PMID- 29042658
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Oct 17
TI  - Exosomes secreted by human adipose mesenchymal stem cells promote scarless 
      cutaneous repair by regulating extracellular matrix remodelling.
PG  - 13321
LID - 10.1038/s41598-017-12919-x [doi]
LID - 13321
AB  - Scar formation is an intractable medical problem that appears after skin wounds 
      have healed. Recent research has shown that exosomes secreted by human adipose 
      mesenchymal stem cells (ASC-Exos) can benefit wound healing. To further explore 
      the therapeutic potential of ASC-Exos, we investigated their effects on 
      mitigating scar formation, and the underlying mechanisms of these effects. We 
      found that intravenous injection of ASC-Exos decreased the size of scars and 
      increased the ratio of collagen III to collagen I in murine incisional wounds. 
      Exosome treatment also prevented the differentiation of fibroblasts into 
      myofibroblasts and increased the ratio of transforming growth factor-beta3 (TGF-beta3) 
      to TGF-beta1 in vivo. Additionally, we found that ASC-Exos increased the matrix 
      metalloproteinases-3 (MMP3) expression of skin dermal fibroblasts by activating 
      the ERK/MAPK pathway, leading to a high ratio of MMP3 to tissue inhibitor of 
      matrix metalloproteinases-1 (TIMP1), which is also beneficial for the remodelling 
      of extracellular matrix (ECM). In conclusion, our results demonstrated that 
      ASC-Exos promote ECM reconstruction in cutaneous wound repair by regulating the 
      ratios of collagen type III: type I, TGF-beta3:TGF-beta1 and MMP3:TIMP1, and by 
      regulating fibroblast differentiation to mitigate scar formation. Therefore, the 
      application of ASC-Exos may be a novel therapeutic approach for scarless wound 
      repair.
FAU - Wang, Lu
AU  - Wang L
AD  - Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Hu, Li
AU  - Hu L
AD  - Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Zhou, Xin
AU  - Zhou X
AD  - Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Xiong, Zehuan
AU  - Xiong Z
AD  - Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Zhang, Chenguang
AU  - Zhang C
AD  - Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Shehada, Hassan M A
AU  - Shehada HMA
AD  - Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China.
FAU - Hu, Bo
AU  - Hu B
AD  - College of Stomatology, Chongqing Medical University, Chongqing, 401147, P.R. 
      China.
FAU - Song, Jinlin
AU  - Song J
AD  - College of Stomatology, Chongqing Medical University, Chongqing, 401147, P.R. 
      China. soongjl@163.com.
FAU - Chen, Lili
AU  - Chen L
AD  - Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China. lily-c1030@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171017
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
EIN - Sci Rep. 2018 May 1;8(1):7066. PMID: 29717149
EIN - Sci Rep. 2021 Feb 1;11(1):3245. PMID: 33526797
PMC - PMC5645460
COIS- The authors declare that they have no competing interests.
EDAT- 2017/10/19 06:00
MHDA- 2017/10/19 06:01
PMCR- 2017/10/17
CRDT- 2017/10/19 06:00
PHST- 2017/04/26 00:00 [received]
PHST- 2017/09/11 00:00 [accepted]
PHST- 2017/10/19 06:00 [entrez]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2017/10/19 06:01 [medline]
PHST- 2017/10/17 00:00 [pmc-release]
AID - 10.1038/s41598-017-12919-x [pii]
AID - 12919 [pii]
AID - 10.1038/s41598-017-12919-x [doi]
PST - epublish
SO  - Sci Rep. 2017 Oct 17;7(1):13321. doi: 10.1038/s41598-017-12919-x.