PMID- 29039609
OWN - NLM
STAT- MEDLINE
DCOM- 20180720
LR  - 20181113
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 38
IP  - 6
DP  - 2017 Dec
TI  - Quercetrin from Toona sinensis leaves induces cell cycle arrest and apoptosis via 
      enhancement of oxidative stress in human colorectal cancer SW620 cells.
PG  - 3319-3326
LID - 10.3892/or.2017.6042 [doi]
AB  - Finding effective strategies against colorectal cancer (CRC) is still an emergent 
      health problem. In the present study, we investigated the anticancer activity of 
      quercetrin from Toona sinensis leaves (QTL) and explored the underlying mechanism 
      in human CRC cell line SW620. The cells were treated with various concentrations 
      of QTL and the cytotoxic effects of QTL were determined using the MTT assay. 
      Apoptosis and cell cycle status were detected by flow cytometry. Reactive oxygen 
      species (ROS) levels and mitochondrial membrane potential (∆Psim) were assessed 
      using DCF-DA and JC-1 fluorescence spectrophotometry, respectively. Western blot 
      analysis was used to quantify the expression of apoptosis‑related proteins. 
      RT-PCR was applied to determine the mRNA levels of glutathione peroxidase (GPx) 
      and catalase (CAT). QTL exhibited growth inhibitory effects and caused cell cycle 
      arrest in the G2/M phase, which was accompanied by increased expression of p53 
      and p21 proteins. QTL promoted apoptosis which was consistent with the 
      upregulated expression of Bax, cytochrome c, caspase-9, Apaf-1 and caspase-3. In 
      addition, QTL induced the loss of mitochondrial membrane potential and triggered 
      ROS generation, as revealed by the downregulated mRNA expression and enzymatic 
      activity of GPx and CAT. Furthermore, both N‑acetyl cysteine (NAC) and GSH 
      attenuated the QTL-induced growth inhibition observed in SW620 cells along with 
      the increase of ROS levels. These findings revealed that QTL inhibited the growth 
      of CRC cells and facilitated apoptosis by enhancing oxidative stress. QTL may 
      therefore have potential for use in CRC chemotherapy.
FAU - Zhang, Yali
AU  - Zhang Y
AD  - Key Laboratory of Biomedical Information Engineering of Ministry of Education, 
      School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710049, P.R. China.
FAU - Guo, Yucheng
AU  - Guo Y
AD  - Key Laboratory of Biomedical Information Engineering of Ministry of Education, 
      School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710049, P.R. China.
FAU - Wang, Mimi
AU  - Wang M
AD  - Key Laboratory of Biomedical Information Engineering of Ministry of Education, 
      School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710049, P.R. China.
FAU - Dong, Huanhuan
AU  - Dong H
AD  - Key Laboratory of Biomedical Information Engineering of Ministry of Education, 
      School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 
      Shaanxi 710049, P.R. China.
FAU - Zhang, Jingfang
AU  - Zhang J
AD  - College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, P.R. 
      China.
FAU - Zhang, Liyu
AU  - Zhang L
AD  - Shaanxi Institute of Pediatric Diseases, Xi'an Children's Hospital, Xi'an, 
      Shaanxi 710049, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171017
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (APAF1 protein, human)
RN  - 0 (Apoptotic Protease-Activating Factor 1)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 2Y8906LC5P (quercitrin)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Apoptotic Protease-Activating Factor 1/genetics
MH  - Caspase 3/genetics
MH  - Cell Cycle Checkpoints/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Colorectal Neoplasms/*drug therapy/genetics/pathology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Glutathione Peroxidase/genetics
MH  - Humans
MH  - Meliaceae/chemistry
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Oxidative Stress/*drug effects
MH  - Plant Extracts/*administration & dosage/chemistry
MH  - Quercetin/administration & dosage/*analogs & derivatives/chemistry
MH  - Reactive Oxygen Species/metabolism
PMC - PMC5783577
EDAT- 2017/10/19 06:00
MHDA- 2018/07/22 06:00
PMCR- 2017/10/17
CRDT- 2017/10/18 06:00
PHST- 2017/04/01 00:00 [received]
PHST- 2017/09/15 00:00 [accepted]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2018/07/22 06:00 [medline]
PHST- 2017/10/18 06:00 [entrez]
PHST- 2017/10/17 00:00 [pmc-release]
AID - or-38-06-3319 [pii]
AID - 10.3892/or.2017.6042 [doi]
PST - ppublish
SO  - Oncol Rep. 2017 Dec;38(6):3319-3326. doi: 10.3892/or.2017.6042. Epub 2017 Oct 17.