PMID- 29045937
OWN - NLM
STAT- MEDLINE
DCOM- 20171218
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 43
IP  - 4
DP  - 2017
TI  - Adenovirus-Mediated Expression of BMP-2 and BFGF in Bone Marrow Mesenchymal Stem 
      Cells Combined with Demineralized Bone Matrix For Repair of Femoral Head 
      Osteonecrosis in Beagle Dogs.
PG  - 1648-1662
LID - 10.1159/000484026 [doi]
AB  - BACKGROUND: This study investigated the effect of using adenovirus-mediated 
      expression of bone morphogenetic protein 2 (Ad-BMP-2) and basic fibroblast growth 
      factor (bFGF) in bone marrow mesenchymal stem cells (BMSCs) in combination with a 
      demineralized bone matrix (DBM) to repair osteonecrosis of the femoral head 
      (ONFH) in Beagle dogs. METHODS: A total of 30 Beagle dogs were selected for the 
      isolation of BMSCs, which were cultured and transfected with the recombinant 
      adenovirus vector Ad-BMP2-bFGF-GFP (carrying BMP-2 and bFGF) or a control 
      adenovirus plasmid (encoding green fluorescent protein (Ad-GFP)). The expression 
      of the transfected BMP-2 and bFGF proteins was detected by Western blotting. 
      After transfection, the BMSCs were induced to undergo osteoblastic 
      differentiation. The DBM was prepared to construct a DBM/BMSC complex. Beagle 
      models of canine femoral head defects and necrosis were established and divided 
      into control, DBM, DBM/BMSC, vector Ad-BMP2-bFGF-GFP and Ad-GFP groups. The 
      composite graft was then implanted, and new bone morphology was visualized via 
      X-ray at 3, 6 and 12 weeks after the operation. Hematoxylin and eosin (HE) 
      staining and Masson's trichrome staining were used to identify new bone 
      formation. Immunohistochemistry was performed to calculate the density of new 
      blood vessels. The compressive and bending strength of the BMSCs was evaluated at 
      12 weeks after the operation. RESULTS: BMSCs were successfully isolated. The 
      protein expression of BMP-2 and bFGF was significantly higher in the 
      Ad-BMP-2/bFGF group than the normal and Ad-GFP groups. Compared with the control 
      group, at 12 weeks after the operation, the DBM, DBM/BMSC, vector 
      Ad-BMP2-bFGF-GFP and Ad-GFP groups showed a larger area of new bone, higher X-ray 
      scores, greater neovascularization density, and increased compressive and bending 
      strength. The most significant modifications occurred in thevector 
      Ad-BMP2-bFGF-GFP group. CONCLUSION: The results indicate that the use of 
      Ad-BMP-2/bFGF-modified BMSCs in conjunction with DBM could successfully repair 
      ONFH in a dog model by promoting bone formation and angiogenesis.
CI  - (c) 2017 The Author(s). Published by S. Karger AG, Basel.
FAU - Peng, Wu-Xun
AU  - Peng WX
AD  - Department of Traumatic Orthopedics, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, China.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Statistics, Guizhou Health Information Center, Guiyang, China.
LA  - eng
PT  - Journal Article
DEP - 20171018
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Bone Matrix/chemistry/*transplantation
MH  - Bone Morphogenetic Protein 2/*genetics
MH  - Cells, Cultured
MH  - Dogs
MH  - Femur Head/*pathology
MH  - Fibroblast Growth Factor 2/*genetics
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/metabolism
MH  - Osteogenesis
MH  - Osteonecrosis/pathology/*therapy
MH  - Tissue Scaffolds/chemistry
MH  - *Transfection
OTO - NOTNLM
OT  - Basic fibroblast growth factor
OT  - Bone marrow mesenchymal stem cells
OT  - Bone morphogenetic protein 2
OT  - Demineralized bone matrix
OT  - Osteonecrosis of the femoral head
EDAT- 2017/10/19 06:00
MHDA- 2017/12/19 06:00
CRDT- 2017/10/19 06:00
PHST- 2017/01/04 00:00 [received]
PHST- 2017/07/25 00:00 [accepted]
PHST- 2017/10/19 06:00 [pubmed]
PHST- 2017/12/19 06:00 [medline]
PHST- 2017/10/19 06:00 [entrez]
AID - 000484026 [pii]
AID - 10.1159/000484026 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2017;43(4):1648-1662. doi: 10.1159/000484026. Epub 2017 Oct 
      18.