PMID- 29037587
OWN - NLM
STAT- MEDLINE
DCOM- 20171219
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1525
DP  - 2017 Nov 24
TI  - Lipidomic analysis of biological samples: Comparison of liquid chromatography, 
      supercritical fluid chromatography and direct infusion mass spectrometry methods.
PG  - 96-108
LID - S0021-9673(17)31506-6 [pii]
LID - 10.1016/j.chroma.2017.10.022 [doi]
AB  - Lipidomic analysis of biological samples in a clinical research represents 
      challenging task for analytical methods given by the large number of samples and 
      their extreme complexity. In this work, we compare direct infusion (DI) and 
      chromatography - mass spectrometry (MS) lipidomic approaches represented by three 
      analytical methods in terms of comprehensiveness, sample throughput, and 
      validation results for the lipidomic analysis of biological samples represented 
      by tumor tissue, surrounding normal tissue, plasma, and erythrocytes of kidney 
      cancer patients. Methods are compared in one laboratory using the identical 
      analytical protocol to ensure comparable conditions. Ultrahigh-performance liquid 
      chromatography/MS (UHPLC/MS) method in hydrophilic interaction liquid 
      chromatography mode and DI-MS method are used for this comparison as the most 
      widely used methods for the lipidomic analysis together with 
      ultrahigh-performance supercritical fluid chromatography/MS (UHPSFC/MS) method 
      showing promising results in metabolomics analyses. The nontargeted analysis of 
      pooled samples is performed using all tested methods and 610 lipid species within 
      23 lipid classes are identified. DI method provides the most comprehensive 
      results due to identification of some polar lipid classes, which are not 
      identified by UHPLC and UHPSFC methods. On the other hand, UHPSFC method provides 
      an excellent sensitivity for less polar lipid classes and the highest sample 
      throughput within 10min method time. The sample consumption of DI method is 125 
      times higher than for other methods, while only 40muL of organic solvent is used 
      for one sample analysis compared to 3.5mL and 4.9mL in case of UHPLC and UHPSFC 
      methods, respectively. Methods are validated for the quantitative lipidomic 
      analysis of plasma samples with one internal standard for each lipid class. 
      Results show applicability of all tested methods for the lipidomic analysis of 
      biological samples depending on the analysis requirements.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Lisa, Miroslav
AU  - Lisa M
AD  - Department of Analytical Chemistry, Faculty of Chemical Technology, University of 
      Pardubice, Studentska 573, 53210 Pardubice, Czech Republic. Electronic address: 
      miroslav.lisa@gmail.com.
FAU - Cifkova, Eva
AU  - Cifkova E
AD  - Department of Analytical Chemistry, Faculty of Chemical Technology, University of 
      Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
FAU - Khalikova, Maria
AU  - Khalikova M
AD  - Department of Analytical Chemistry, Faculty of Chemical Technology, University of 
      Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
FAU - Ovcacikova, Magdalena
AU  - Ovcacikova M
AD  - Department of Analytical Chemistry, Faculty of Chemical Technology, University of 
      Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
FAU - Holcapek, Michal
AU  - Holcapek M
AD  - Department of Analytical Chemistry, Faculty of Chemical Technology, University of 
      Pardubice, Studentska 573, 53210 Pardubice, Czech Republic.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20171008
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Lipids)
SB  - IM
MH  - *Chromatography, Liquid
MH  - *Chromatography, Supercritical Fluid
MH  - Computational Biology/*methods
MH  - Lipids/*analysis
MH  - *Mass Spectrometry
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Direct infusion
OT  - Lipidomic analysis
OT  - Lipidomics
OT  - Mass spectrometry
OT  - UHPLC
OT  - UHPSFC
EDAT- 2017/10/19 06:00
MHDA- 2017/12/20 06:00
CRDT- 2017/10/18 06:00
PHST- 2017/01/20 00:00 [received]
PHST- 2017/09/25 00:00 [revised]
PHST- 2017/10/07 00:00 [accepted]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2017/12/20 06:00 [medline]
PHST- 2017/10/18 06:00 [entrez]
AID - S0021-9673(17)31506-6 [pii]
AID - 10.1016/j.chroma.2017.10.022 [doi]
PST - ppublish
SO  - J Chromatogr A. 2017 Nov 24;1525:96-108. doi: 10.1016/j.chroma.2017.10.022. Epub 
      2017 Oct 8.