PMID- 2903272
OWN - NLM
STAT- MEDLINE
DCOM- 19881220
LR  - 20191210
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 12
IP  - 4
DP  - 1988 Jul-Aug
TI  - Comparison of three commercial amphetamine immunoassays for detection of 
      methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and 
      methylenedioxyethylamphetamine.
PG  - 229-33
AB  - Three commercial immunoassays for detection of amphetamines in urine, Abuscreen 
      radioimmunoassay (RIA), enzyme-multiplied immunoassay technique (EMIT), and the 
      TDx fluorescence polarization immunoassay (FPIA), have been investigated for 
      detection of methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 
      3,4-methylenedioxymethamphetamine (MDMA), and 3,4-methylenedioxyethylamphetamine 
      (MDE). Blank urine was spiked with 0.1 to 3000 micrograms/mL amphetamine analog 
      and used as sample in the assays. With the RIA and FPIA, MDA displayed a higher 
      cross-reactivity to amphetamine than other analogs, but with EMIT, 
      methamphetamine was relatively similar to amphetamine while MDA, MDMA, and MDE 
      were less reactive. The high specificity RIA and the EMIT confirmation reagents 
      for urine amphetamines produced significant, but relatively minor, reduction in 
      the detectability of these analogs. The variation in cross-reactivity seen 
      between the different assays suggests that RIA, EMIT, and FPIA antibodies have 
      different recognition sites; however, all three immunoassays do detect the 
      illicit amphetamine analogs to varying degrees.
FAU - Ruangyuttikarn, W
AU  - Ruangyuttikarn W
AD  - Department of Pharmacology and Toxicology, University of Utah, College of 
      Pharmacy, Salt Lake City 84112.
FAU - Moody, D E
AU  - Moody DE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 0 (Illicit Drugs)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 44RAL3456C (Methamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - ML1I4KK67B (3,4-methylenedioxyethamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/analogs & derivatives/*urine
MH  - Amphetamines/*urine
MH  - Cross Reactions
MH  - Illicit Drugs/*urine
MH  - Immunoassay
MH  - Immunoenzyme Techniques
MH  - Methamphetamine/*urine
MH  - N-Methyl-3,4-methylenedioxyamphetamine
MH  - Radioimmunoassay
MH  - Reagent Kits, Diagnostic
EDAT- 1988/07/01 00:00
MHDA- 1988/07/01 00:01
CRDT- 1988/07/01 00:00
PHST- 1988/07/01 00:00 [pubmed]
PHST- 1988/07/01 00:01 [medline]
PHST- 1988/07/01 00:00 [entrez]
AID - 10.1093/jat/12.4.229 [doi]
PST - ppublish
SO  - J Anal Toxicol. 1988 Jul-Aug;12(4):229-33. doi: 10.1093/jat/12.4.229.