PMID- 29035380
OWN - NLM
STAT- MEDLINE
DCOM- 20181217
LR  - 20230216
IS  - 1530-0307 (Electronic)
IS  - 0023-6837 (Linking)
VI  - 98
IP  - 1
DP  - 2018 Jan
TI  - Mixed cell therapy of bone marrow-derived mesenchymal stem cells and articular 
      cartilage chondrocytes ameliorates osteoarthritis development.
PG  - 106-116
LID - 10.1038/labinvest.2017.117 [doi]
AB  - Of the many cell-based treatments that have been tested in an effort to 
      regenerate osteoarthritic articular cartilage, none have ever produced cartilage 
      that compare with native hyaline cartilage. Studies show that different cell 
      types lead to inconsistent results and for cartilage regeneration to be 
      considered successful, there must be an absence of fibrotic tissue. Here we 
      report of a series of experiments in which bone marrow-derived stem cells (BMSCs) 
      and articular cartilage chondrocytes (ACCs) were mixed in a 1:1 ratio and tested 
      for their ability to enhance cartilage regeneration in three different 
      conditions: (1) in an in vitro differentiation model; (2) in an ex vivo cartilage 
      defect model implanted subcutaneously in mice; and (3) as an intra-articular 
      injection in a meniscectomy-induced OA model in rats. The mixed cells were 
      compared with monocultures of BMSCs and ACCs. In all three experimental models 
      there was significantly enhanced cartilage regeneration and decreased fibrosis in 
      the mixed BMSCs+ACCs group compared with the monocultures. Molecular analysis 
      showed a reduction in vascularization and hypertrophy, coupled with higher 
      chondrogenic gene expression resulting from the BMSCs+ACCs treatment. Together, 
      our data suggest that mixed BMSCs+ACCs treatment is highly chondro-protective and 
      is more effective in regenerating damaged cartilage in both the ex vivo cartilage 
      defect and post-trauma OA disease models. The results from this approach could 
      potentially be used for regeneration of cartilage in OA patients.
FAU - Prasadam, Indira
AU  - Prasadam I
AD  - Institute of Health and Biomedical Innovation, Queensland University of 
      Technology, Brisbane, QLD, Australia.
FAU - Akuien, Akoy
AU  - Akuien A
AD  - Institute of Health and Biomedical Innovation, Queensland University of 
      Technology, Brisbane, QLD, Australia.
FAU - Friis, Thor E
AU  - Friis TE
AD  - Institute of Health and Biomedical Innovation, Queensland University of 
      Technology, Brisbane, QLD, Australia.
FAU - Fang, Wei
AU  - Fang W
AD  - Institute of Health and Biomedical Innovation, Queensland University of 
      Technology, Brisbane, QLD, Australia.
AD  - School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei, China.
FAU - Mao, Xinzhan
AU  - Mao X
AD  - Institute of Health and Biomedical Innovation, Queensland University of 
      Technology, Brisbane, QLD, Australia.
AD  - Department of Orthopaedic Surgery, The Second Xiangya Hospital, Changsha, China.
AD  - Department of Rheumatism, The Xiangya Hospital, Central-South University, 
      Changsha, China.
FAU - Crawford, Ross W
AU  - Crawford RW
AD  - Institute of Health and Biomedical Innovation, Queensland University of 
      Technology, Brisbane, QLD, Australia.
AD  - Prince Charles Hospital, Brisbane, QLD, Australia.
FAU - Xiao, Yin
AU  - Xiao Y
AD  - Institute of Health and Biomedical Innovation, Queensland University of 
      Technology, Brisbane, QLD, Australia.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171016
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
SB  - IM
MH  - Aged
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Cartilage, Articular/blood supply/*metabolism/pathology/physiology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chondrocytes/metabolism/pathology/*transplantation
MH  - Coculture Techniques
MH  - *Disease Models, Animal
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Hypertrophy/metabolism/pathology/prevention & control
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Neovascularization, Pathologic/metabolism/pathology/prevention & control
MH  - Osteoarthritis, Knee/metabolism/pathology/physiopathology/*therapy
MH  - Proof of Concept Study
MH  - Rats, Wistar
MH  - Regeneration
MH  - Transplantation, Heterologous
EDAT- 2017/10/17 06:00
MHDA- 2018/12/18 06:00
CRDT- 2017/10/17 06:00
PHST- 2017/05/08 00:00 [received]
PHST- 2017/08/14 00:00 [revised]
PHST- 2017/09/14 00:00 [accepted]
PHST- 2017/10/17 06:00 [pubmed]
PHST- 2018/12/18 06:00 [medline]
PHST- 2017/10/17 06:00 [entrez]
AID - S0023-6837(22)01156-4 [pii]
AID - 10.1038/labinvest.2017.117 [doi]
PST - ppublish
SO  - Lab Invest. 2018 Jan;98(1):106-116. doi: 10.1038/labinvest.2017.117. Epub 2017 
      Oct 16.