PMID- 29032661
OWN - NLM
STAT- MEDLINE
DCOM- 20180928
LR  - 20220311
IS  - 2005-1212 (Electronic)
IS  - 1976-2283 (Print)
IS  - 1976-2283 (Linking)
VI  - 12
IP  - 1
DP  - 2018 Jan 15
TI  - A Prospective Study of Eosinophilic Esophagitis and the Expression of Tight 
      Junction Proteins in Patients with Gastroesophageal Reflux Disease Symptoms.
PG  - 30-37
LID - 10.5009/gnl16600 [doi]
AB  - BACKGROUND/AIMS: Eosinophilic esophagitis (EoE) is often erroneously diagnosed as 
      gastroesophageal reflux disease (GERD). The aim of this study is to investigate 
      the prevalence of EoE and the expression of tight junction (TJ) proteins in 
      patients with GERD symptoms. METHODS: One hundred patients with GERD symptoms and 
      10 healthy controls were prospectively studied. Sixty-two patients had symptoms 
      refractory to proton pump inhibitors (PPI). All patients underwent esophageal 
      biopsy. Patients were diagnosed with EoE if the number of eosinophil granulocytes 
      per high-power field was >/=15. Immunohistochemical analysis of TJ proteins 
      (claudin-1, claudin-4, occludin, and zonula occludin-1 [ZO-1]) was performed. 
      RESULTS: EoE was diagnosed in six of 100 patients (6%) with GERD symptoms and in 
      six patients (9.7%) of 62 patients with PPI-refractory GERD. Only one had typical 
      EoE endoscopic findings. The proportion of ZO-1-positive cells was significantly 
      lower in the lower than in the middle esophagus (56.0%+/-14.0% vs 66.0%+/-11.5%, 
      p＜0.05). There were no significant correlations between TJ protein expression and 
      GERD symptoms. CONCLUSIONS: The prevalence of EoE among patients with 
      PPI-refractory GERD is approximately 10%. Regardless of endoscopic findings, 
      esophageal biopsy is crucial in diagnosing EoE. The disruption of ZO-1 expression 
      in the lower esophagus is significantly associated with GERD symptoms.
FAU - Okimoto, Kenichiro
AU  - Okimoto K
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Arai, Makoto
AU  - Arai M
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Ishigami, Hideaki
AU  - Ishigami H
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Saito, Keiko
AU  - Saito K
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Minemura, Shoko
AU  - Minemura S
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Maruoka, Daisuke
AU  - Maruoka D
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Matsumura, Tomoaki
AU  - Matsumura T
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Nakagawa, Tomoo
AU  - Nakagawa T
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Katsuno, Tatsuro
AU  - Katsuno T
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Suzuki, Masaki
AU  - Suzuki M
AD  - Department of Diagnostic Pathology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Nakatani, Yukio
AU  - Nakatani Y
AD  - Department of Diagnostic Pathology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
FAU - Yokosuka, Osamu
AU  - Yokosuka O
AD  - Department of Gastroenterology, Chiba University Graduate School of Medicine, 
      Chiba, Japan.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Gut Liver
JT  - Gut and liver
JID - 101316452
RN  - 0 (Biomarkers)
RN  - 0 (Tight Junction Proteins)
SB  - IM
MH  - Aged
MH  - Biomarkers/metabolism
MH  - Biopsy
MH  - Case-Control Studies
MH  - Diagnosis, Differential
MH  - Eosinophilic Esophagitis/diagnosis/*epidemiology/*metabolism
MH  - Esophagus/metabolism
MH  - Female
MH  - Gastroesophageal Reflux/*complications
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Prevalence
MH  - Prospective Studies
MH  - Tight Junction Proteins/*metabolism
PMC - PMC5753681
OTO - NOTNLM
OT  - Eosinophilic esophagitis
OT  - Gastroesophageal reflux
OT  - Proton pump inhibitor
OT  - Tight junctions
OT  - Zonula occludin-1
COIS- CONFLICTS OF INTEREST No potential conflict of interest relevant to this article 
      was reported.
EDAT- 2017/10/17 06:00
MHDA- 2018/10/03 06:00
PMCR- 2018/01/01
CRDT- 2017/10/17 06:00
PHST- 2016/12/14 00:00 [received]
PHST- 2017/02/19 00:00 [revised]
PHST- 2017/03/27 00:00 [accepted]
PHST- 2017/10/17 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2017/10/17 06:00 [entrez]
PHST- 2018/01/01 00:00 [pmc-release]
AID - gnl16600 [pii]
AID - gnl-12-030 [pii]
AID - 10.5009/gnl16600 [doi]
PST - ppublish
SO  - Gut Liver. 2018 Jan 15;12(1):30-37. doi: 10.5009/gnl16600.