PMID- 29040286
OWN - NLM
STAT- MEDLINE
DCOM- 20171030
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 10
DP  - 2017
TI  - Human babesiosis: Indication of a molecular mimicry between thrombospondin 
      domains from a novel Babesia microti BmP53 protein and host platelets molecules.
PG  - e0185372
LID - 10.1371/journal.pone.0185372 [doi]
LID - e0185372
AB  - Human babesiosis is caused by the apicomplexan parasite Babesia microti, which is 
      of major public health concern in the United States and elsewhere, resulting in 
      malaise and fatigue, followed by a fever and hemolytic anemia. In this paper we 
      focus on the characterization of a novel B. microti thrombospondin domain 
      (TSP1)-containing protein (BmP53) from the new annotation of the B. microti 
      genome (locus 'BmR1_04g09041'). This novel protein (BmP53) had a single TSP1 and 
      a transmembrane domain, with a short cytoplasmic tail containing a sub-terminal 
      glutamine residue, but no signal peptide and Von Willebrand factor type A domains 
      (VWA), which are found in classical thrombospondin-related adhesive proteins 
      (TRAP). Co-localization assays of BmP53 and Babesia microti secreted antigen 1 
      (BmSA1) suggested that BmP53 might be a non-secretory membranous protein. 
      Molecular mimicry between the TSP1 domain from BmP53 and host platelets molecules 
      was indicated through different measures of sequence homology, phylogenetic 
      analysis, 3D structure and shared epitopes. Indeed, hamster isolated platelets 
      cross-reacted with mouse anti-BmP53-TSP1. Molecular mimicry are used to help 
      parasites to escape immune defenses, resulting in immune evasion or autoimmunity. 
      Furthermore, specific host reactivity was also detected against the TSP1-free 
      part of BmP53 in infected hamster sera. In conclusion, the TSP1 domain mimicry 
      might help in studying the mechanisms of parasite-induced thrombocytopenia, with 
      the TSP1-free truncate of the protein representing a potential safe candidate for 
      future vaccine studies.
FAU - Mousa, Ahmed Abdelmoniem
AU  - Mousa AA
AD  - Institut de Biologie Computationnelle (IBC), LIRMM, CNRS, Universite de 
      Montpellier, Montpellier, France.
AD  - National Research Center for Protozoan Diseases, Obihiro University of 
      Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
AD  - Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Roche, Daniel Barry
AU  - Roche DB
AD  - Institut de Biologie Computationnelle (IBC), LIRMM, CNRS, Universite de 
      Montpellier, Montpellier, France.
AD  - Centre de Recherche en Biologie cellulaire de Montpellier, CNRS-UMR 5237, 
      Montpellier, France.
FAU - Terkawi, Mohamad Alaa
AU  - Terkawi MA
AD  - Institut de Biologie Computationnelle (IBC), LIRMM, CNRS, Universite de 
      Montpellier, Montpellier, France.
FAU - Kameyama, Kyohko
AU  - Kameyama K
AD  - National Research Center for Protozoan Diseases, Obihiro University of 
      Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
FAU - Kamyingkird, Ketsarin
AU  - Kamyingkird K
AD  - National Research Center for Protozoan Diseases, Obihiro University of 
      Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
AD  - Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, 
      Bangkok, Thailand.
FAU - Vudriko, Patrick
AU  - Vudriko P
AD  - National Research Center for Protozoan Diseases, Obihiro University of 
      Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
FAU - Salama, Akram
AU  - Salama A
AD  - Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Cao, Shinuo
AU  - Cao S
AD  - National Research Center for Protozoan Diseases, Obihiro University of 
      Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
FAU - Orabi, Sahar
AU  - Orabi S
AD  - Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Khalifa, Hanem
AU  - Khalifa H
AD  - Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Ahmed, Mohamed
AU  - Ahmed M
AD  - Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Attia, Mabrouk
AU  - Attia M
AD  - Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Elkirdasy, Ahmed
AU  - Elkirdasy A
AD  - Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Nishikawa, Yoshifumi
AU  - Nishikawa Y
AD  - Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Xuan, Xuenan
AU  - Xuan X
AD  - Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary 
      Medicine, University of Sadat City, Sadat City, Menoufia, Egypt.
FAU - Cornillot, Emmanuel
AU  - Cornillot E
AUID- ORCID: 0000-0002-1202-1162
AD  - Institut de Biologie Computationnelle (IBC), LIRMM, CNRS, Universite de 
      Montpellier, Montpellier, France.
AD  - Institut de Recherche en Cancerologie de Montpellier (IRCM-INSERM U1194), 
      Institut regional du Cancer Montpellier (ICM) and Universite de Montpellier, 
      Montpellier, France.
LA  - eng
PT  - Journal Article
DEP - 20171017
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Thrombospondin 1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
EIN - PLoS One. 2017 Dec 5;12 (12 ):e0189383. PMID: 29206874
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Protozoan/chemistry/genetics/*immunology
MH  - Babesia microti/genetics/*immunology/isolation & purification
MH  - Babesiosis/immunology/*parasitology
MH  - Binding Sites
MH  - Blood Platelets/*parasitology
MH  - Cloning, Molecular
MH  - Cricetulus
MH  - Erythrocytes/parasitology
MH  - Escherichia coli/genetics/metabolism
MH  - Gene Expression
MH  - Glutathione Transferase/genetics/metabolism
MH  - Humans
MH  - *Immune Evasion
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Mimicry
MH  - Protein Binding
MH  - Protein Interaction Domains and Motifs
MH  - Protein Structure, Secondary
MH  - Protozoan Proteins/chemistry/genetics/*immunology
MH  - Recombinant Fusion Proteins/chemistry/genetics/immunology
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Thrombospondin 1/chemistry/genetics/*immunology
PMC - PMC5644982
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/10/19 06:00
MHDA- 2017/10/31 06:00
PMCR- 2017/10/17
CRDT- 2017/10/18 06:00
PHST- 2017/03/22 00:00 [received]
PHST- 2017/09/12 00:00 [accepted]
PHST- 2017/10/18 06:00 [entrez]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2017/10/31 06:00 [medline]
PHST- 2017/10/17 00:00 [pmc-release]
AID - PONE-D-17-10804 [pii]
AID - 10.1371/journal.pone.0185372 [doi]
PST - epublish
SO  - PLoS One. 2017 Oct 17;12(10):e0185372. doi: 10.1371/journal.pone.0185372. 
      eCollection 2017.