PMID- 29039495
OWN - NLM
STAT- MEDLINE
DCOM- 20180720
LR  - 20180720
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 40
IP  - 6
DP  - 2017 Dec
TI  - Protective effects of neem (Azadirachta indica A. Juss.) leaf extract against 
      cigarette smoke- and lipopolysaccharide-induced pulmonary inflammation.
PG  - 1932-1940
LID - 10.3892/ijmm.2017.3178 [doi]
AB  - Neem (Azadirachta indica A. Juss.) leaf has been reported to exert 
      anti-inflammatory, antibacterial and antioxidant effects. The purpose of this 
      study was to investigate the protective effects of neem leaf extract (NLE) 
      against cigarette smoke (CS)- and lipopolysaccharide (LPS)-induced pulmonary 
      inflammation. Treatment with NLE significantly attenuated the infiltration of 
      inflammatory cells, such as neutrophils and macrophages in bronchoalveolar lavage 
      fluid (BALF). NLE also reduced the production of reactive oxygen species and the 
      activity of neutrophil elastase in BALF. Moreover, NLE attenuated the release of 
      pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin (IL)-6 in BALF. NLE inhibited the recruitment of inflammatory cells 
      and the expression of monocyte chemoattractant protein-1 (MCP-1) in the lungs of 
      mice with CS- and LPS-induced pulmonary inflammation. NLE also decreased the 
      expression of inducible nitric oxide synthase (iNOS) in the lungs of the mice CS- 
      and LPS-induced pulmonary inflammation. Furthermore, treatment with NLE 
      significantly attenuated the activation of extracellular signal-regulated 
      kinase (ERK) and c-Jun N-terminal kinase (JNK) in the lungs mice exposed to CS 
      and LPS. NLE also inhibited the phosphorylation of nuclear factor (NF)-kappaB and 
      inhibitor of NF-kappaB (IkappaB) in the lungs of mice expose to CS and LPS. These 
      findings thus suggest that NLE has potential for use in the treatment of chronic 
      obstructive pulmonary disease.
FAU - Lee, Jae-Won
AU  - Lee JW
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
FAU - Ryu, Hyung Won
AU  - Ryu HW
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
FAU - Park, So-Yeon
AU  - Park SY
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
FAU - Park, Hyun Ah
AU  - Park HA
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
FAU - Kwon, Ok-Kyoung
AU  - Kwon OK
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
FAU - Yuk, Heung Joo
AU  - Yuk HJ
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
FAU - Shrestha, Krishna K
AU  - Shrestha KK
AD  - Ethnobotanical Society of Nepal (ESON), Central Department of Botany, Tribhuvan 
      University, Kathmandu 44618, Nepal.
FAU - Park, Minwoo
AU  - Park M
AD  - SciTech Korea, Gangbuk-gu, Seoul 142-705, Republic of Korea.
FAU - Kim, Jung Hee
AU  - Kim JH
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
FAU - Lee, Sangwoo
AU  - Lee S
AD  - International Biological Material Research Center, Korea Research Institute of 
      Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
FAU - Oh, Sei-Ryang
AU  - Oh SR
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
FAU - Ahn, Kyung-Seop
AU  - Ahn KS
AD  - Natural Medicine Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungju‑si, Chungbuk 363‑883, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20171010
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Azadirachta/*chemistry
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Interleukin-6/genetics
MH  - Lipopolysaccharides/toxicity
MH  - Lung/drug effects/pathology
MH  - Mice
MH  - Plant Extracts/*administration & dosage/chemistry
MH  - Plant Leaves/chemistry
MH  - Pneumonia/chemically induced/*drug therapy/genetics/pathology
MH  - Pulmonary Disease, Chronic Obstructive/*drug therapy/genetics/pathology
MH  - Reactive Oxygen Species/metabolism
MH  - Smoking/adverse effects
MH  - Tumor Necrosis Factor-alpha/genetics
EDAT- 2017/10/19 06:00
MHDA- 2018/07/22 06:00
CRDT- 2017/10/18 06:00
PHST- 2016/03/30 00:00 [received]
PHST- 2017/09/25 00:00 [accepted]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2018/07/22 06:00 [medline]
PHST- 2017/10/18 06:00 [entrez]
AID - 10.3892/ijmm.2017.3178 [doi]
PST - ppublish
SO  - Int J Mol Med. 2017 Dec;40(6):1932-1940. doi: 10.3892/ijmm.2017.3178. Epub 2017 
      Oct 10.