PMID- 29040365
OWN - NLM
STAT- MEDLINE
DCOM- 20180518
LR  - 20211204
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 49
IP  - 12
DP  - 2017 Dec 1
TI  - Role of the Akt/mTOR signaling pathway in human papillomavirus-associated nasal 
      and sinonasal inverted papilloma.
PG  - 1067-1074
LID - 10.1093/abbs/gmx108 [doi]
AB  - Nasal and sinonasal inverted papilloma (NSIP) is a benign tumor in which surface 
      epithelial cells grow downward into the underlying supportive tissue with varying 
      degrees of metaplasia. Human papillomavirus (HPV) has been proposed as the causal 
      agent in the pathogenesis of this disease. Many studies have shown that HPV can 
      activate the Akt/mechanistic target of rapamycin (mTOR) signaling pathway, but 
      the role of this pathway in HPV-associated NSIP is largely unknown. In this 
      study, we enrolled 40 control tissue samples and 80 NSIP tissue samples. HPV 
      genotyping showed that 47 of the 80 examined cases of NSIP were HPV-positive 
      (58.8%), and the most common subtype was HPV11 (20/53, 37.7%). The 
      immunohistochemistry showed statistically significant differences in 
      phosphorylated Akt and phosphorylated S6 ribosomal protein staining among control 
      samples, HPV-positive NSIP and HPV-negative NSIP. The HPV11 L1-L2 plasmid 
      increased the proliferation of normal human nasopharyngeal epithelial NP69-SV40T 
      cells and human nasopharyngeal cancer CNE1 cells. Meanwhile, rapamycin, an mTOR 
      inhibitor, reversed the increased cell proliferation induced by the HPV11 L1-L2 
      plasmid. Western blot analysis showed that Akt/mTOR/S6 were overexpressed in 
      NP69-SV40T cells and CNE1 cells infected with the HPV11 L1-L2 plasmid. These data 
      demonstrate that HPV promotes cell proliferation through the Akt/mTOR signaling 
      pathway in NSIP.
CI  - (c) The Author 2017. Published by Oxford University Press on behalf of the 
      Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological 
      Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, 
      please e-mail: journals.permissions@oup.com.
FAU - Liu, Yongliang
AU  - Liu Y
AD  - Department of Otolaryngolgogy, Qilu Hospital of Shandong University, Jinan, 
      China.
AD  - Department of Otolaryngolgogy, Zibo Central Hospital affiliated to Shandong 
      University, Zibo, China.
FAU - Duan, Lihua
AU  - Duan L
AD  - Department of Otolaryngolgogy, Zibo Central Hospital affiliated to Shandong 
      University, Zibo, China.
FAU - Tian, Jie
AU  - Tian J
AD  - Department of Otolaryngolgogy, Zibo Central Hospital affiliated to Shandong 
      University, Zibo, China.
FAU - Song, Daoliang
AU  - Song D
AD  - Department of Otolaryngolgogy, Zibo Central Hospital affiliated to Shandong 
      University, Zibo, China.
FAU - Zhang, Min
AU  - Zhang M
AD  - Department of Otolaryngolgogy, Zibo Central Hospital affiliated to Shandong 
      University, Zibo, China.
FAU - Zhao, Shenlin
AU  - Zhao S
AD  - Department of Otolaryngolgogy, Zibo Central Hospital affiliated to Shandong 
      University, Zibo, China.
FAU - Yin, Zhaofu
AU  - Yin Z
AD  - Department of Otolaryngolgogy, Zibo Central Hospital affiliated to Shandong 
      University, Zibo, China.
FAU - Xiang, Xinxin
AU  - Xiang X
AD  - Central of Translation Medicine, Zibo Central Hospital affiliated to Shandong 
      University, Zibo, China.
FAU - Li, Xuezhong
AU  - Li X
AD  - Department of Otolaryngolgogy, Qilu Hospital of Shandong University, Jinan, 
      China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Cell Proliferation
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Nose Neoplasms/*etiology/virology
MH  - Papilloma, Inverted/*etiology/virology
MH  - Papillomaviridae/genetics/*isolation & purification
MH  - Proto-Oncogene Proteins c-akt/*physiology
MH  - Retrospective Studies
MH  - Signal Transduction/*physiology
MH  - TOR Serine-Threonine Kinases/*physiology
MH  - Young Adult
OTO - NOTNLM
OT  - Akt
OT  - cell proliferation
OT  - human papillomavirus (HPV)
OT  - mechanistic target of rapamycin (mTOR)
OT  - nasal and sinonasal inverted papilloma (NSIP)
EDAT- 2017/10/19 06:00
MHDA- 2018/05/19 06:00
CRDT- 2017/10/18 06:00
PHST- 2017/05/12 00:00 [received]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2018/05/19 06:00 [medline]
PHST- 2017/10/18 06:00 [entrez]
AID - 4523732 [pii]
AID - 10.1093/abbs/gmx108 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2017 Dec 1;49(12):1067-1074. doi: 
      10.1093/abbs/gmx108.