PMID- 29032552
OWN - NLM
STAT- MEDLINE
DCOM- 20180604
LR  - 20180829
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1690
DP  - 2018
TI  - Investigating Human Dendritic Cell Immune Responses to Borrelia burgdorferi.
PG  - 291-299
LID - 10.1007/978-1-4939-7383-5_21 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that recognize 
      and phagocytose pathogens, and help to orchestrate adaptive immune responses to 
      combat them. DCs are abundant in the skin where Borrelia burgdorferi first enters 
      the body during a tick bite, and are thus critical in determining the initial 
      stages of the innate and adaptive immune responses against Borrelia. Here, we 
      describe two methods to study the response of DCs to Borrelia; an in vitro 
      approach using monocyte-derived DCs (moDCs) and an ex vivo approach using a human 
      skin model.
FAU - Mason, Lauren M K
AU  - Mason LMK
AD  - Center for Experimental and Molecular Medicine, Academic Medical Center, 
      Amsterdam, The Netherlands.
FAU - Hovius, Joppe W R
AU  - Hovius JWR
AD  - Center for Experimental and Molecular Medicine, Academic Medical Center, 
      Amsterdam, The Netherlands. Lyme@amc.uva.nl.
AD  - Division of Infectious Diseases, Academic Medical Center, Amsterdam, The 
      Netherlands. Lyme@amc.uva.nl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Borrelia burgdorferi/*immunology
MH  - Cell Culture Techniques/methods
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Humans
MH  - Immunity, Cellular
MH  - Lyme Disease/*immunology/microbiology
MH  - Organ Culture Techniques/methods
MH  - *Phagocytosis
MH  - Skin/immunology/microbiology
OTO - NOTNLM
OT  - Borrelia
OT  - DCs
OT  - Lyme borreliosis
OT  - Skin model
OT  - moDCs
EDAT- 2017/10/17 06:00
MHDA- 2018/06/05 06:00
CRDT- 2017/10/17 06:00
PHST- 2017/10/17 06:00 [entrez]
PHST- 2017/10/17 06:00 [pubmed]
PHST- 2018/06/05 06:00 [medline]
AID - 10.1007/978-1-4939-7383-5_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1690:291-299. doi: 10.1007/978-1-4939-7383-5_21.