PMID- 29043215
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2287-1098 (Print)
IS  - 2287-8602 (Electronic)
IS  - 2287-1098 (Linking)
VI  - 22
IP  - 3
DP  - 2017 Sep
TI  - Inhibitory Effect of Hizikia fusiformis Solvent-Partitioned Fractions on Invasion 
      and MMP Activity of HT1080 Human Fibrosarcoma Cells.
PG  - 184-190
LID - 10.3746/pnf.2017.22.3.184 [doi]
AB  - Matrix metalloproteinases (MMPs) are endopeptidases that take significant roles 
      in extracellular matrix degradation and therefore linked to several complications 
      such as metastasis of cancer progression, oxidative stress, and hepatic fibrosis. 
      Hizikia fusiformis, a brown algae, was reported to possess bioactivities, 
      including but not limited to, antiviral, antimicrobial, and anti-inflammatory 
      partly due to bioactive polysaccharide contents. In this study, the potential of 
      H. fusiformis against cancer cell invasion was evaluated through the MMP 
      inhibitory effect in HT1080 fibrosarcoma cells in vitro. H. fusiformis crude 
      extract was fractionated with organic solvents, H(2)O, n-BuOH, 85% aqueous MeOH, 
      and n-hexane (n-Hex). The non-toxicity of the fractions was confirmed by MTT 
      assay. All fractions inhibited the enzymatic activities of MMP-2 and MMP-9 
      according to the gelatin zymography assay. Cell migration was also significantly 
      inhibited by the n-Hex fraction. In addition, both gene and protein expressions 
      of MMP-2 and -9, and tissue inhibitor of MMPs (TIMPs) were evaluated by reverse 
      transcription-polymerase chain reaction and Western blotting, respectively. The 
      fractions suppressed the mRNA and protein levels of MMP-2, MMP-9 while elevating 
      the TIMP-1 and TIMP-2, with the H(2)O fraction being the least effective while 
      n-Hex fraction the most. Collectively, the n-Hex fraction from brown algae H. 
      fusiformis could be a potential inhibitor of MMPs, suggesting the presence of 
      various derivatives of polysaccharides in high amounts.
FAU - Lee, Seul-Gi
AU  - Lee SG
AD  - Department of Food and Nutrition, College of Medical and Life Sciences, Silla 
      University, Busan 46958, Korea.
FAU - Karadeniz, Fatih
AU  - Karadeniz F
AD  - Department of Food and Nutrition, College of Medical and Life Sciences, Silla 
      University, Busan 46958, Korea.
AD  - Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, 
      Busan 46958, Korea.
FAU - Oh, Jung Hwan
AU  - Oh JH
AD  - Department of Food and Nutrition, College of Medical and Life Sciences, Silla 
      University, Busan 46958, Korea.
FAU - Yu, Ga Hyun
AU  - Yu GH
AD  - Department of Food and Nutrition, College of Medical and Life Sciences, Silla 
      University, Busan 46958, Korea.
FAU - Kong, Chang-Suk
AU  - Kong CS
AD  - Department of Food and Nutrition, College of Medical and Life Sciences, Silla 
      University, Busan 46958, Korea.
AD  - Marine Biotechnology Center for Pharmaceuticals and Foods, Silla University, 
      Busan 46958, Korea.
LA  - eng
PT  - Journal Article
DEP - 20170930
PL  - Korea (South)
TA  - Prev Nutr Food Sci
JT  - Preventive nutrition and food science
JID - 101586663
PMC - PMC5642799
OTO - NOTNLM
OT  - Hizikia fusiformis
OT  - MMP
OT  - TIMP
OT  - cell migration
OT  - zymography
COIS- AUTHOR DISCLOSURE STATEMENT The authors declare no conflict of interest.
EDAT- 2017/10/19 06:00
MHDA- 2017/10/19 06:01
PMCR- 2017/09/01
CRDT- 2017/10/19 06:00
PHST- 2017/04/12 00:00 [received]
PHST- 2017/08/21 00:00 [accepted]
PHST- 2017/10/19 06:00 [entrez]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2017/10/19 06:01 [medline]
PHST- 2017/09/01 00:00 [pmc-release]
AID - pnfs-22-184 [pii]
AID - 10.3746/pnf.2017.22.3.184 [doi]
PST - ppublish
SO  - Prev Nutr Food Sci. 2017 Sep;22(3):184-190. doi: 10.3746/pnf.2017.22.3.184. Epub 
      2017 Sep 30.