PMID- 29038459
OWN - NLM
STAT- MEDLINE
DCOM- 20190701
LR  - 20240327
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Oct 16
TI  - Small-scale variation of ammonia oxidisers within intertidal sediments dominated 
      by ammonia-oxidising bacteria Nitrosomonas sp. amoA genes and transcripts.
PG  - 13200
LID - 10.1038/s41598-017-13583-x [doi]
LID - 13200
AB  - While numerous studies have investigated the abundance of ammonia oxidising 
      bacteria and archaea (AOB/AOA) via the ammonia monooxygenase gene amoA, less is 
      known about their small-scale variation and if amoA gene abundance equates to 
      activity. Here we present a spatial and temporal study of ammonia oxidation in 
      two small intertidal bays, Rusheen and Clew bay, Ireland. Potential Nitrification 
      Rate (PNR) was ten-fold higher in Rusheen bay (Clew: 0.27 +/- SD 0.55; Rusheen: 
      2.46 +/- SD 3.4 NO(2)(-) microg(-1) g(-1) day(-1), P < 0.001) than in Clew bay but amoA 
      gene abundances were similar between bays, and comparable to those in other 
      coastal ecosystems. Within bays AOB genes increased towards the muddy sediments 
      and were positively correlated with PNR and pH. Less spatial variation was 
      observed in AOA abundances which nevertheless positively correlated with pH and 
      temperature and negatively with salinity and ammonia. Transcriptionally active 
      AOB and AOA were quantified from all sites in Rusheen bay, February 2014, 
      following the general trends observed at DNA level. AOB phylotypes predominantly 
      from the known Nitrosomonas group were distributed across the bay, while 
      Nitrosomonas group B phylotypes were absent from low salinity sites. AOA genes 
      and transcripts were primarily affiliated with Thaumarchaeota group I.1a.
FAU - Duff, Aoife M
AU  - Duff AM
AD  - Microbiology, School of Natural Sciences, NUI Galway, Galway, Ireland.
AD  - Infrastructure and Environment, School of Engineering, University of Glasgow, 
      Glasgow, United Kingdom.
FAU - Zhang, Li-Mei
AU  - Zhang LM
AD  - State Key Laboratory of Urban and Regional Ecology, Research Centre for 
      Eco-Environmental Science, Chinese Academy of Sciences, 18 Shuangqing Rd., Haidan 
      Beijing, 100085, P.R. China.
FAU - Smith, Cindy J
AU  - Smith CJ
AUID- ORCID: 0000-0003-4905-0730
AD  - Microbiology, School of Natural Sciences, NUI Galway, Galway, Ireland. 
      Cindy.Smith@glasgow.ac.uk.
AD  - Infrastructure and Environment, School of Engineering, University of Glasgow, 
      Glasgow, United Kingdom. Cindy.Smith@glasgow.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171016
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Protons)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Geologic Sediments/*analysis
MH  - Nitrosomonas/*metabolism
MH  - Oxidation-Reduction
MH  - Protons
MH  - Salinity
MH  - Sequence Analysis, DNA
PMC - PMC5643298
COIS- The authors declare that they have no competing interests.
EDAT- 2017/10/19 06:00
MHDA- 2019/07/02 06:00
PMCR- 2017/10/16
CRDT- 2017/10/18 06:00
PHST- 2017/07/05 00:00 [received]
PHST- 2017/09/25 00:00 [accepted]
PHST- 2017/10/18 06:00 [entrez]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2019/07/02 06:00 [medline]
PHST- 2017/10/16 00:00 [pmc-release]
AID - 10.1038/s41598-017-13583-x [pii]
AID - 13583 [pii]
AID - 10.1038/s41598-017-13583-x [doi]
PST - epublish
SO  - Sci Rep. 2017 Oct 16;7(1):13200. doi: 10.1038/s41598-017-13583-x.