PMID- 29046388
OWN - NLM
STAT- MEDLINE
DCOM- 20190103
LR  - 20210217
IS  - 1535-9484 (Electronic)
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 17
IP  - 1
DP  - 2018 Jan
TI  - The Early Dendritic Cell Signaling Induced by Virulent Francisella tularensis 
      Strain Occurs in Phases and Involves the Activation of Extracellular 
      Signal-Regulated Kinases (ERKs) and p38 In the Later Stage.
PG  - 81-94
LID - 10.1074/mcp.RA117.000160 [doi]
AB  - Dendritic cells (DCs) infected by Francisella tularensis are poorly activated and 
      do not undergo classical maturation process. Although reasons of such 
      unresponsiveness are not fully understood, their impact on the priming of 
      immunity is well appreciated. Previous attempts to explain the behavior of 
      Francisella-infected DCs were hypothesis-driven and focused on events at later 
      stages of infection. Here, we took an alternative unbiased approach by applying 
      methods of global phosphoproteomics to analyze the dynamics of cell signaling in 
      primary DCs during the first hour of infection by Francisella tularensis 
      Presented results show that the early response of DCs to Francisella occurs in 
      phases and that ERK and p38 signaling modules induced at the later stage are 
      differentially regulated by virulent and attenuated DeltadsbA strain. These findings 
      imply that the temporal orchestration of host proinflammatory pathways represents 
      the integral part of Francisella life-cycle inside hijacked DCs.
CI  - (c) 2018 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Fabrik, Ivo
AU  - Fabrik I
AD  - From the double daggerDepartment of Molecular Pathology and Biology, Faculty of Military 
      Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic.
FAU - Link, Marek
AU  - Link M
AD  - From the double daggerDepartment of Molecular Pathology and Biology, Faculty of Military 
      Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic.
FAU - Putzova, Daniela
AU  - Putzova D
AD  - From the double daggerDepartment of Molecular Pathology and Biology, Faculty of Military 
      Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic.
FAU - Plzakova, Lenka
AU  - Plzakova L
AD  - From the double daggerDepartment of Molecular Pathology and Biology, Faculty of Military 
      Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic.
FAU - Lubovska, Zuzana
AU  - Lubovska Z
AD  -  section signInstitute of Molecular Genetics ASCR v.v.i., Microscopy Centre, Electron 
      Microscopy Core Facility, 142 20 Prague 4, Czech Republic.
FAU - Philimonenko, Vlada
AU  - Philimonenko V
AD  -  section signInstitute of Molecular Genetics ASCR v.v.i., Microscopy Centre, Electron 
      Microscopy Core Facility, 142 20 Prague 4, Czech Republic.
AD  -  paragraph signInstitute of Molecular Genetics ASCR v.v.i., Department of Biology of the Cell 
      Nucleus, 142 20 Prague 4, Czech Republic.
FAU - Pavkova, Ivona
AU  - Pavkova I
AD  - From the double daggerDepartment of Molecular Pathology and Biology, Faculty of Military 
      Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic.
FAU - Rehulka, Pavel
AU  - Rehulka P
AD  - From the double daggerDepartment of Molecular Pathology and Biology, Faculty of Military 
      Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic.
FAU - Krocova, Zuzana
AU  - Krocova Z
AD  - From the double daggerDepartment of Molecular Pathology and Biology, Faculty of Military 
      Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic.
FAU - Hozak, Pavel
AU  - Hozak P
AD  -  section signInstitute of Molecular Genetics ASCR v.v.i., Microscopy Centre, Electron 
      Microscopy Core Facility, 142 20 Prague 4, Czech Republic.
AD  -  paragraph signInstitute of Molecular Genetics ASCR v.v.i., Department of Biology of the Cell 
      Nucleus, 142 20 Prague 4, Czech Republic.
FAU - Santic, Marina
AU  - Santic M
AD  -  ||Department of Microbiology, Faculty of Medicine, University of Rijeka, 51000 
      Rijeka, Croatia.
FAU - Stulik, Jiri
AU  - Stulik J
AD  - From the double daggerDepartment of Molecular Pathology and Biology, Faculty of Military 
      Health Sciences, University of Defence, 500 01 Hradec Kralove, Czech Republic; 
      jiri.stulik@unob.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171018
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Dendritic Cells/*metabolism/microbiology
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Female
MH  - *Francisella tularensis
MH  - Mice, Inbred C57BL
MH  - Phosphorylation
MH  - Tularemia/*metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
PMC - PMC5750852
EDAT- 2017/10/20 06:00
MHDA- 2019/01/04 06:00
PMCR- 2019/01/01
CRDT- 2017/10/20 06:00
PHST- 2017/06/27 00:00 [received]
PHST- 2017/09/22 00:00 [revised]
PHST- 2017/10/20 06:00 [pubmed]
PHST- 2019/01/04 06:00 [medline]
PHST- 2017/10/20 06:00 [entrez]
PHST- 2019/01/01 00:00 [pmc-release]
AID - S1535-9476(20)32285-4 [pii]
AID - RA117.000160 [pii]
AID - 10.1074/mcp.RA117.000160 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2018 Jan;17(1):81-94. doi: 10.1074/mcp.RA117.000160. Epub 
      2017 Oct 18.