PMID- 29042674
OWN - NLM
STAT- MEDLINE
DCOM- 20190701
LR  - 20190701
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Oct 17
TI  - Knockout of maternal CD163 protects fetuses from infection with porcine 
      reproductive and respiratory syndrome virus (PRRSV).
PG  - 13371
LID - 10.1038/s41598-017-13794-2 [doi]
LID - 13371
AB  - After infection of the porcine dam at about 90 days of gestation, porcine 
      reproductive and respiratory syndrome virus (PRRSV) crosses the placenta and 
      begins to infect fetuses. Outcomes of include abortion, fetal death and 
      respiratory disease in newborn piglets. CD163 is the receptor for the virus. In 
      this study, CD163-positive fetuses, recovered between 109 days of gestation or 20 
      days after maternal infection, were completely protected from PRRSV in dams 
      possessing a complete knockout of the CD163 receptor. The results demonstrate a 
      practical means to eliminate PRRSV-associated reproductive disease, a major 
      source of economic hardship to agriculture.
FAU - Prather, Randall S
AU  - Prather RS
AUID- ORCID: 0000-0002-6012-4035
AD  - Division of Animal Science, College of Food Agriculture and Natural Resources, 
      University of Missouri, Columbia, MO, 65211, USA.
FAU - Wells, Kevin D
AU  - Wells KD
AD  - Division of Animal Science, College of Food Agriculture and Natural Resources, 
      University of Missouri, Columbia, MO, 65211, USA.
FAU - Whitworth, Kristin M
AU  - Whitworth KM
AD  - Division of Animal Science, College of Food Agriculture and Natural Resources, 
      University of Missouri, Columbia, MO, 65211, USA.
FAU - Kerrigan, Maureen A
AU  - Kerrigan MA
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS, 66506, USA.
FAU - Samuel, Melissa S
AU  - Samuel MS
AD  - Division of Animal Science, College of Food Agriculture and Natural Resources, 
      University of Missouri, Columbia, MO, 65211, USA.
FAU - Mileham, Alan
AU  - Mileham A
AD  - Genus, plc, DeForest, Wisconsin, USA.
FAU - Popescu, Luca N
AU  - Popescu LN
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS, 66506, USA.
FAU - Rowland, Raymond R R
AU  - Rowland RRR
AD  - Department of Diagnostic Medicine and Pathobiology, College of Veterinary 
      Medicine, Kansas State University, Manhattan, KS, 66506, USA. 
      browland@vet.k-state.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171017
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Antigens, CD/*genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/*genetics/metabolism
MH  - Gene Knockout Techniques
MH  - Genotype
MH  - Host-Pathogen Interactions/*genetics
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Receptors, Cell Surface/*genetics/metabolism
MH  - Swine
PMC - PMC5645351
COIS- Alan Mileham is employed by Genus plc, the company that provided funding for the 
      project. Randy Prather is an inventor on a patent related to the CD163 knockout 
      pig. The remaining authors, Kevin Wells, Kristin M. Whitworth, Maureen Kerrigan, 
      Melissa Samuel, Luca Popescu, Raymond Rowland declare no potential conflict of 
      interest.
EDAT- 2017/10/19 06:00
MHDA- 2019/07/02 06:00
PMCR- 2017/10/17
CRDT- 2017/10/19 06:00
PHST- 2017/06/01 00:00 [received]
PHST- 2017/10/02 00:00 [accepted]
PHST- 2017/10/19 06:00 [entrez]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2019/07/02 06:00 [medline]
PHST- 2017/10/17 00:00 [pmc-release]
AID - 10.1038/s41598-017-13794-2 [pii]
AID - 13794 [pii]
AID - 10.1038/s41598-017-13794-2 [doi]
PST - epublish
SO  - Sci Rep. 2017 Oct 17;7(1):13371. doi: 10.1038/s41598-017-13794-2.