PMID- 29034530
OWN - NLM
STAT- MEDLINE
DCOM- 20180906
LR  - 20180906
IS  - 1439-0396 (Electronic)
IS  - 0931-2439 (Linking)
VI  - 102
IP  - 2
DP  - 2018 Apr
TI  - Gene expression and morphological changes in the intestinal mucosa associated 
      with increased permeability induced by short-term fasting in chickens.
PG  - e653-e661
LID - 10.1111/jpn.12808 [doi]
AB  - Short-term fasting for 4.5 and 9 hr has been demonstrated to increase intestinal 
      permeability (IP) in chickens. This study aimed to investigate the effects of 0, 
      4.5, 9 and 19.5 hr fasting on intestinal gene expression and villus-crypt 
      architecture of enterocytes in jejunal and ileal samples. On day 38, Ross-308 
      male birds were fasted according to their group and then euthanised. Two separate 
      intestinal sections (each 2 cm long, jejunum and ileum) were collected. One 
      section was utilised for villus height and crypt depth measurements. The second 
      section was snap-frozen in liquid nitrogen for quantitative polymerase chain 
      reaction (qPCR) analysis of tight junction proteins (TJP) including claudin-1, 
      claudin-3, occludin, zonula occludens (ZO-1, ZO-2), junctional adhesion molecules 
      (JAM) and E-cadherin. Additionally genes involved in enterocyte protection 
      including glucagon-like peptide (GLP-2), heat-shock protein (HSP-70), intestinal 
      alkaline phosphatase (IAP), mammalian target of rapamycin (mTOR), toll-like 
      receptors (TLR-4), mucin (MUC-2), cluster differentiation (CD-36) and fatty 
      acid-binding protein (FABP-6) were also analysed. Normally distributed data were 
      analysed using one-way analysis of variance ANOVA. Other data were analysed by 
      non-parametric one-way ANOVA. Villus height and crypt depth were increased 
      (p < .05) only in the ileum after fasting for 4.5 and 9 hr compared with 
      non-fasting group. mRNA expression of claudin-3 was significantly reduced in the 
      ileum of birds fasted for 9 and 19.5 hr, suggesting a role in IP modulation. 
      However, all other TJP genes examined were not statistically different from 
      control. Nevertheless, ileal FABP-6 of all fasted groups was significantly 
      reduced, which could possibly be due to reduced bile acid production during 
      fasting.
CI  - (c) 2017 Blackwell Verlag GmbH.
FAU - Gilani, S
AU  - Gilani S
AUID- ORCID: 0000-0002-2785-6883
AD  - School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA, 
      Australia.
AD  - Poultry CRC, University of New England, Armidale, NSW, Australia.
FAU - Howarth, G S
AU  - Howarth GS
AD  - School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA, 
      Australia.
FAU - Nattrass, G
AU  - Nattrass G
AD  - South Australian Research and Development Institute, University of Adelaide, 
      Roseworthy, SA, Australia.
FAU - Kitessa, S M
AU  - Kitessa SM
AD  - South Australian Research and Development Institute, University of Adelaide, 
      Roseworthy, SA, Australia.
FAU - Barekatain, R
AU  - Barekatain R
AD  - School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA, 
      Australia.
AD  - South Australian Research and Development Institute, University of Adelaide, 
      Roseworthy, SA, Australia.
FAU - Forder, R E A
AU  - Forder REA
AD  - School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA, 
      Australia.
FAU - Tran, C D
AU  - Tran CD
AD  - Health and Biosecurity, Commonwealth Scientific and Industrial Research 
      Organisation, Adelaide, SA, Australia.
AD  - Faculty of Health Sciences, School of Medicine, University of Adelaide, Adelaide, 
      SA, Australia.
FAU - Hughes, R J
AU  - Hughes RJ
AD  - School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA, 
      Australia.
AD  - South Australian Research and Development Institute, University of Adelaide, 
      Roseworthy, SA, Australia.
LA  - eng
PT  - Journal Article
DEP - 20171016
PL  - Germany
TA  - J Anim Physiol Anim Nutr (Berl)
JT  - Journal of animal physiology and animal nutrition
JID - 101126979
SB  - IM
MH  - Animals
MH  - Chickens/*physiology
MH  - *Food Deprivation
MH  - Gene Expression Regulation/*physiology
MH  - Intestinal Mucosa/*physiology
MH  - Male
MH  - Permeability
MH  - Polymerase Chain Reaction/methods/veterinary
MH  - Time Factors
MH  - Transcriptome
OTO - NOTNLM
OT  - broiler
OT  - intestinal permeability
OT  - leaky gut
OT  - reduced fasting
OT  - tight junction protein
EDAT- 2017/10/17 06:00
MHDA- 2018/09/07 06:00
CRDT- 2017/10/17 06:00
PHST- 2017/03/16 00:00 [received]
PHST- 2017/08/02 00:00 [accepted]
PHST- 2017/10/17 06:00 [pubmed]
PHST- 2018/09/07 06:00 [medline]
PHST- 2017/10/17 06:00 [entrez]
AID - 10.1111/jpn.12808 [doi]
PST - ppublish
SO  - J Anim Physiol Anim Nutr (Berl). 2018 Apr;102(2):e653-e661. doi: 
      10.1111/jpn.12808. Epub 2017 Oct 16.