R agonists might provide benefit in diseases 
      such as glaucoma by increasing BDNF levels from endogenous sources.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Mysona, Barbara A
AU  - Mysona BA
AD  - James and Jean Culver Vision Discovery Institute, Augusta, GA, 30912, United 
      States; Department of Ophthalmology, Medical College of Georgia, Augusta 
      University, Augusta, GA, 30912, United States; Department of Cellular Biology and 
      Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, 30912, 
      United States.
FAU - Zhao, Jing
AU  - Zhao J
AD  - James and Jean Culver Vision Discovery Institute, Augusta, GA, 30912, United 
      States; Department of Ophthalmology, Medical College of Georgia, Augusta 
      University, Augusta, GA, 30912, United States; Department of Cellular Biology and 
      Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, 30912, 
      United States.
FAU - Smith, Sylvia
AU  - Smith S
AD  - James and Jean Culver Vision Discovery Institute, Augusta, GA, 30912, United 
      States; Department of Ophthalmology, Medical College of Georgia, Augusta 
      University, Augusta, GA, 30912, United States; Department of Cellular Biology and 
      Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, 30912, 
      United States.
FAU - Bollinger, Kathryn E
AU  - Bollinger KE
AD  - James and Jean Culver Vision Discovery Institute, Augusta, GA, 30912, United 
      States; Department of Ophthalmology, Medical College of Georgia, Augusta 
      University, Augusta, GA, 30912, United States; Department of Cellular Biology and 
      Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, 30912, 
      United States. Electronic address: kbollinger@gru.edu.
LA  - eng
GR  - K08 EY021758/EY/NEI NIH HHS/United States
GR  - R01 EY027406/EY/NEI NIH HHS/United States
GR  - R01 EY028103/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20171012
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptors, sigma)
RN  - RP4A60D26L (Pentazocine)
SB  - IM
MH  - Analgesics, Opioid/pharmacology
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glaucoma/metabolism
MH  - Injections, Intraperitoneal
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Optic Disk/cytology
MH  - Pentazocine/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, sigma/agonists/*physiology
MH  - Retina/*metabolism
MH  - Sigma-1 Receptor
PMC - PMC5757370
MID - NIHMS920758
OTO - NOTNLM
OT  - BDNF
OT  - Glaucoma
OT  - Optic nerve head astrocytes
OT  - Retina
EDAT- 2017/10/17 06:00
MHDA- 2018/10/06 06:00
PMCR- 2019/02/01
CRDT- 2017/10/17 06:00
PHST- 2017/07/10 00:00 [received]
PHST- 2017/10/10 00:00 [revised]
PHST- 2017/10/11 00:00 [accepted]
PHST- 2017/10/17 06:00 [pubmed]
PHST- 2018/10/06 06:00 [medline]
PHST- 2017/10/17 06:00 [entrez]
PHST- 2019/02/01 00:00 [pmc-release]
AID - S0014-4835(17)30483-9 [pii]
AID - 10.1016/j.exer.2017.10.012 [doi]
PST - ppublish
SO  - Exp Eye Res. 2018 Feb;167:25-30. doi: 10.1016/j.exer.2017.10.012. Epub 2017 Oct 
      12.