PMID- 29046617
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1522-0613 (Print)
IS  - 1937-3791 (Electronic)
IS  - 1522-0613 (Linking)
VI  - 17
DP  - 2016
TI  - A fluorescence in situ hybridization (FISH) protocol for stickleback tissue.
PG  - 603-617
AB  - BACKGROUND: Threespine stickleback are an important model for behaviour and 
      evolutionary studies. A growing number of quantitative trait loci (QTL) and gene 
      expression studies are identifying genes related to ecologically important traits 
      in sticklebacks. In order to visualize the expression of candidate genes, we 
      developed a fluorescence in situ hybridization (FISH) protocol. METHODS: We 
      present a protocol for FISH on fresh or flash-frozen dissected tissue, using 
      either cryo- or paraffin embedding. The protocol covers probe design guidelines 
      and synthesis, sample embedding, sectioning, and the hybridization process. The 
      protocol is optimized for brain tissue. Key steps for modifying the protocol for 
      other tissues are noted. RESULTS: The FISH protocol resulted in specific 
      labelling under all combinations of dissection and embedding conditions. Paraffin 
      embedding preserved morphology better than cryo-embedding. We provide 
      representative results showing the expression of glial fibrillary acidic protein 
      (GFAP), oxytocin receptor (OXTR), and tyrosine hydroxylase (TH) in the brain.
FAU - James, Noelle
AU  - James N
AD  - Neuroscience Program, University of Illinois at Urbana-Champaign, Urbana, 
      Illinois, USA.
FAU - Liu, Xiaochen
AU  - Liu X
AD  - Carl R. Woese Institute for Genomic Biology, University of Illinois at 
      Urbana-Champaign, Urbana, Illinois, USA.
FAU - Bell, Alison
AU  - Bell A
AD  - Neuroscience Program, University of Illinois at Urbana-Champaign, Urbana, 
      Illinois, USA.
AD  - School of Integrative Biology, University of Illinois at Urbana-Champaign, 
      Urbana, Illinois, USA.
LA  - eng
GR  - R01 GM082937/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Evol Ecol Res
JT  - Evolutionary ecology research
JID - 100961530
PMC - PMC5642962
MID - NIHMS869359
OTO - NOTNLM
OT  - Gasterosteus aculeatus
OT  - RNA
OT  - fluorescence in situ hybridization
OT  - fluorescence methods
OT  - gene expression
OT  - gene localization
OT  - in situ hybridization
OT  - threespine stickleback
EDAT- 2016/01/01 00:00
MHDA- 2016/01/01 00:01
PMCR- 2017/10/16
CRDT- 2017/10/20 06:00
PHST- 2017/10/20 06:00 [entrez]
PHST- 2016/01/01 00:00 [pubmed]
PHST- 2016/01/01 00:01 [medline]
PHST- 2017/10/16 00:00 [pmc-release]
PST - ppublish
SO  - Evol Ecol Res. 2016;17:603-617.